Navigation Links
New tool reveals secrets of migrating cells

the dye glow and the cells light up. A certain energy or color of laser light is needed to make the dye, in this case green fluorescent protein, glow. But high-frequency, short-wavelength visible light, like green, doesn't penetrate tissue as deeply as longer, redder wavelengths.

The idea behind two-photon imaging is that if you hit a dye molecule in a short period of time with two photons of light, each photon half the energy needed to excite it, the dye can absorb them together and then fluoresce. The less energetic, long-wavelength photons will go deeper into the tissue, cause less damage and scatter less, Robey said, essentially illuminating slices through the tissue that can be sharply imaged and stacked to produce a 3-D image of the cells in real time. The system they use employs an infrared laser emitting short intense pulses of 920 nanometer-wavelength light.

In the thymus, it's possible to view cells 400 microns inside the cortex, which is about 4/10 of a millimeter or more than a hundredth of an inch deep. In the current study, Witt limited her viewing to about 200 microns, though she says in some tissues less dense than the thymus, light could penetrate nearly a millimeter - deep enough to probe cell activity in most tissues.

Witt pointed out that obtaining a movie of cell movement is just the beginning. The human eye and brain can't pick out patterns of movement easily, so statistical techniques are needed to identify cells with different patterns of movement.

As an immunologist, Robey focuses on the lives of T cells produced in the thymus and distributed via the bloodstream to the lymph nodes, whence they move into the body's tissues. Her first use of two-photon imaging three years ago surprised her and many immunologists because it showed that thymocytes or immature T cells were highly mobile, traveling thousands of microns in an hour as they explore the thymus.

The new experiments, conducted primarily by Witt,
'"/>

Source:University of California - Berkeley


Page: 1 2 3 4 5

Related biology news :

1. A bacterial genome reveals new targets to combat infectious disease
2. First real-time view of developing neurons reveals surprises, say Stanford researchers
3. Examination of internal wiring of yeast, worm, and fly reveals conserved circuits
4. NYU study reveals how brains immune system fights viral encephalitis
5. Study reveals new technique for fingerprinting environmental samples
6. Study reveals dramatic difference between breast cancers in US and Africa
7. UCSD research reveals mechanism involved with type of fatal epilepsy
8. Study reveals candidate targets for anti-retroviral therapeutics
9. Chimp genome reveals a retroviral invasion
10. Survey reveals women and doctors arent talking about HPV
11. Bioinformatics reveals new gene regulation system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New tool reveals secrets migrating cells

(Date:5/18/2015)... -- Fingerprint Cards (FPC) has received an order ... FPC1035 from one if its module partners. Deliveries are planned ... and the sensors will be used by smartphone manufacturers in ... hereby amount to 740 MSEK to date. In addition to ... a number of smaller orders not separately communicated amounting to ...
(Date:5/11/2015)... , May 11, 2015  Through a well-rounded UAS delegation ... had a strong showing at AUVSI,s Unmanned 2015 conference ... of Ohio,s UAS industry met with ... from all points along the UAS ecosystem. ... (DDC) Vice President for Aerospace Rich Knoll . "If ...
(Date:5/7/2015)... GOTHENBURG, Sweden , May 7, 2015 /PRNewswire/ ... new touch fingerprint sensors, FPC1022 and FPC1035, FPC,s ... FPC1022 and FPC1035 are mainly considered for integration ... decreased size gives smartphone OEMs increased possibilities to ... portfolio. The decreased size also improves possibilities for ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2FPC Introduces its Smallest Touch Fingerprint Sensors to Date 2
... , MOUNTAIN VIEW, CA January 11, 2011 23andMe ... to be the 10 most interesting and significant genetic findings ... the role of genetics in personal health and human development. ... at a phenomenal rate," stated Anne Wojcicki, co-founder and CEO ...
... bit of a Jekyll and Hyde relationship with mercury, ... National Laboratory have made a discovery that ultimately could ... known that microbes in aquatic environments make methylmercury, a ... they also know that nature and other types of ...
... it cracks or otherwise fails, it proves brittle, shattering ... be toughthey resist shatteringbut are also relatively weak; they ... says Marios Demetriou, a senior research fellow at the ... being both strong and tougha combination called damage tolerance, ...
Cached Biology News:23andMe presents top 10 most interesting genetic findings of 2010 223andMe presents top 10 most interesting genetic findings of 2010 323andMe presents top 10 most interesting genetic findings of 2010 423andMe presents top 10 most interesting genetic findings of 2010 523andMe presents top 10 most interesting genetic findings of 2010 623andMe presents top 10 most interesting genetic findings of 2010 723andMe presents top 10 most interesting genetic findings of 2010 823andMe presents top 10 most interesting genetic findings of 2010 923andMe presents top 10 most interesting genetic findings of 2010 1023andMe presents top 10 most interesting genetic findings of 2010 1123andMe presents top 10 most interesting genetic findings of 2010 1223andMe presents top 10 most interesting genetic findings of 2010 1323andMe presents top 10 most interesting genetic findings of 2010 1423andMe presents top 10 most interesting genetic findings of 2010 1523andMe presents top 10 most interesting genetic findings of 2010 1623andMe presents top 10 most interesting genetic findings of 2010 1723andMe presents top 10 most interesting genetic findings of 2010 18Natural dissolved organic matter plays dual role in cycling of mercury 2Caltech-led team creates damage-tolerant metallic glass 2Caltech-led team creates damage-tolerant metallic glass 3Caltech-led team creates damage-tolerant metallic glass 4
(Date:5/21/2015)...  The EveryLife Foundation for Rare Diseases applauded ... Amy Klobuchar (D-MN) today for introducing the ... or OPEN ACT. Supported by more ... bipartisan legislation promises to rapidly bring hundreds of ... patients by incentivizing drug makers to "repurpose" approved ...
(Date:5/21/2015)... 21, 2015 W. R. Grace ... facility in Worms, Germany has received good manufacturing ... of the International Pharmaceutical Excipient Council (IPEC) Foundation. ... produce its SYLOID® FP brand of pharmaceutical grade ... following the Curtis Bay, Maryland (USA) and Sorocaba, ...
(Date:5/21/2015)... ANGELES , May 21, 2015  CytRx ... and development company specializing in oncology, today announced ... clinical trial with aldoxorubicin for the treatment of ... brain cancer.  The open-label, multisite trial is designed ... aldoxorubicin in patients whose tumors have progressed following ...
(Date:5/21/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/84975r/2015_global ) ... Global Survey on Flow Cytometry Adoption Trends" ... goal of this research is to analyze the ... information the survey seeks to collect include factors ... used applications for flow cytometers, respondents, most preferred ...
Breaking Biology Technology:Advocates Cheer Senate Leaders for Introducing Bipartisan Bill to Increase Number of Rare Disease Treatments 2Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 3CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 4CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 5CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 6CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 7Global Survey on Flow Cytometry Adoption Trends 2015 2
... (PBS-Bio) decodes the workings of drugs made by two private firms ... the American Association for Cancer Research. The conference runs April ... The presentations will be given on April 5. In ... a Belgian firm, affects two genes MCL1 and MYC. ...
... Ill. With the first observation of thermoelectric effects ... graphene transistors have a nanoscale cooling effect that reduces ... professor William King and electrical and computer engineering professor ... the April 3 advance online edition of the journal ...
... cannot be focused to spot sizes smaller than half ... approach to beat this diffraction limit is based on ... radiofrequency counterparts. They have the ability to concentrate (focus) ... orders of magnitude smaller than what conventional lenses can ...
Cached Biology Technology:TGen and PBS-Bio presents 2 abstracts at AACR conference 2Self-cooling observed in graphene electronics 2Transmission lines for nanofocusing of infrared light 2Transmission lines for nanofocusing of infrared light 3