Navigation Links
Novel method reveals how menthol discovery could point towards new or improved pain therapies

This new understanding could lead to potential advances in pain therapy, the researchers said. Moreover, the scientists envision that their method may be potentially useful in studying the activation mechanism of other drugs and proteins.

"Because our ability to sense temperature is closely linked to our ability to sense pain, it is not surprising that the misregulation of temperature-activated ion channels can result in chronic pain syndromes," said Ardem Patapoutian, associate professor at Scripps Research and member of GNF, who directed the research. "In fact, some of these ion channels are considered targets to treat chronic inflammatory and neuropathic pain indications. Understanding how small molecules such as menthol affect the function of these proteins could be crucial in designing future drugs that can either activate or block them."

The study was released in an advanced online version by the journal Nature Neuroscience. It will be published in the journal's April edition (Vol. 9, No. 4).

Utilizing a novel mutagenesis and high-throughput screening approach, the study assayed 14,000 TRPM8 mutants to find mutants that were not enhanced by menthol but were otherwise functioning normally. The scientists' analysis pinpointed a potential interaction site for menthol, as well as a site that translates binding information to ion channel activity.

Ion channels are proteins found in the cell membrane that can form a tunnel or channel that allows specific ions to move across the membrane. When activated, the channel opens, allowing an influx of calcium ions into the axon, an electrical signal that alerts the neuron, which relays the message to the brain.

Research Associate Michael Bandell, the lead author of the study, noted, "It's a well established method to mutate individual amino acid residues in an ion channel protein and examine the effect that these mutations have on the channel's function. However, the laborious n ature of these experiments limits the number of mutant ion channels that can be made and analyzed. Our new high-throughput screening methodology allowed us to analyze 14,000 mutants out of which we isolated five that specifically affected menthol activity. Our experiments yielded significant insights into the functional elements of TRPM8 ion channel protein that would have been difficult to obtain using other mutagenesis methods."

Because the methodology can be used to screen for activation or inhibition, Bandell added, it could prove to be useful as a general method to analyze the mechanism by which drugs can activate or inhibit ion channels or other receptors. Specifically, the new methodology could be used to identify amino acid residues in certain ion channel proteins and G-protein coupled receptors (proteins involved in stimulus-response pathways) that are involved in the interaction with small molecules that affect their function.


'"/>

Source:Scripps Research Institute


Related biology news :

1. Novel Asthma Study Shows Multiple Genetic Input Required; Single-gene Solution Shot Down
2. Novel technology detects human DNA mutations
3. Novel antiviral technology inhibits RSV infection in mice
4. Novel Enzyme Shows Potential As An Anti-HIV Target
5. Novel Therapy Tested in Mice Could Chase Away Cat Allergies
6. Discovery Could Lead To Novel Approaches In HIV Treatment
7. Novel ultrafast laser detection of cancer cells also may improve understanding of stem cells
8. Research Using Mouse Models Reveals A Novel Key Player In The Initiation Of Colon Cancer
9. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma
10. Novel live reporting system to track cells
11. Field of beams - Novel system uses polarized light pulses to reveal crop health
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... BEACH GARDENS, Fla. , April 11, 2017 ... identity management and secure authentication solutions, today announced ... contract by Intelligence Advanced Research Projects Activity (IARPA) ... for IARPA,s Thor program. "Innovation has ... onset and IARPA,s Thor program will allow us ...
(Date:4/11/2017)... NXT-ID, Inc. (NASDAQ:   NXTD ) ... appointment of independent Directors Mr. Robin D. Richards ... Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive ... their guidance and benefiting from their considerable expertise as we ...
(Date:4/5/2017)... -- Today HYPR Corp. , leading innovator in ... the HYPR platform is officially FIDO® Certified . ... that empowers biometric authentication across Fortune 500 enterprises and ... 15 million users across the financial services industry, however ... suites and physical access represent a growing portion of ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... 2017 , ... At its national board meeting in North ... the co-founder, CEO and chief research scientist of Minnesota-based Advanced Space Technology and ... ARCS Alumni Hall of Fame . ASTER Labs is a technology development ...
(Date:10/11/2017)... Oct. 11, 2017  VMS BioMarketing, a leading provider of ... oncology Clinical Nurse Educator (CNE) network, which will launch this ... communication among health care professionals to enhance the patient care ... staff, and other health care professionals to help women who ... ...
(Date:10/10/2017)... ... , ... San Diego-based team building and cooking events company, Lajollacooks4u, has unveiled ... bold new look is part of a transformation to increase awareness, appeal to new ... , It will also expand its service offering from its signature gourmet cooking classes ...
(Date:10/10/2017)... 2017 SomaGenics announced the receipt of a ... RealSeq®-SC (Single Cell), expected to be the first commercially ... microRNAs) from single cells using NGS methods. The NIH,s ... accelerate development of approaches to analyze the heterogeneity of ... techniques for measuring levels of mRNAs in individual cells ...
Breaking Biology Technology: