Navigation Links
OHSU study says stem cell 'fusion' occurs in tumors

An Oregon Health & Science University study is adding credence to an increasingly popular theory that fusion is what bonds stem cells with bone marrow cells to regenerate organ tissue.

Scientists in the OHSU School of Medicine found that transplanted cells derived from adult bone marrow can fuse with intestinal stem cells of both normal and diseased tissue comprising the cellular lining of intestinal walls, known as the epithelium. The findings, reported recently in the Proceedings of the National Academy of Sciences, point to the integral role of bone marrow-derived cells in not only regeneration of damaged tissue, but also disease progression.

"It's the first observation that there's fusion at the level of stem cells," said the study's corresponding author, Melissa Wong, Ph.D., assistant professor of dermatology, and cell and developmental biology. "Second, we're seeing cell fusion in tumors and we believe that this concept is an underappreciated mechanism for promoting tumor growth. Our findings have implications on how tissues regenerate and how, in the process of this regeneration, cells may become prone to future problems. "

Although the tumor in her study did not "initiate" tumors or become malignant, Wong believes the fusion process is one explanation for how tumors acquire genetic instability and have the potential to give rise to malignant cancer.

One promising result could be in better understanding the careful balance between rapid and effective regeneration after tissue injury, and minimizing the risk of cancer. This balance can be examined in mouse models of inflammatory bowel disease, where, like humans, epithelial damage and chronic tissue repair occurs.

"Ten percent of patients with inflammatory bowel disease go on to get colorectal cancer," Wong said. "We think that the bone marrow cells aid in repair of the epithelium, but the cell fusion hybrids that remain are generally unstable. Fusion may be an underl ying molecular explanation of why these cells might be more susceptible to cancer development."

In the PNAS study, Wong and her colleagues transplanted bone marrow cells from female mice into a male mouse model of intestinal cancer. The donor-derived cells were readily detected in the intestinal tumors of the male mice.

To show that the transplanted donor cells fused with the tumor cells, the scientists detected proteins or markers from both the female donor and male recipient cells in tumors. The only way both donor and recipient markers could be present in a single cell, or colocalized, would be if the two cells fused.

One test to prove cell fusion involved looking for the presence of Y chromosomes, or male-characteristic DNA, within the female donor cells marked with a green fluorescent protein. About 60 percent of the epithelial cells were positive for both donor and recipient cell markers. A second test confirmed colocalization using a confocal microscope, which can scan a single cell layer of intestinal cells for the presence of both the green fluorescent protein, indicating female donor cells, and an enzyme from the male recipient cells

Wong said, "Our paper is unique because it's the first example that cell fusion occurs in the epithelial compartment of a tumor."

Other OHSU studies in recent years are giving weight to the fusion theory. A series of discoveries since 2000 by study co-author Markus Grompe, M.D., professor of molecular and medical genetics, and pediatrics, OHSU School of Medicine, and director of OHSU's Oregon Stem Cell Center, showed that blood-forming stem cells derived from bone marrow, called hematopoietic stem cells, cure liver disease in mice through cell fusion rather than transdifferentiation of the transplanted stem cells.

Wong acknowledges fusion is still a new concept. But it's gaining popularity as a way of explaining the molecular mechanisms that may be at work in regenerating rapidly turning over damaged tissue, or may even be implicated in disease progression.

"The intestinal lining is the protective barrier between your body and the outside environment. Therefore, it sees all of the offensive contaminants that you ingest, from the beer you drink to the charbroiled hamburger you eat, that can induce mutations in the cells. The gut protects itself by rapidly turning over its epithelial cells. Plus, when there is damage to the surface lining, it is critical that regeneration and reestablishment of the barrier occurs rapidly. We think that cell fusion with bone marrow cells is the body's way of jump-starting that regeneration of the epithelium," she said.


'"/>

Source:Oregon Health & Science University


Related biology news :

1. Bioartificial kidney under study at MCG
2. W.M. Keck Foundation funds study of friendly microbes
3. Yellowstone microbes fueled by hydrogen, according to U. of Colorado study
4. Genome-wide mouse study yields link to human leukemia
5. Clam embryo study shows pollutant mixture adversely affects nerve cell development
6. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
7. Same mutation aided evolution in many fish species, Stanford study finds
8. Sequencing of marine bacterium will help study of cell communication
9. Genetically modified rice in China benefits farmers health, study finds
10. A new study examines how shared pathogens affect host populations
11. NYU study reveals how brains immune system fights viral encephalitis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/2/2016)... 2016   SoftServe , a global digital ... an electrocardiogram (ECG) biosensor analysis system for continuous ... asset. The smart system ensures device-to-device communication between ... and mobile devices to easily ,recognize, and monitor ... vehicle technology advances, so too must the security ...
(Date:11/29/2016)... 29, 2016   Neurotechnology , a ... recognition technologies, today released FingerCell 3.0, a ... solutions that run on low-power, low-memory microcontrollers. ... less than 128KB of memory, enabling it ... have limited on-board resources, such as: mobile ...
(Date:11/22/2016)... India , November 22, 2016 According to ... (Fingerprint, IRIS, Palm Print, Face, Vein, Signature, Voice), Multi-Factor), Component (Hardware and ... 2022", published by MarketsandMarkets, the market is expected to grow from USD ... a CAGR of 16.79% between 2016 and 2022. ... ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... , ... December 08, 2016 ... ... the commercial launch of flexible packaging for their exceptionally efficient human mesenchymal ... system extends RoosterBio’s portfolio of bioprocess media products engineered to radically streamline ...
(Date:12/8/2016)... 2016  Soligenix, Inc. (OTCQB: SNGX) (Soligenix or ... developing and commercializing products to treat rare diseases ... today the long-term follow-up data from its Phase ... Innate Defense Regulator (IDR), in the treatment of ... patients undergoing chemoradiation therapy (CRT).  The additional 12-month ...
(Date:12/8/2016)... Savannah River Remediation LLC group evaluated ... NT-MAX Lake & Pond Sludge and Muck ... conjunction with Hexa Armor/ Rhombo cover manufactured by ... Discharge Elimination System requirements. The Savannah ... of elevated pH levels, above 8.5, especially during ...
(Date:12/8/2016)... 2016   Biocept, Inc . (NASDAQ: ... clinically actionable liquid biopsy tests to improve the ... featuring its Target Selector™ Circulating Tumor Cell platform ... detection of actionable biomarkers in patients with metastatic ... Sara Cannon Research Institute (SCRI), the research arm ...
Breaking Biology Technology: