Navigation Links
New technique boosts size of proteins that can be analyzed

Imagine you had to break a secret code, but you could see only part of the message. That's the kind of frustration researchers face when trying to identify proteins and characterize how those proteins are modified in cells by biological processes.

But now, Cornell researchers have extended a powerful technique to increase by fourfold the size of a protein that can be analyzed, to those containing more than 2,000 amino acids, up from about 500.

Called a "top-down" approach, the technique uses a mass spectrometer, which measures the masses of ions or charged particles. Researchers break up the protein into pieces and weigh both the masses of the whole protein and of the individual pieces. By matching the weight of the whole protein and its pieces with those of known protein sequences in a database, they can identify the protein. Any differences in mass with known proteins can help researchers also find where and how proteins have been modified in cells.

For example, if a section of a protein has an increased weight of 16, researchers can tell that the protein has been oxidized within that section, which means that an oxygen atom (with an atomic weight of 16) was added.

"When you isolate a protein from a mixture, your first problem is to know which one it is," said Fred McLafferty, Cornell's Peter J.W. Debye Professor Emeritus of Chemistry and Chemical Biology and senior author of the paper published in the Oct. 6 issue of the journal Science. "Mass spectrometry characterizes a protein by measuring the masses produced from it."

The new technique provides a far more efficient way to break down proteins inside the mass spectrometer -- sprayed, heated and then bashed with gas molecules -- to obtain their pieces for the mass analysis. They are sprayed in an electric field where they pick up charges and are vaporized as they pass through a heated capillary. Then they are hit by air molecules in low-energy collisions to keep the pro
'"/>

Source:Cornell University News Service


Page: 1 2

Related biology news :

1. New lab technique identifies high levels of pathogens in therapy pool
2. Brain-mapping technique aids understanding of sleep, wakefulness
3. Study reveals new technique for fingerprinting environmental samples
4. Researchers pioneer new gene therapy technique using natural repair process
5. Newer imaging techniques may lead to over-treatment
6. Gene silencing technique offers new strategy for treating, curing disease
7. Mosaic mouse technique offers a powerful new tool to study diseases and genetics
8. Researchers devise new technique for creating human stem cells
9. New technique rapidly detects illness-causing bacteria
10. New bioinformatics technique for systematically analyzing key regions in DNA that help control gene activity
11. New technique may speed DNA analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New technique boosts size proteins that can analyzed

(Date:8/29/2014)... of the Tibetan plateau -- the largest topographic anomaly ... both its profound effect on climate and its reflection ... GSA Bulletin , Katharine Huntington and colleagues employ a ... modern and fossil snail shells to investigate the uplift ... , Views range widely on the timing of surface ...
(Date:8/28/2014)... America (ESA) is pleased to announce the selection of ... Society. Honorary Membership acknowledges those who have served ESA ... the affairs of the Society that has reached an ... the ESA Governing Board and then voted on by ... the Awards Ceremony at Entomology 2014, ESA,s Annual Meeting ...
(Date:8/28/2014)... reports that an expansion of marine protected areas is ... functions. According to investigators from the Wildlife Conservation ... have focused on saving the largest numbers of species, ... key and difficult-to-replace ecological functions. , Many ... fish species that also are food for millions of ...
Breaking Biology News(10 mins):Snails tell of the rise and fall of the Tibetan Plateau 2Snails tell of the rise and fall of the Tibetan Plateau 3Snails tell of the rise and fall of the Tibetan Plateau 4Snails tell of the rise and fall of the Tibetan Plateau 5Snails tell of the rise and fall of the Tibetan Plateau 6Snails tell of the rise and fall of the Tibetan Plateau 7Fred Baxendale selected as Honorary Member of the Entomological Society of America 2Study finds marine protected areas inadequate for protecting fish and ocean ecology 2
... Howard Hughes Medical Institute (HHMI) scientists has peeled back some ... off genes selectively to control critical events of development. The ... the structure of Dicer, an enzyme that enables cells to ... in the January 13, 2005, issue of the journal Science ...
... Biobank, a visionary medical project aimed at improving the ... and many other serious conditions is launched this week ... three thousand people living in the south Manchester area ... before it goes nationwide later this year. , They ...
... at the University of Minnesota Medical School have discovered a ... have properties of primitive stem cells. , Umbilical ... that can only produce cells found in blood. The ... cells with the characteristics of more primitive stem cells that ...
Cached Biology News:Molecule does more than slice and dice RNA 2Molecule does more than slice and dice RNA 3Molecule does more than slice and dice RNA 4Multi-million pound UK Biobank underway 2Multi-million pound UK Biobank underway 3
(Date:8/29/2014)... DIEGO, Aug. 29, 2014  Pfenex Inc. (NYSE ... the development of high-value and difficult to manufacture ... update and reported financial results for the second ... the successful completion of our initial public offering, ... our biosimilar product candidates, derived from our proprietary ...
(Date:8/29/2014)... of the most promising technologies for future quantum circuits ... instead of electrons. First, it is necessary to be ... control their direction. Researchers around the world have made ... now scientists at the Niels Bohr Institute have succeeded ... at a time and in a particular direction. The ...
(Date:8/29/2014)... New York (PRWEB) August 29, 2014 ... valued at $5.6 billion in 2019. It is ... from 2013 to 2019, and was valued at ... report published by Transparency Market Research. , For ... visit: http://www.transparencymarketresearch.com/companion-diagnostics-market.html . , The research report, ...
(Date:8/29/2014)... According to a new market report published by Transparency ... valued at USD 3,754.6 million in 2012 and is estimated ... CAGR of 5.9% from 2013 to 2019. , Nearly half ... by bone and joint disorders, and this number is expected ... will continue to increase the demand for orthobiologics. Other factors ...
Breaking Biology Technology:Pfenex Reports Second Quarter 2014 Results and Provides Business Update 2Pfenex Reports Second Quarter 2014 Results and Provides Business Update 3Pfenex Reports Second Quarter 2014 Results and Provides Business Update 4Pfenex Reports Second Quarter 2014 Results and Provides Business Update 5Pfenex Reports Second Quarter 2014 Results and Provides Business Update 6Pfenex Reports Second Quarter 2014 Results and Provides Business Update 7Pfenex Reports Second Quarter 2014 Results and Provides Business Update 8Breakthrough in light sources for new quantum technology 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 2Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 3
... breath, sweat and skin have been used to detect humans ... of portable sensors for use in real-life situations, such as ... disasters in New Zealand and Japan. Published today, Monday ... , the study examined flumes of air to create a ...
... , Bochum,s physicists led by Prof. Dr. Hartmut Zabel ... for the first time experimentally. The behaviour of the ... research team at the RUB has now succeeded in ... resolution. Through their rotation of the magnetic moments, the ...
... Ore. -- A collaborative Oregon State University-University of Oregon ... Science Foundation grant in 2008, is moving into a ... The new NSF funding, awarded through its Centers ... Sustainable Materials Chemistry to expand research and development and ...
Cached Biology Technology:Scientists utilize breath and sweat to detect trapped humans 2Spin pumping effect proven for the first time 2Center for Sustainable Materials Chemistry gets Phase 2 NSF grant 2Center for Sustainable Materials Chemistry gets Phase 2 NSF grant 3Center for Sustainable Materials Chemistry gets Phase 2 NSF grant 4
Request Info...
... the 4300 System features Saga GT , ... SagaGT automates electrophoresis, lane finding, location of ... scoring. ,Saga GT is second generation ... was initially developed at the University of ...
... Polynucleotide Kinase (PNK) catalyzes the transfer ... the 5'-hydroxyl of single- and double-stranded ... enzyme also removes the 3'-phosphate from ... 3', 5'-diphosphates to form a 3'-hydroxyl ...
Request Info...
Biology Products: