Navigation Links
New technique helps researchers determine amino-acid charge

d. "Previous work has told us how the helices are oriented in the closed state."

A major problem in understanding the relationship between structure and function in proteins, and the impact that electrostatics have on them, Grosman said, is not knowing the protonation state of ionizable residues. Protons, he added, are so small that they cannot be detected even with X-ray crystallographic approaches.

For the study, Grosman and colleagues used protein engineering. They mutated each residue of the pore's lining with basic amino-acid residues, which can acquire a positive charge upon binding protons and become neutral upon releasing them.

"One thing is the proton affinity of an amino-acid residue when the amino acid is dissolved in a lot of water in, say, a glass beaker; another thing is the affinity for protons in the complex microenvironment presented by a membrane protein," Grosman said. "For the first time, we were able to measure proton-transfer events at the single-proton, single-amino-acid level, in real time. Chemists will be happy to see this."

In addition to providing an extensive set of proton-affinity values for basic residues, which differed greatly from those found in a "glass beaker," the findings also discounted a long-held theory that the rotation of the pore-lining helices underlie the mechanism of opening and closing of the nicotinic receptor ion channel. The data, Grosman said, indicate that such rotation is minimal, if any.


'"/>

Source:University of Illinois at Urbana-Champaign


Page: 1 2

Related biology news :

1. New lab technique identifies high levels of pathogens in therapy pool
2. Brain-mapping technique aids understanding of sleep, wakefulness
3. Study reveals new technique for fingerprinting environmental samples
4. Researchers pioneer new gene therapy technique using natural repair process
5. Newer imaging techniques may lead to over-treatment
6. Gene silencing technique offers new strategy for treating, curing disease
7. Mosaic mouse technique offers a powerful new tool to study diseases and genetics
8. Researchers devise new technique for creating human stem cells
9. New technique rapidly detects illness-causing bacteria
10. New bioinformatics technique for systematically analyzing key regions in DNA that help control gene activity
11. New technique may speed DNA analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New technique helps researchers determine amino acid charge

(Date:9/2/2014)... Yale Cancer Center researchers may have discovered a ... cells made vulnerable by deficient DNA repair. , The ... Reports . , The study, led by James ... Yale School of Medicine, found that cancer cells with ... their own genetic damage) were significantly more vulnerable to ...
(Date:9/2/2014)... digital distribution of media will have lower carbon ... when file sizes are large. , That,s the ... Journal of Industrial Ecology that looked at ... as PlayStation3. Researchers found that Blu-ray Discs delivered ... than game files downloaded over broadband Internet. For ...
(Date:9/2/2014)... 2, 2014 In a new study that could ... Florida campus of The Scripps Research Institute (TSRI) have ... unique manufacturing sites for molecules that can treat a ... as a reaction vessel and a disease-causing defect as ... cell," said TSRI Professor Matthew Disney.,"Because the treatment is ...
Breaking Biology News(10 mins):Sabotage as therapy: Aiming lupus antibodies at vulnerable cancer cells 2Scripps Florida scientists make diseased cells synthesize their own drug 2
... pathogens rather than kill them, and although they could be ... humans. A new study to be published in the online ... drugs have the potential to fight infection while avoiding the ... kill or stop the growth of pathogens, but antivirulence drugs ...
... Diego School of Medicine say that losing the ability to ... in early hominids, and may have directed the evolutionary emergence ... findings, published in this week,s early online edition of the ... among the first evidence of a novel link between cell ...
... York and Hull York Medical School has revealed important ... by age. Working with scientists at the Peninsula ... studied responses to stress in synapses -- neuronal connections. ... such as neuro-degeneration, resulting high energy forms of damaging ...
Cached Biology News:Can antivirulence drugs stop infections without causing resistance? 2Sexual selection by sugar molecule helped determine human origins 2Sexual selection by sugar molecule helped determine human origins 3Researchers study aging's effect on the brain 2
(Date:9/2/2014)... critical, yet often overlooked factor in the medical ... effects of aggregation on both radiofrequency heating and ... oxide nanoparticles (IONPs), including detailed analysis of the ... is shown to produce significant reductions in both ... new method to quantify and correlate these effects ...
(Date:9/2/2014)... including University of Oregon chemist Geraldine Richmond, have ... self-assembling, synthetic proteins called peptoid nanosheets that mimic ... -- detailed this week in a paper placed ... of the National Academy of Sciences -- ... the two-dimensional peptoid nanosheets that can be used ...
(Date:9/2/2014)... , Sept. 2, 2014  TransCelerate BioPharma Inc. ... recommended approach for protecting personal data in Clinical ... patients and others. The document describes an approach ... from CSRs and other related clinical trial documents, ... The goal of the TransCelerate CSR Redaction approach ...
(Date:9/2/2014)... Opertech Bio, Inc., a company ... effective approach to sensory evaluation, today announced the ... U.S. Patent and Trademark Office, encompassing high-throughput sensory ... “Opertech’s taste evaluation technology called Microtiter Operant Gustometer ... system for rapid characterization of taste sensory properties,” ...
Breaking Biology Technology:Accounting for biological aggregation in heating and imaging of magnetic nanoparticles 2UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique 2TransCelerate BioPharma Inc. Releases Recommended Approach for Protecting Personal Data in Clinical Study Reports 2TransCelerate BioPharma Inc. Releases Recommended Approach for Protecting Personal Data in Clinical Study Reports 3Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... Randomized Study Under Consideration, ROCKVILLE, Md., Nov. ... clinical-stage pharmaceutical company developing,therapeutics for the treatment of ... met the primary endpoint for the efficacy portion ... in combination with pemetrexed,(Alimta(R)) in non-small cell lung ...
... update on business and product pipeline, BOTHELL, ... Inc. (NASDAQ: OGXI ), a biopharmaceutical company ... that address,unmet needs in the treatment of cancer, ... and nine months ended September 30, 2008., ...
... 10 /Xinhua-PRNewswire/ -- Simcere Pharmaceutical,Group (NYSE: SCR ... generic,pharmaceuticals and innovative anti-cancer pharmaceuticals in the rapidly,growing ... Morgan,Stanley 7th Annual Asia Pacific Summit 2008. The ... on November 11-13, 2008., Simcere,s chief financial ...
Cached Biology Technology:EntreMed's MKC-1 Meets Primary Efficacy Endpoint in Non-Small Cell Lung Cancer Clinical Trial 2EntreMed's MKC-1 Meets Primary Efficacy Endpoint in Non-Small Cell Lung Cancer Clinical Trial 3OncoGenex Reports Third Quarter Financial Results 2OncoGenex Reports Third Quarter Financial Results 3OncoGenex Reports Third Quarter Financial Results 4OncoGenex Reports Third Quarter Financial Results 5OncoGenex Reports Third Quarter Financial Results 6OncoGenex Reports Third Quarter Financial Results 7OncoGenex Reports Third Quarter Financial Results 8OncoGenex Reports Third Quarter Financial Results 9Simcere Pharmaceutical Group to Participate in Morgan Stanley Seventh Annual Asia Pacific Summit 2008 2
...
solute carrier family 25, member 13 (citrin)...
... Ladder is suitable for sizing ... 16 kb by agarose gel ... be visualized by ethidium bromide ... detected on Southern blots by ...
Immunogen: Recombinant full length protein, corresponding to amino acids 1-435 of Human AP2M1. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products: