Navigation Links
Nobel Laureate finds 'elegant' explanation for DNA transcribing enzyme's high fidelity

nucleotide,?Kornberg said.

The team traced that protein density back to a portion of the polymerase II enzyme: the trigger loop.

“Of the 14 crystal structures now reported in which the trigger loop was observed, only in two is it seen in that location, directly beneath the NTP,?Kornberg said. Those were the only two crystals in which the NTP was correctly matched to the DNA template, evidence of the trigger loop’s “clear relationship to NTP selection.?

Further study revealed that, when a matching NTP reaches the addition site, the trigger loop swings from its usual position some distance away until it rests parallel to the NTP. It then forms a network of interactions—some 20 to 30 in all—with components of the NTP, a process that serves to “recognize all features of the NTP in the addition site and detect its precise location,?the researchers reported.

“The specificity is a result of the alignment with the NTP that is critically dependent upon the base, sugar, phosphate and location when the trigger loop swings into position,?Kornberg said. “If it is misaligned even slightly, that set of contacts cannot occur.?

As a consequence of that alignment, to angstrom (a unit of length equal to one hundred millionth of a centimeter) precision, a histidine side chain of the trigger loop rests on the ß phosphate, the chemical constituent that must have its bond broken in order for the NTP to join the RNA chain through the formation of a phosphodiester bond, Kornberg said. The finding suggested the side chain acts as a trigger for bond formation.

The whole decision-making process occurs extremely rapidly, he added, on the order of picoseconds. A picosecond is one trillionth of a second.

“The basis for the extraordinary specificity with which RNA polymerases transcribe DNA lies in a structural element termed the trigger loop, which makes both direct and indirect contact with all features of the nucleotide in the polymer
'"/>

Source:Cell Press


Page: 1 2 3 4

Related biology news :

1. Papers of DNA Pioneer and Nobel Laureate Francis Crick Added to National Library of Medicine’s Profiles in Science Web Site
2. Nobelist discovers antidepressant protein in mouse brain
3. Comments, experts and background on the 2006 Nobel Prize in chemistry
4. New research says winning a Nobel Prize adds nearly 2 years to your lifespan
5. Current human embryonic stem cell lines contaminated UCSD/Salk team finds
6. Study finds more than one-third of human genome regulated by RNA
7. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
8. Same mutation aided evolution in many fish species, Stanford study finds
9. NC State scientist finds soft tissue in T. rex bones
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. Genetically modified rice in China benefits farmers health, study finds
Post Your Comments:
(Date:10/15/2014)... risk from strains of influenza virus increases with the ... become complacent that the most substantial threats have been ... pandemics arise when a new virus strain – against ... spreads in the human population. There have been five ... of which – the 1918 Spanish Flu – cost ...
(Date:10/15/2014)... 15, 2014 NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market releases photos and video of ... on Monday October 13 th . Gino ... and angel investor Mr. Chad A. Verdi rang ... NXT-ID thanked his investors and employees "for their work and dedication ...
(Date:10/14/2014)... 2014)—It,s been millions of years since T. rex ... by Ohio University scientists is breathing life back into ... dinosaur snouts. The research has important implications for how ... to enhance the sense of smell and cool their ... Ohio University doctoral student Jason Bourke, lead author of ...
Breaking Biology News(10 mins):Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 3Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3
... UK scientist has discovered clumps of previously-unreported callus hairs ... and closely-related varieties, which may have storage implications for ... apples is really exciting, says Dr Mary Parker of ... for about as long as human history and Fuji ...
... DALLAS March 25, 2008 Researchers at ... $6.5 million grant from the National Institute of ... anti-microbial compound to target bacterial pathogens such as ... drugs are available in the marketplace, new ones ...
... humans, are true farmers. The difference is that ants ... Sen Brady at the Smithsonians National Museum of Natural ... issue of the journal Proceedings of the National Academy ... of ants and how these abilities have evolved throughout ...
Cached Biology News:Popular apple variety harbors unusual cell growth 2NIH awards $6.5 million grant to UT Southwestern to develop new antibiotic 2Smithsonian's National Museum of Natural History reveals ants as fungus farmers 2
(Date:10/22/2014)... 22, 2014 Research and Markets ... "Global DNA Diagnostics Market (Product types, Application, Technology, ... Trends, Company Profiles, Demand, Insights, Analysis, Research, Report, ... report to their offering. This ... techniques, end users and Geography) Global Size, Industry ...
(Date:10/22/2014)... The North American crystal oscillator market report defines ... and forecast of revenue. This market was valued at ... $623.6 million by 2018, at a CAGR of 3.3% ... of the North American crystal oscillator market report, to ... also provides a glimpse of the segmentation of the ...
(Date:10/22/2014)... 22, 2014 WriteResult, LLC – ... has been working with a team of researchers from ... Farms Community Farming Collaborative to provide electronic questionnaire data ... The study, which started in May, aims to evaluate ... participants weekly exposure to and participation in urban agriculture ...
(Date:10/22/2014)... (PRWEB) October 22, 2014 Physicians ... its cardiovascular pharmacogenetics menu, which enables healthcare providers ... With PCLS’s evidence-based results, healthcare providers are ... optimize their therapy, while minimizing risks for adverse ... U.S., according to the FDA [1] more than ...
Breaking Biology Technology:Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 2Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 3The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 2The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 3The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 4myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3
... Implementation of a controversial web-based software system to manage ... proceed, a report on an in-depth assessment of the ... suggests that the UW System continue to work with ... the Appointments, Payroll and Benefits System (ABPS) project, which ...
... - Trying to find a vaccine for AIDS is tricky ... places on a sprawling university campus, it's downright daunting. , ... Laboratory opened on Sept. 16, the prospects for eventual success ... on a technique that may hold greater promise to prevent ...
... Wisconsin companies that won more than a combined $42 ... Business Technology Transfer (STTR) awards over the last two ... Madison. , ,The awards banquet will be preceded by ... focus on SBIR/STTR funding opportunities. The keynote speaker at ...
Cached Biology Technology:Report gives nod to controversial Lawson project 2Report gives nod to controversial Lawson project 3Report gives nod to controversial Lawson project 4New digs for UW AIDS researchers 2