Navigation Links
New tool reveals secrets of migrating cells

migrations have been inferred from observations of chemically fixed tissue at different stages of development.

"Two-photon imaging is going to change literally forever the way that we do biological science," said Witt, a developmental immunologist. "In the past, we'd take organs out, smush them up and basically do biochemistry in test tubes, or watch their behavior in a single layer of cells. It's an imaging revolution to be able to go into the native environment while keeping the intact organ alive and make movies of migrating cells."

With two-photon imaging, Witt and Robey identified thymus cells they dubbed beeliners moving nearly two centimeters - almost an inch - per hour, which is fast in the realm of cell movement. They think that these are cells that have received a signal committing them to be either a helper T cell - which aids other immune cells in fighting infections - or a killer T cell that seeks and destroys cells infected with virus.

On the other hand, uncommitted or immature T cells, what they call meanderers, wander slowly and apparently randomly around the outer layer, or cortex, of the thymus, perhaps in search of that life-altering signal.

Robey hopes to use two-photon imaging to investigate the signals responsible for changing these meanderers into purposeful beeliners that immediately leave the cortex for the interior medulla of the thymus.

"We're now at the point with this technology that we can begin to look at the movement of signaling molecules within the cells," she said.

Robey, with another colleague, Philippe Bousso, last year published a review in the journal Immunity describing the contributions two-photo imaging has made to the field. Robey and Witt publish their current study in the May 3 issue of the Public Library Of Science-Biology.

Two-photon imaging is a variation on the standard technique of labeling cells with fluorescent dye and then hitting them with a laser that makes
'"/>

Source:University of California - Berkeley


Page: 1 2 3 4 5

Related biology news :

1. A bacterial genome reveals new targets to combat infectious disease
2. First real-time view of developing neurons reveals surprises, say Stanford researchers
3. Examination of internal wiring of yeast, worm, and fly reveals conserved circuits
4. NYU study reveals how brains immune system fights viral encephalitis
5. Study reveals new technique for fingerprinting environmental samples
6. Study reveals dramatic difference between breast cancers in US and Africa
7. UCSD research reveals mechanism involved with type of fatal epilepsy
8. Study reveals candidate targets for anti-retroviral therapeutics
9. Chimp genome reveals a retroviral invasion
10. Survey reveals women and doctors arent talking about HPV
11. Bioinformatics reveals new gene regulation system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New tool reveals secrets migrating cells

(Date:7/31/2014)... adipocytes, or fat cells, are inherently different. Each of ... its own role in metabolism. In the human body, ... Its primary function is energy storage. On the other ... but are only found in a few places in ... special type of brown adipocytes, appear mixed with brown ...
(Date:7/30/2014)... the way for a breakthrough that may prevent ... to poisonous chemicalsparticularly those in pesticides and chemical ... issue of the journal ChemBioChem outlines ... commonly used in pesticides and warfare agents. The ... Engineering Associate Professor of Chemical and Biological Engineering ...
(Date:7/30/2014)... the potential effects of climate change on the ... the wrong factors, according to a new paper ... Society, University of Queensland, and other organizations. The ... is missing the point when it comes to ... climate change scientists focus on the "direct" threats ...
Breaking Biology News(10 mins):Hope for the overweight 2Engineering a protein to prevent brain damage from toxic agents 2Conservation scientists asking wrong questions on climate change impacts on wildlife 2
... This release is available in German . ... Zentrum Mnchen, the German Center for Neurodegenerative Diseases (DZNE) and ... have now discovered that lipid peroxides* play a specific physiological ... already established that accumulation of lipid peroxides* indicate cell stress. ...
... new study shows that male rats prenatally exposed to low ... to develop prostate inflammation and to go through puberty later ... of literature on atrazine, an herbicide predominantly used to control ... cane. Atrazine and its byproducts are known to be relatively ...
... a half billion years ago, Earth differed greatly from ... of gases in the atmosphere and the nature of ... consists of about 21 percent oxygen, the ancient atmosphere ... organisms. The complex eukaryotic life we are familiar with ...
Cached Biology News:Lipid peroxides -- more sophisticated than their reputation 2Atrazine causes prostate inflammation in male rats and delays puberty 2Ancient microbes responsible for breathing life into ocean 'deserts' 2Ancient microbes responsible for breathing life into ocean 'deserts' 3Ancient microbes responsible for breathing life into ocean 'deserts' 4
(Date:7/31/2014)... Nearly 20,000 medical professionals and healthcare leaders ... Clinical Lab Expo in Chicago ... diagnostic research and technology that will advance medicine and ... of Wednesday, July 30, more than 19,500 attendees had ... Lab Expo, with more than 10,000 of these attendees ...
(Date:7/31/2014)... the sound-processing part of the mouse brain in a ... trees in view. Their imaging technique allows zooming in ... and it enabled the team to watch brain cells ... results, which represent a step toward better understanding how ... the journal Neuron . , In the past, ...
(Date:7/31/2014)... Calif. , July 31, 2014 Rogne ... San Francisco Bay Area , has acquired a ... of Oxford,s commercialisation company. The potent anti-inflammatory activity of ... at the University of Oxford,s Sir William ... company, which has raised over $1.5 million in seed ...
(Date:7/31/2014)... 2014 Research and Markets has ... 2014-2018" report to their offering. ... study of the genetic material or genomes of an ... for the discovery and development of diagnostic and therapeutic ... conditions. Genomics is supported by three major product segments: ...
Breaking Biology Technology:Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 3New mapping approach lets scientists zoom in and out as the brain processes sound 2Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 2Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 3Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2
... New England Biolabs has expanded its line of PCR reagents ... Start DNA Polymerases. Optimized blends of Taq and ... robust amplification across a wide range of templates, including routine, ... Vent increases the fidelity and robustness of Taq , ...
... 2011 QPS Holdings, LLC, a leading full-service ... support preclinical and clinical research and development, announced ... Preclinical Sciences (CTPS) from Taiwan,s Development Center for ... a division of QPS-Taiwan. QPS is ...
... Inc., a pharmaceutical company developing orally-available broad spectrum ... $45 million Series F financing. New Leaf Venture ... investors Pappas Ventures and Morningside Group, as well ... Partners, Asset Management Company and Frazier Healthcare Ventures. ...
Cached Biology Technology:New England Biolabs Introduces OneTaq™ DNA Polymerase, a Robust Solution for Routine and Difficult PCR 2QPS Expands Into Toxicology Services With Acquisition of the Preclinical Unit of DCB Taiwan 2Chimerix Completes $45 Million Financing to Fund Further Development of Novel Antiviral Therapeutics CMX001 and CMX157 2Chimerix Completes $45 Million Financing to Fund Further Development of Novel Antiviral Therapeutics CMX001 and CMX157 3Chimerix Completes $45 Million Financing to Fund Further Development of Novel Antiviral Therapeutics CMX001 and CMX157 4
... contains sufficient materials for 1,464 array sample elements. ... , Blocker BSA in PBS (10X): ... HRP-conjugated: 10 g , BupH Phosphate ... Tween-20: 2 x 10 ml ampules , SuperSignal ...
... The Partec Cell Counter Analyser counts ... that the CCA detects only cells, so ... influence the results.,While passing through a 250 ... a fluorescence light signal which is used ...
... system, 100-120 V, is used for biolistic ... handheld device employs an adjustable helium pulse ... gold microcarriers from the inner wall of ... cells. This system has a 2 square-centimeter ...
... Results Laser Capture Microdissection (LCM) enables ... accuracy of molecular assays by starting with ... structures isolated from whole tissue or cytology ... 1996 at the National Institutes of Health ...
Biology Products: