Navigation Links
P(acman) takes a bite out of deciphering Drosophila DNA

P(acman) ?a new method of introducing DNA into the genome of fruit flies or Drosophila ?promises to transform the ability of scientists to study the structure and function of virtually all the fly’s genes, and the method may be applicable to other frequently studied organisms such as mice, said its Baylor College of Medicine developers in an article in the current issue of the journal Science.

“P(acman) overcomes a key limitation of currently available methods because it allows you to study large chunks of DNA in vivo,?said Dr. Hugo Bellen, professor of molecular and human genetics at Baylor College of Medicine and director of the program in developmental biology. He is also a Howard Hughes Medical Institute investigator. The new technique allows researchers to study large genes and even gene complexes in the fruit fly, which was not possible before.

P/phiC31 artificial chromosome for manipulation, or P(acman), combines three recently developed technologies: a specially designed bacterial artificial chromosome (BAC) that allows maintenance of large pieces of DNA in bacteria, recombineering that allows the manipulation of large pieces of DNA that can then be inserted into the genome of the fly at a specific site using phiC31-mediated transgenesis.

It is a new technique with far-reaching promise, said Bellen.

P(acman) overcomes certain obstacles that have hampered research. It allows the cloning of large pieces of DNA to be used to transform the genome, and it permits that DNA to be inserted into specific places in the genome. Bellen credits the report’s first author, Koen J.T. Venken, a graduate student in the BCM Program in Developmental Biology, with putting the technologies together to come up with a new methodology in the field.

Current technology has certain problems for researchers seeking to understand the structure and function of genes, said Bellen. Often, when scientists breed flies that lack a particular gene and then try to put that gene back into the fly, it inserts itself randomly into the genetic blueprint.

In some cases, it makes too much protein, and in others, too little. In other instances, it may disrupt the message from another gene.

“You are really comparing apples and oranges when you do this,?said Bellen. The technique is also limited to small DNA chunks.

“Koen set out to develop a new transgenesis system using the three techniques,?said Bellen.

The bacterial artificial chromosome, or BAC, he used allows the scientist to maintain large chunks of DNA in the bacteria, but it is present in only one or few copies. However, the bacteria can be induced to produce many copies of the DNA when needed.

Koen then integrated a technique called “recombineering?into the strategy, which facilitates the scientist to clone large chunks of DNA and subsequently allows them to make specific mutations anywhere he or she wants in the gene.

The third technique allows the researcher to pinpoint where he or she wants to the mutant gene to go in the genetic blueprint of the fly, eliminating the apples-and-oranges problem. This third technique ?phiC31 ?works also in mouse and human cells, implying that this new technique could be used in those cells as well.
'"/>

Source:Baylor College of Medicine


Related biology news :

1. NJIT Presidential Award winner takes stem cell research another step
2. Unique library of plant genes germinates, takes root at UNC
3. New influenza vaccine takes weeks to mass produce
4. Oops! Researchers publish new findings on the brains response to costly mistakes
5. Blood flow in brain takes a twist, affecting views of Alzheimers
6. HIV vaccine takes different tack to boosting immune response
7. Taking evolutions temperature: Researchers pinpoint the energy it takes to make a species
8. For Stanford scientists, RNAi gene therapy takes two steps forward, one step back
9. Pitt phage hunter takes on tuberculosis
10. The brain, traffic and nano-circuits -- e-Science takes on major challenges
11. Diabetes research takes wing thanks to long-lived fruit fly

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/2/2016)... The Department of Transport Management (DOTM) of ... project, for the , Supply and Delivery of ... Infrastructure , to Decatur , ... Management Solutions. Numerous renowned international vendors participated in the tendering ... selected for the most compliant and innovative solution. The contract ...
(Date:6/1/2016)... 2016 Favorable Government Initiatives Coupled ... Criminal Identification to Boost Global Biometrics System Market Through ... Research report, " Global Biometrics Market By Type, ... Opportunities, 2011 - 2021", the global biometrics market is ... account of growing security concerns across various end use ...
(Date:5/16/2016)... YORK , May 16, 2016   EyeLock ... solutions, today announced the opening of an IoT Center ... to strengthen and expand the development of embedded iris ... an unprecedented level of convenience and security with unmatched ... authenticate one,s identity aside from DNA. EyeLock,s platform uses ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Mass. , June 23, 2016   ... development of novel compounds designed to target cancer ... napabucasin, has been granted Orphan Drug Designation from ... the treatment of gastric cancer, including gastroesophageal junction ... stemness inhibitor designed to inhibit cancer stemness pathways ...
(Date:6/23/2016)... 2016  The Prostate Cancer Foundation (PCF) is pleased to announce ... cures for prostate cancer. Members of the Class of 2016 were selected from ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case ... Denmark detail how a patient who developed lymphedema after being treated for breast cancer ... could change the paradigm for dealing with this debilitating, frequent side effect of cancer ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, the only ... Center and will showcase its product’s latest features from June 26 to June ... scientific poster on Disrupting Clinical Trials in The Cloud during the conference. ...
Breaking Biology Technology: