Navigation Links
Nobel Laureate finds 'elegant' explanation for DNA transcribing enzyme's high fidelity

res include an unwound region of about 15 base pairs of the DNA with some eight residues of the RNA transcript hybridized with the DNA in the center of the “transcription bubble.?The enzyme polymerases involved are capable of moving both forward and backward on the DNA. Forward movement is favored by the binding of NTPs, while backtracking occurs especially when the enzyme encounters an impediment, such as damaged DNA.

Kornberg’s group captured the first picture of the polymerase II transcribing complex by X-ray crystallography in 2001. Those structures revealed the complex with a nucleotide still in the enzyme’s addition site, just after it had been added to the RNA transcript.

Later X-ray structures revealed the transcribing complex with the addition site available for entry of a matched NTP. Those crystals uncovered a second NTP-binding site on the transcribing enzyme, dubbed the entry site. While all NTPs can bind the entry site, only an NTP matched for base-pairing with the DNA template binds the addition site for attachment to the growing RNA strand, Kornberg said.

Yet the question of how the enzyme achieves such a high degree of discrimination between matched and mismatched NTPs remained unanswered.

The chemical attraction alone between RNA bases—adenine, cytosine, guanine, and uracil—and their complementary bases on the DNA template strand is far from sufficient to account for the incredible selectivity of polymerase II, Kornberg said. And the scientists didn’t know either how the polymerase avoids substituting the NTPs that constitute DNA for the correct RNA building blocks, molecules that differ by only one oxygen atom.

In search of an explanation in the current study, the researchers screened hundreds of crystals to achieve higher data quality and resolution than ever before.

“In the course of the work, we saw something that had never been noticed before?additional protein density beneath the matching
'"/>

Source:Cell Press


Page: 1 2 3 4

Related biology news :

1. Papers of DNA Pioneer and Nobel Laureate Francis Crick Added to National Library of Medicine’s Profiles in Science Web Site
2. Nobelist discovers antidepressant protein in mouse brain
3. Comments, experts and background on the 2006 Nobel Prize in chemistry
4. New research says winning a Nobel Prize adds nearly 2 years to your lifespan
5. Current human embryonic stem cell lines contaminated UCSD/Salk team finds
6. Study finds more than one-third of human genome regulated by RNA
7. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
8. Same mutation aided evolution in many fish species, Stanford study finds
9. NC State scientist finds soft tissue in T. rex bones
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. Genetically modified rice in China benefits farmers health, study finds
Post Your Comments:
(Date:9/18/2014)... stressed they are often grouchy, grumpy, nasty, distracted or ... EPFL have just highlighted a fundamental synaptic mechanism that ... of social skills and cognitive impairment. When triggered by ... the brain. This was revealed by a work published ... team went to look for answers in a region ...
(Date:9/18/2014)... in the protective tentacles of host anemones, but new ... of kilometres across the open ocean. Although the process ... this is the first time that the high level ... , Dr Steve Simpson, Senior Lecturer in Marine Biology ... colleagues from the Australian Research Council Centre of Excellence ...
(Date:9/17/2014)... doctoral student have designed a device based on a ... and dew., The device could provide water in drought-stricken ... Cheng Luo, professor in the Mechanical & Aerospace Engineering ... College of Engineering department, published "Bioinspired Plate-Based Fog Collectors" ... Society) Applied Materials & Interfaces ...
Breaking Biology News(10 mins):How stress tears us apart 2Expedition finds Nemo can travel great distances to connect populations 2Expedition finds Nemo can travel great distances to connect populations 3Shorebird's beak inspires UT Arlington research on water collection 2
... mothers than duller birds, according to a new study led ... see ultra-violet (UV) light. While the crown of a blue ... has the added dimension of appearing UV-reflectant. , The three-year ... University of California Davis, USA and the University of Glasgow, ...
... Dartmouth researchers has uncovered a protein that plays a vital ... key step in improving the production and quality of crops ... journal PNAS . The team included researchers ... Lausanne. Plant roots use their endodermis, or inner skin, ...
... stem cells and DNA-cutting protein from meningitis bacteria, researchers ... have created an efficient way to target and repair ... Aug. 12, 2013) in the Proceedings of the ... the novel technique is much simpler than previous methods ...
Cached Biology News:Bright birds make good mothers 2New gene repair technique promises advances in regenerative medicine 2
(Date:9/18/2014)... Research and Markets has announced the addition ... Report, 2014-2017" report to their offering. ... differentiate into specialized cells and can divide (through mitosis) ... be applied to treatment of cardiovascular diseases, leukemia (a ... or lesion of liver, kidney and other parenchymal organs, ...
(Date:9/18/2014)... TX (PRWEB) September 18, 2014 ... a FM-4910 listed material, to stay competitive in ... These high-performance materials ultimately impact product quality ... President and owner. , Established in 1980 in ... strong as many fabricators have left the business. ...
(Date:9/18/2014)... SEATTLE , Sept. 18, 2014 /PRNewswire/ - Oncothyreon ... pricing of previously announced concurrent but separate underwritten offerings ... price to the public of $2.00 per share, for ... of its Series A Convertible Preferred Stock at a ... expected gross proceeds of $20 million. Each share of ...
(Date:9/18/2014)... SeqLL Inc., the owner of the ... the closing of a $1M Series-A funding round. ... Technologies, will increase the power of tSMS™ technology ... March 2013 by Daniel Jones, a former Field ... SeqLL has continued to offer Helicos’ sequencing technology, ...
Breaking Biology Technology:Global and Chinese Stem Cell Industry Report, 2014-2017 2Global and Chinese Stem Cell Industry Report, 2014-2017 3Leatherwood Plastics Selects Vycom’s Flametec PVC-C Material to Stay Competitive in Semiconductor and Lab Equipment Industries 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 3SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2
... MONTREAL , Feb. 1 /PRNewswire-FirstCall/ - ICBS Ltd. (OTCPK: ... TOGS(TM) 3000 bio-photonic incubation & detection systems, which the company will ... 100% accuracy in the detection of Total Coli forms and E. ... to introduce the new TOGS(TM) 3000 in the U.S, and overseas." ...
... The ... as a regulatory consultant after retiring from Eli Lilly & Co. Dr. ... human pharmaceutical products, including director of regulatory affairs and clinical research. Dr. ... submissions. , ...
... Data Demonstrating a 35% Overall Response Rate with a ... Relapsed or Relapsed/Refractory Waldenstrom,s Macroglobulinemia to be Published in ... Journal of Clinical Cancer Research , QUÉBEC CITY, ... AEZS ; TSX: AEZ) (the "Company"), a late-stage ...
Cached Biology Technology:BioSpec Global Solutions Inc, takes delivery of new TOG's 3000 Home edition for the U.S. and international markets 2Michael Langley, DVM, MBA, RAC Joins the Anson Group as a Regulatory Consultant 2Æterna Zentaris Partner Keryx Announces Positive Phase 2 Results for Perifosine as a Single Agent for the Treatment of Advanced Waldenstrom's Macroglobulinemia 2Æterna Zentaris Partner Keryx Announces Positive Phase 2 Results for Perifosine as a Single Agent for the Treatment of Advanced Waldenstrom's Macroglobulinemia 3Æterna Zentaris Partner Keryx Announces Positive Phase 2 Results for Perifosine as a Single Agent for the Treatment of Advanced Waldenstrom's Macroglobulinemia 4Æterna Zentaris Partner Keryx Announces Positive Phase 2 Results for Perifosine as a Single Agent for the Treatment of Advanced Waldenstrom's Macroglobulinemia 5
Imject Freund's Incomplete Adjuvant (FIA)...
... to slightly cloudy liquid. Yellow, oily, ... set, each containing 10 ml of ... following proportions by weight: 85% Drakeol ... emulsifier), and 0.1% Mycobacterium butyricum ...
... Although there have been many modifications to ... defined media, salt solutions still play an important ... to maintain the pH and osmotic balance in ... water and essential inorganic ions, is as valuable ...
Request Info...
Biology Products: