Navigation Links
New method enables gene disruption in destructive fungal pathogen

Researchers at the Virginia Bioinformatics Institute (VBI) at Virginia Tech, Colorado State University, and Duke University Medical Center have developed a new method to determine gene function on a genome-wide scale in the fungal pathogen Alternaria brassicicola. This destructive fungus causes black spot disease, leading to considerable leaf loss in such economically important crops as canola, cabbage, and broccoli.

Genomic methods that allow the disruption of several thousand genes are needed because they allow high-throughput identification of genes and gene function. Such procedures are widely applicable and would be extremely useful in allowing scientists to investigate the key events that occur when a host interacts with a pathogen.

"The development of this protocol is timely as the genome sequence of A. brassicicola is scheduled for completion in 2006. We now have in our hands a versatile method that will allow us to dissect the pathogen's nucleotide sequence information and establish the function of many of the individual genes in this filamentous fungus," said Christopher Lawrence, associate professor at VBI, director of the project, and one of the authors of the study.

"A. brassicicola has consistently been used in studies with the weedy mustard plant Arabidopsis. The genome sequence of Arabidopsis was determined in 2001 and many methods are available to ascertain gene function in this plant," Lawrence said. "We now have a means to identify key fungal and plant genes that interact and ultimately lead to disease development or resistance. This is an extremely powerful research tool."

The generation of gene disruption mutants has been a limiting step for the analysis of gene function in most filamentous fungi. The new method takes advantage of a novel linear DNA construct that greatly improves the efficiency of targeted gene disruption. The DNA construct includes an antibiotic-resistance marker gene, which allows for easy sel
'"/>

Source:Virginia Tech


Page: 1 2

Related biology news :

1. Color-blind method opens new doors in DNA sequencing
2. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
3. Studies reveal methods viruses use to sidestep immune system
4. An entropy-based gene selection method for cancer classification using microarray data
5. New methods of gene delivery using lasers
6. Breakthrough method in nanoparticle synthesis paves the way for new pharmaceutical and biomedical applications
7. Shift of weather patterns necessitates rethinking of reforestation methods
8. Scientists use manufacturing methods to reconstruct mastodon
9. Researchers develop promising new gene network analysis method
10. New method shows it is possible to grow bone for grafts within a patients body
11. Researchers develop new method for facile identification of proteins in bacterial cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New method enables gene disruption destructive fungal pathogen

(Date:7/31/2014)... bigeye tuna movements in the northwestern Atlantic Ocean led ... Center at the University of Massachusetts Amherst, found among ... range with pronounced north-south movements from Georges Bank to ... off Cape Hatteras southwest of Bermuda for foraging. , ... study one of the most important commercial tuna species ...
(Date:7/31/2014)... Science Foundation (NSF), counters a widely-held scientific view ... instead that certain Arctic lakes store more greenhouse ... , The study, published this week in the ... which occur as permafrost thaws and creates surface ... what was previously frozen land into lakes. , ...
(Date:7/31/2014)... GAINESVILLE, Fla. --- By tracing nearly 3,000 genes ... moths, University of Florida scientists have created an ... to use large-scale, next-generation DNA sequencing. , ... more closely related to small moths than to ... how butterflies evolved. The study also found that ...
Breaking Biology News(10 mins):Study of bigeye tuna in Northwest Atlantic uses new tracking methods 2Study of bigeye tuna in Northwest Atlantic uses new tracking methods 3Certain Arctic lakes store more greenhouse gases than they release 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 3
... be complete without mosquitoes nipping at exposed skin. Or ... University team may help solve a problem that scientists ... Kun Yan Zhu, professor of entomology, and teammates ... Jianzhen Zhang, a visiting scientist from Shanxi University, China, ...
... Researchers writing in the open access journal BMC ... an adaptation in the blood of Eastern moles which ... moles, burrowing behavior. Kevin Campbell from the ... researchers to study the blood of three underground species ...
... has developed a "lab on a chip" device for ... white blood cells called T cells that are affected ... the test detects the types and levels of inflammatory ... Revzin,s team collaborated with UCLA electrical engineer Prof. Aydogan ...
Cached Biology News:K-State researchers find gene-silencing nanoparticles may put end to pesky summer pest 2Adaptation in mole blood aids tunnelling 2Novel microfluidic HIV test is quick and cheap 2
(Date:7/31/2014)... 31, 2014 Today BioSpace , the leading life sciences ... campaign spotlighting the life sciences community in Illinois , ... Kansas , Michigan , Minnesota ... Ohio and Wisconsin . ... of all job postings on BioSpace originating in this region in 2013. ...
(Date:7/31/2014)... ability to see through organs and even the entire ... as fine-grained cellular structures has been a long-time dream ... Press July 31st in the journal Cell ... methods for making opaque organs, bodies, and human tissue ... intact. The protocols could pave the way for a ...
(Date:7/31/2014)... According to a new market ... - Global Industry Analysis, Size, Share, Growth, Trends and ... USD 88.5 million in 2011, which is expected to ... CAGR of 53.8% from 2013 to 2023. ... Increasing demand for energy efficient displays ...
(Date:7/31/2014)... 2014 According to a new ... Medical Devices Market (Diagnostic Electrodes - ECG, EEG, EMG, ... Pacemakers, Defibrillators, Electrosurgical and Other Electrodes) - Global Industry ... 2019," the global electrodes market for medical devices was ... estimated to reach a market worth USD 1,451.2 million ...
Breaking Biology Technology:Midwest Biotech Leaders Featured On BioSpace Map 2See-through organs and bodies will accelerate biomedical discoveries 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 2Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 3Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 5Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 6
... BHRT ) a company committed to delivering ... treat heart failure and,cardiovascular diseases, announced the adoption ... -- Shareholders approved the election of eight ... in 2009 or until their successors are,duly elected ...
... July 30 DNA Heritage has recently,overcome patent claims ... use of DNA testing and databases that allow families ... DNA. The patent covers the,use of surnames and Y-chromosomes ... (UK IPO) has now rendered a formal opinion,stating that ...
... ROCKVILLE, Md., July 30 Stem cell ... Dr. Thomas Hazel will be,rejoining the company as ... Dr. Karl Johe, Neuralstem,s Chairman and Chief Science,Officer, ... ALS,(Amytrophic Lateral Sclerosis, or Lou Gehrig,s disease), and ...
Cached Biology Technology:Bioheart, Inc. Announces Results of Annual Meeting 2Bioheart, Inc. Announces Results of Annual Meeting 3Bioheart, Inc. Announces Results of Annual Meeting 4Bioheart, Inc. Announces Results of Annual Meeting 5DNA Heritage Success in Patent Battle Helps Keep Genealogy DNA Test Prices Low 2Neuralstem Fills Key Product Development Position 2
The Cellject Elecroporator is suitable for all cell types including embryo, mammalian, plant, yeast and bacterial. Its has highly user-freindly programing and can be used remotely, for example in a l...
Request Info...
... Virus Reverse Transcriptase (AMV RT) catalyzes the ... or RNA:DNA hybrids (1). It requires a ... RNA primers) as well as Mg2+ or ... H activity. Both nonionic detergents and sulfhydryl ...
Request Info...
Biology Products: