Navigation Links
New technique helps identify multiple DNA regulatory sites

Just as the printing press revolutionized the creation of reading matter, a "nano-printing" technique developed at MIT could enable the mass production of nano-devices currently built one at a time.

The most immediate candidate for this innovation is the DNA microarray, a nano-device used to diagnose and understand genetic illnesses such as Alzheimer's, viral illnesses such as AIDS, and certain types of cancer.

The ability to mass produce these complex devices would make DNA analysis as common and inexpensive as blood testing, and thus greatly accelerate efforts to discover the origins of disease.

The demand for ever-shrinking devices of ever-increasing complexity in areas from biomedicine to information technology has spurred several research efforts toward high-resolution, high-throughput nano-printing techniques. Professor Francesco Stellacci and graduate student Arum Amy Yu, both in the Department of Materials Science and Engineering, have developed a printing method that is unmatched in both information content per printing cycle and resolution. They achieved the latter using what Yu calls "nature's most efficient printing technique: the DNA/RNA information transfer."

In the new printing method, called Supramolecular Nano-Stamping (SuNS), single strands of DNA essentially self-assemble upon a surface to duplicate a nano-scale pattern made of their complementary DNA strands. The duplicates are identical to the master and can thus be used as masters themselves. This increases print output exponentially while enabling the reproduction of very complex nano-scale patterns.

One such pattern is found on a DNA microarray, a silicon or glass chip printed with up to 500,000 tiny dots. Each dot comprises multiple DNA molecules of known sequence, i.e. a piece of an individual's genetic code. Scientists use DNA microarrays to discover and analyze a person's DNA or messenger-RNA genetic code. This allows for, say, the early diagnosis of liver cancer, or the prediction of the chances that a couple will produce a child with a genetic disease.

Frequent, widespread use of these devices is hindered by the fact that producing them is a painstaking process that involves at least 400 printing steps and costs approximately $500 per microarray.

MIT's nano-printing method requires only three steps and could reduce the cost of each microarray to under $50. "This would completely revolutionize diagnostics," said Stellacci. With the ability to mass produce these devices and thus make DNA analysis routine, "we could know years in advance of cancer, hepatitis, or Alzheimer's."

Another benefit would be large-scale diagnostics that could provide useful information about disease. Take diabetes. "We don't know if it's genetic. The only way to find out is to test a lot of people," said Stellacci. "The more we test with microarrays, the more we know about illnesses, and the more we can detect them."

SuNS has applications beyond DNA microarrays. Materials both organic and inorganic (metal nanoparticles, for example) can be made to assemble along a pattern composed of DNA strands. This makes SuNS a versatile technology that could be used to produce other complex nano-devices currently manufactured slowly and expensively: micro- and nano-fluidics channels, single-electron transistors, optical biosensors and metallic wires, to name a few.

Stellacci recently received renewed funding from the Deshpande Center for Technological Innovation to continue work on SuNS. The work is also funded by the National Science Foundation.

A version of this article appeared in the May 18, 2005 issue of MIT Tech Talk (Volume 49, Number 28).


'"/>

Source:


Related biology news :

1. New lab technique identifies high levels of pathogens in therapy pool
2. Brain-mapping technique aids understanding of sleep, wakefulness
3. Study reveals new technique for fingerprinting environmental samples
4. Researchers pioneer new gene therapy technique using natural repair process
5. Newer imaging techniques may lead to over-treatment
6. Gene silencing technique offers new strategy for treating, curing disease
7. Mosaic mouse technique offers a powerful new tool to study diseases and genetics
8. Researchers devise new technique for creating human stem cells
9. New technique rapidly detects illness-causing bacteria
10. New bioinformatics technique for systematically analyzing key regions in DNA that help control gene activity
11. New technique may speed DNA analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/2/2016)... LONDON , June 2, 2016 ... has awarded the 44 million US Dollar project, ... Security Embossed Vehicle Plates including Personalization, Enrolment, and IT Infrastructure ... world leader in the production and implementation of Identity Management ... in January, however Decatur was selected ...
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
(Date:5/24/2016)... facilitates superior patient care by providing unparalleled technology to leaders of the medical imaging ... product recently added to the range of products distributed by Ampronix. Photo ... ... ... News ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... 27, 2016  Sequenom, Inc. (NASDAQ: SQNM ... lives through the development of innovative products and services, ... the United States denied its petition to ... of Sequenom,s U.S. Patent No. 6,258,540 (",540 Patent") are ... by the Supreme Court,s Mayo Collaborative Services v. Prometheus ...
(Date:6/27/2016)... , ... June 27, 2016 , ... Parallel 6 ... trials, announced today the Clinical Reach Virtual Patient Encounter CONSULT module which ... with the physician and clinical trial team. , Using the CONSULT module, patients and ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, former ... of the University of North Carolina Kenan-Flagler Business School effective June ... UNC Kenan-Flagler, with a focus on the school’s international efforts, leading classes and ...
(Date:6/27/2016)... June 27, 2016   Ginkgo Bioworks , a ... engineering, was today awarded as one of the ... the world,s most innovative companies. Ginkgo Bioworks is ... the real world in the nutrition, health and ... directly with customers including Fortune 500 companies to ...
Breaking Biology Technology: