Navigation Links
New technique helps researchers determine amino-acid charge

Measurements of the ion-current through the open state of a membrane-protein's ion channel have allowed scientists at the University of Illinois at Urbana-Champaign to obtain a detailed picture of the effect of the protein microenvironment on the affinity of ionizable amino-acid residues for protons.

The findings, reported in the Dec. 15 issue of Nature, are expected to be welcome news for chemists and biophysicists, both experimentalists and theoreticians, because they have previously relied on theoretical estimations to predict protonation states -- whether an amino acid is charged or not. Appropriate experimental benchmarks also had been lacking.

All cells have membrane proteins that form channels to allow water and/or ions to pass through. Malfunctions have been linked to such problems as hypertension, abnormal insulin secretion, abnormal heart conditions and brain seizures. As a result, these membrane proteins are often targeted by drug treatments.

Previous approaches didn't provide sufficient resolution to let researchers accurately detect the association and dissociation of protons to and from individual amino-acid residues in real time.

Using the patch-clamp technique, the researchers were able to probe the electrostatic properties of the inner lining of the ion-channel's pore, and, from there, they inferred the rotational angle of the pore-lining alpha-helices in the open state.

In this case, researchers focused on the muscle nicotinic acetylcholine receptor, a membrane protein that mediates voluntary muscle contraction.

"Our paper has implications that are specific to this receptor, but many of the findings can be extended to several other membrane proteins," said Claudio Grosman, a professor of molecular and integrative physiology at Illinois.

"We are working with the open state of the ion channel, and we now know how the helices that line the pore are oriented. This was not known before," Grosman sai
'"/>

Source:University of Illinois at Urbana-Champaign


Page: 1 2

Related biology news :

1. New lab technique identifies high levels of pathogens in therapy pool
2. Brain-mapping technique aids understanding of sleep, wakefulness
3. Study reveals new technique for fingerprinting environmental samples
4. Researchers pioneer new gene therapy technique using natural repair process
5. Newer imaging techniques may lead to over-treatment
6. Gene silencing technique offers new strategy for treating, curing disease
7. Mosaic mouse technique offers a powerful new tool to study diseases and genetics
8. Researchers devise new technique for creating human stem cells
9. New technique rapidly detects illness-causing bacteria
10. New bioinformatics technique for systematically analyzing key regions in DNA that help control gene activity
11. New technique may speed DNA analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New technique helps researchers determine amino acid charge

(Date:7/11/2014)... Social animals often develop relationships with other group members ... In wild chacma baboons the strategy for grooming activities ... are just published in the scientific journal Biology ... of baboons is not practiced without ulterior motives. To ... for the individual, while grooming another individual can provide ...
(Date:7/11/2014)... 11, 2014 The increased risk of kidney injury ... resuscitation fluids reflects the mass of HES molecules, according ... official journal of the International Anesthesia Research Society (IARS). ... harmful effect of HES on cultured human renal proximal ... Christian Wunder and colleagues of University Hospital Wrzburg, Austria. ...
(Date:7/11/2014)... cannabinoid receptor type 1 can inhibit voltage-gated ... reduce neurotransmitter release. However, some scholars demonstrated ... extracellular Ca2+ influx and increase neurotransmitter release. ... Hospital Affiliated to Tongji Medical College, Huazhong ... whole cell voltage-clamp and calcium imaging in ...
Breaking Biology News(10 mins):Baboons groom early in the day to get benefits later 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... More than 20 percent of atheist scientists are spiritual, ... public marries spirituality and religion, the study found that spirituality ... with scientific discovery for "spiritual atheist" scientists. ... Sociology of Religion . Through in-depth interviews ...
... , A new study from Cambridge University has for ... a higher than average number of autistic traits. ... today in the Journal of Autism and Developmental ... of biological girls with gender incongruence that persists into ...
... Amsterdam, 5 May 2011 Elsevier, the ... products and services, announced today the launch ... The journal aims at reporting the most recent ... intelligent computation based on the principles of swarm ...
Cached Biology News:More than 20 percent of atheist scientists are spiritual 2Female-to-male transsexual people have more autistic traits 2Elsevier announces launch of Swarm and Evolutionary Computation 2
(Date:7/10/2014)... 2014 On July 9 Prime ... presented Russia,s first national "Industry" ... Company is developing a unique project called MabNext ... a number of innovative drugs based on monoclonal antibodies ... took place at the International Exhibition "Innoprom 2014" in ...
(Date:7/10/2014)... 10, 2014   Ceres, Inc . (Nasdaq: ... announced today financial results for the three months ended ... business. Ceres reported that the company and ... this growing season in Brazil, which concluded in June, ... sorghum evaluation areas for part of the growing season. ...
(Date:7/10/2014)... in the journal PNAS (Proceedings of the National ... day and sleep deprivation have a significant effect on ... at the best time of day to test for ... administering medicines effectively. , Researchers from the University of ... the links between sleep deprivation, body clock disruption and ...
(Date:7/10/2014)... can sit upside down forever. Nanophysics makes sure of that. ... article just published in Physical Review E . Knowledge ... spiders that seem to be able to sit still forever, ... many years. We will soon be able to buy smart ... foot. But the fact is, sooner or later the grip ...
Breaking Biology Technology:Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Time of day crucial to accurately test for diseases, new research finds 2Even geckos can lose their grip 2
... , ROCKAWAY, N.J., Dec. 14 ... Approach , published by the Infusion Nurses ... extravasation.(1) Anthracyclines are a group of chemotherapy medications including ... the treatment of various types of cancer since the early ...
... , WALTHAM, Mass., Dec. ... that it has signed a new, three-year master services ... and services that serve patients in approximately 100 countries. ... for translation of the company,s online content, which includes ...
... WALTHAM, Mass., Dec. 14 Decision Resources, one of the world,s ... that, owing to the launches of several new agents, the atrial ... in 2008 to $6.1 billion in 2018 in the United States, ... new Pharmacor report entitled Atrial Fibrillation finds that a ...
Cached Biology Technology:Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 2Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 3Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 4Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 5Multi-Billion Dollar Biotech Firm Chooses Lionbridge as Exclusive Translation Provider with Three-Year Master Services Agreement 2Multi-Billion Dollar Biotech Firm Chooses Lionbridge as Exclusive Translation Provider with Three-Year Master Services Agreement 3The Atrial Fibrillation Drug Market Will Increase More Than Sevenfold, from $790 Million in 2008 to $6.1 Billion in 2018 2
Detect and measure levels of mouse IgG2b antibody isotypes quickly and easily....
Fast, sensitive quantitation of Mouse IgM concentration....
Detect and measure levels of mouse IgG3 antibody isotypes quickly and easily....
Request Info...
Biology Products: