Navigation Links
New method enables gene disruption in destructive fungal pathogen

ection of the new mutants, as well as a short partial target gene that integrates and disrupts genes in the pathogen's genome.

Richard Oliver, director of the Australian Centre for Necrotrophic Fungal Pathogens and professor of Molecular Plant Pathology at Murdoch University, Perth, commented: "The new disruption method looks highly promising as a tool for functional genomic studies. The authors looked at over 20 genes and were able to produce transformants and inactivated genes or knock-outs in each experiment. In most cases, the efficiency of gene disruption was 100 percent, which represents a considerable improvement over previously reported methods and makes large-scale functional analysis of individual genes feasible."

Yangrae Cho of VBI, lead scientist and author of the paper, said, "The high throughput system described in this study should allow for the systematic analysis of large sets of candidate genes in A. brassicicola, such as those encoding cell-wall-degrading enzymes and other genes of interest in pathogen-plant interactions."

The new gene disruption method may also find applications in the study of fungal pathogens that directly impact humans and human health. In addition to causing numerous plant diseases, Alternaria are involved in the development of such chronic airway diseases as asthma, allergy and chronic rhinosinusitis. Gene disruption methods could help in identifying molecules from the fungus that trigger inflammatory and other types of immune responses in humans. By understanding how fungi modulate immune responses in humans, new ways of developing therapeutics for these conditions could be identified.


'"/>

Source:Virginia Tech


Page: 1 2

Related biology news :

1. Color-blind method opens new doors in DNA sequencing
2. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
3. Studies reveal methods viruses use to sidestep immune system
4. An entropy-based gene selection method for cancer classification using microarray data
5. New methods of gene delivery using lasers
6. Breakthrough method in nanoparticle synthesis paves the way for new pharmaceutical and biomedical applications
7. Shift of weather patterns necessitates rethinking of reforestation methods
8. Scientists use manufacturing methods to reconstruct mastodon
9. Researchers develop promising new gene network analysis method
10. New method shows it is possible to grow bone for grafts within a patients body
11. Researchers develop new method for facile identification of proteins in bacterial cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New method enables gene disruption destructive fungal pathogen

(Date:4/18/2014)... Peru found that 28 percent of the batches ... Many pills released the active ingredient too slowly. ... had no active ingredient at all. , ... Georgia Institute of Technology developed a sophisticated approach ... drugs and then characterize their chemical composition. The ...
(Date:4/18/2014)... population predicted to reach 9 billion by 2050 ... considers human-altered landscapes such as farmland, according to ... that supports it might be an increasingly scarce ... of the land surface is directly affected by ... impacts such as climate change. But what if ...
(Date:4/18/2014)... Ancient DNA adds a twist to the story of ... be published April 21 in the journal Proceedings of ... the bones of chickens that lived 200-2300 years ago in ... domestic chickens may have looked far different from the chickens ... the traits we associate with modern domestic chickens -- such ...
Breaking Biology News(10 mins):Counterfeit contraceptives found in South America 2Counterfeit contraceptives found in South America 3Stanford researchers rethink 'natural' habitat for wildlife 2Stanford researchers rethink 'natural' habitat for wildlife 3Stanford researchers rethink 'natural' habitat for wildlife 4Ancient DNA offers clues to how barnyard chickens came to be 2
... , This release is available in ... thinks of herbal teas or valerian drops. However, that ... the Fraunhofer Institute for Molecular Biology and Applied Ecology ... to produce biopharmaceuticals. Those are proteins that, unlike many ...
... competitive realm of bacteria, cheaters must be kept in ... that in one bacterium, at least, bacterial cooperators can ... for dominance. "Even simple organisms such as ... Velicer, who with Ph.D. student Pauline Manhes has reported ...
... with rheumatoid arthritis are two times more likely to ... controls -- an association which was sustained even when ... controlled for, according to a study presented today at ... 15,766 patients with RA and 15,340 controls found that ...
Cached Biology News:Medicines from plants 2'Policing' stops cheaters from dominating groups of cooperative bacteria 2'Policing' stops cheaters from dominating groups of cooperative bacteria 3'Policing' stops cheaters from dominating groups of cooperative bacteria 4
(Date:1/15/2014)... , "The interface is the device," Nobel laureate ... properties to be found at the junctures where ... world of nanotechnology, the interfaces between layers of ... in such high-tech favorites as spintronics, high-temperature superconductors, ...
(Date:1/15/2014)... Cynvenio, a cancer diagnostics company focused on ... of tumor cells in the bloodstream, today announced that ... support application powered by CollabRx, Inc. , Available ... Therapy Finder will provide breast cancer patients and their ...
(Date:1/15/2014)... 2014 More than 5 million Americans ... in 3 seniors will die with Alzheimer’s or another ... figures have shocked many Americans into looking for ways ... these tragic age-related cognitive disorders. Jonathan Weisman, president of ...
(Date:1/15/2014)... 15, 2014 Freeslate, Inc ., ... announced that Lupin Limited, one of India’s top five ... Protégé PharmD System for high throughput solid form ... focused on a wide range of quality, affordable generic ...
Breaking Biology Technology:A deeper look at interfaces 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3
... the stress of the Second Lebanon War suffered fewer attacks ... focusing on feelings. This has been shown in a new ... the Technion-Israel Institute of Technology and Carmel Medical Center. "Because ... is important to uncover all the factors that impact the ...
... biggest Roman candle has got nothing on this. Using ... Earth or on a giant planet, Washington State University ... storing vast amounts of energy. "If you think about ... storage outside of nuclear energy," says Choong-Shik Yoo, a ...
... A major breakthrough in remote wave sensing by a ... for detecting hidden explosives, chemical, biological agents and illegal ... new, all-optical system, using terahertz (THz) wave technology, has ... it can "see through" clothing and packaging materials and ...
Cached Biology Technology:Can multiple sclerosis attacks be minimized in a war zone? 2A revolutionary breakthrough in terahertz remote sensing 2
Identify and characterize Phosphoinositide (PI(4,5)P2) binding...
... Moto Alpha unit with motorized heated lid and ... is useful for microarrays and chips. The four ... The sealing force of the motorized lid is ... give repeatable results. This interchangeable Moto Alpha unit ...
... Anti-Krueppel-like factor 15 (KLF15) KLF15 is ... found abundantly expressed in the liver, kidneys, ... NP_054798 Specificity: Polyclonal antibody produced in ... to a region of human KLF15 with ...
... For affinity chromatography, Affi-Prep protein ... (IgG), selectively remove IgG prior to ... adsorb immune complexes for antigen purification. ... region of immunoglobulins, especially IgG from ...
Biology Products: