Navigation Links
Novel method reveals how menthol discovery could point towards new or improved pain therapies

This new understanding could lead to potential advances in pain therapy, the researchers said. Moreover, the scientists envision that their method may be potentially useful in studying the activation mechanism of other drugs and proteins.

"Because our ability to sense temperature is closely linked to our ability to sense pain, it is not surprising that the misregulation of temperature-activated ion channels can result in chronic pain syndromes," said Ardem Patapoutian, associate professor at Scripps Research and member of GNF, who directed the research. "In fact, some of these ion channels are considered targets to treat chronic inflammatory and neuropathic pain indications. Understanding how small molecules such as menthol affect the function of these proteins could be crucial in designing future drugs that can either activate or block them."

The study was released in an advanced online version by the journal Nature Neuroscience. It will be published in the journal's April edition (Vol. 9, No. 4).

Utilizing a novel mutagenesis and high-throughput screening approach, the study assayed 14,000 TRPM8 mutants to find mutants that were not enhanced by menthol but were otherwise functioning normally. The scientists' analysis pinpointed a potential interaction site for menthol, as well as a site that translates binding information to ion channel activity.

Ion channels are proteins found in the cell membrane that can form a tunnel or channel that allows specific ions to move across the membrane. When activated, the channel opens, allowing an influx of calcium ions into the axon, an electrical signal that alerts the neuron, which relays the message to the brain.

Research Associate Michael Bandell, the lead author of the study, noted, "It's a well established method to mutate individual amino acid residues in an ion channel protein and examine the effect that these mutations have on the channel's function. However, the laborious n ature of these experiments limits the number of mutant ion channels that can be made and analyzed. Our new high-throughput screening methodology allowed us to analyze 14,000 mutants out of which we isolated five that specifically affected menthol activity. Our experiments yielded significant insights into the functional elements of TRPM8 ion channel protein that would have been difficult to obtain using other mutagenesis methods."

Because the methodology can be used to screen for activation or inhibition, Bandell added, it could prove to be useful as a general method to analyze the mechanism by which drugs can activate or inhibit ion channels or other receptors. Specifically, the new methodology could be used to identify amino acid residues in certain ion channel proteins and G-protein coupled receptors (proteins involved in stimulus-response pathways) that are involved in the interaction with small molecules that affect their function.


Source:Scripps Research Institute

Related biology news :

1. Novel Asthma Study Shows Multiple Genetic Input Required; Single-gene Solution Shot Down
2. Novel technology detects human DNA mutations
3. Novel antiviral technology inhibits RSV infection in mice
4. Novel Enzyme Shows Potential As An Anti-HIV Target
5. Novel Therapy Tested in Mice Could Chase Away Cat Allergies
6. Discovery Could Lead To Novel Approaches In HIV Treatment
7. Novel ultrafast laser detection of cancer cells also may improve understanding of stem cells
8. Research Using Mouse Models Reveals A Novel Key Player In The Initiation Of Colon Cancer
9. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma
10. Novel live reporting system to track cells
11. Field of beams - Novel system uses polarized light pulses to reveal crop health
Post Your Comments:

(Date:10/12/2015)... Research and Markets ( ) ... Recognition Market by Component (Hardware & Software), Product ... Industry (Travel & Immigration, Military & Defense, & ... report to their offering. ... Market worth 3627.90 Million USD by 2020 ...
(Date:10/7/2015)... 8, 2015 --> ... Cards (FPC) during third quarter 2015 amounted to around 960 ... 860 MSEK that was communicated 20 August 2015. ... delivery capacity and a continued growing demand for the company,s ... to be higher than during the third quarter. The revenue ...
(Date:10/2/2015)... ) has ... Law Using Biometrics" report to their offering. ... addition of the "Enforcing the Law Using ... Research and Markets ( ) has ... Law Using Biometrics" report to their offering. ...
Breaking Biology News(10 mins):
(Date:10/12/2015)... de octubre de 2015 El 8 de ... un récord en el congreso con su declaración acerca ... International Plasma Awareness Week (IPAW), que se celebrará del ... por la Plasma Protein Therapeutics Association (PPTA) ... , Aumentar la concienciación mundial acerca de la ...
(Date:10/12/2015)... , October 12, 2015 LabStyle ... Diabetes Management Solution, today announced its Medical Director, Dr. ... study at MobiHealth,s 5th EAI International Conference on ... healthcare through innovations in mobile and wireless technologies," the ... from October 14 - 16, 2015. The ...
(Date:10/12/2015)... ... October 12, 2015 , ... Dr. Carl Peck, MD, ... the firm as a Premier Expert consultant. NDA Partners Premier Experts are ... value to the company's clients. Premier Experts collaborate to design and implement ...
(Date:10/11/2015)... ... October 11, 2015 , ... Intelligent Implant Systems, ... launched and multiple surgeries have been completed with this new posterior thoracolumbar spinal ... & Spine Center of the Carolinas. The Revolution™ Spinal System pioneers a ...
Breaking Biology Technology: