Navigation Links
New method enables gene disruption in destructive fungal pathogen

Researchers at the Virginia Bioinformatics Institute (VBI) at Virginia Tech, Colorado State University, and Duke University Medical Center have developed a new method to determine gene function on a genome-wide scale in the fungal pathogen Alternaria brassicicola. This destructive fungus causes black spot disease, leading to considerable leaf loss in such economically important crops as canola, cabbage, and broccoli.

Genomic methods that allow the disruption of several thousand genes are needed because they allow high-throughput identification of genes and gene function. Such procedures are widely applicable and would be extremely useful in allowing scientists to investigate the key events that occur when a host interacts with a pathogen.

"The development of this protocol is timely as the genome sequence of A. brassicicola is scheduled for completion in 2006. We now have in our hands a versatile method that will allow us to dissect the pathogen's nucleotide sequence information and establish the function of many of the individual genes in this filamentous fungus," said Christopher Lawrence, associate professor at VBI, director of the project, and one of the authors of the study.

"A. brassicicola has consistently been used in studies with the weedy mustard plant Arabidopsis. The genome sequence of Arabidopsis was determined in 2001 and many methods are available to ascertain gene function in this plant," Lawrence said. "We now have a means to identify key fungal and plant genes that interact and ultimately lead to disease development or resistance. This is an extremely powerful research tool."

The generation of gene disruption mutants has been a limiting step for the analysis of gene function in most filamentous fungi. The new method takes advantage of a novel linear DNA construct that greatly improves the efficiency of targeted gene disruption. The DNA construct includes an antibiotic-resistance marker gene, which allows for easy sel ection of the new mutants, as well as a short partial target gene that integrates and disrupts genes in the pathogen's genome.

Richard Oliver, director of the Australian Centre for Necrotrophic Fungal Pathogens and professor of Molecular Plant Pathology at Murdoch University, Perth, commented: "The new disruption method looks highly promising as a tool for functional genomic studies. The authors looked at over 20 genes and were able to produce transformants and inactivated genes or knock-outs in each experiment. In most cases, the efficiency of gene disruption was 100 percent, which represents a considerable improvement over previously reported methods and makes large-scale functional analysis of individual genes feasible."

Yangrae Cho of VBI, lead scientist and author of the paper, said, "The high throughput system described in this study should allow for the systematic analysis of large sets of candidate genes in A. brassicicola, such as those encoding cell-wall-degrading enzymes and other genes of interest in pathogen-plant interactions."

The new gene disruption method may also find applications in the study of fungal pathogens that directly impact humans and human health. In addition to causing numerous plant diseases, Alternaria are involved in the development of such chronic airway diseases as asthma, allergy and chronic rhinosinusitis. Gene disruption methods could help in identifying molecules from the fungus that trigger inflammatory and other types of immune responses in humans. By understanding how fungi modulate immune responses in humans, new ways of developing therapeutics for these conditions could be identified.


'"/>

Source:Virginia Tech


Related biology news :

1. Color-blind method opens new doors in DNA sequencing
2. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
3. Studies reveal methods viruses use to sidestep immune system
4. An entropy-based gene selection method for cancer classification using microarray data
5. New methods of gene delivery using lasers
6. Breakthrough method in nanoparticle synthesis paves the way for new pharmaceutical and biomedical applications
7. Shift of weather patterns necessitates rethinking of reforestation methods
8. Scientists use manufacturing methods to reconstruct mastodon
9. Researchers develop promising new gene network analysis method
10. New method shows it is possible to grow bone for grafts within a patients body
11. Researchers develop new method for facile identification of proteins in bacterial cells
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/26/2016)... LONDON , April 26, 2016 /PRNewswire/ ... Systems, a product subsidiary of Infosys (NYSE: ... partnership to integrate the Onegini mobile security platform ... http://photos.prnewswire.com/prnh/20151104/283829LOGO ) The integration ... security to access and transact across channels. Using ...
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
(Date:3/23/2016)... 23, 2016 ... Gesichts- und Stimmerkennung mit Passwörtern     ... MESG ), ein führender Anbieter digitaler ... mit SpeechPro zusammenarbeitet, um erstmals dessen Biometrietechnologie ... die Möglichkeit angeboten, im Rahmen mobiler Apps ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... ... 2016 , ... FireflySci has been manufacturing quartz and glass ... the globe. Their cute firefly logo has been spreading to more and more ... calibration standards that never require recalibration. These revolutionary standards have changed many ...
(Date:5/25/2016)... ... May 25, 2016 , ... The Ankle ... plating options designed to address fractures of the distal tibia and fibula. This ... Acumed Ankle Plating System 3 is composed of seven plate families that span ...
(Date:5/25/2016)... ... 25, 2016 , ... Scientists at the University of Athens say they have ... be hampering the research that could lead to one good one. Surviving Mesothelioma has ... now. , The team evaluated 98 mesothelioma patients who got a ...
(Date:5/25/2016)... ... May 25, 2016 , ... Biohaven Pharmaceutical Holding Company ... granted the company’s orphan drug designation request covering BHV-4157 for the treatment of ... the FDA. , Spinocerebellar ataxia is a rare, debilitating neurodegenerative disorder that ...
Breaking Biology Technology: