Navigation Links
New law for resolution allows unprecedented sharpness in fluorescence microscopy

Editor : Detailed Information with Figures (pdf download)

Max Planck researchers have succeeded in overcoming the law postulated by Ernst Abbe in 1873 for diffraction limited resolution in light microscopes. Stefan Hell and his co-workers have established a new law that promises unlimited resolution in fluorescence microscopy. Future applications range from the imaging of cell interiors to the measuring of lithographic structures in microchip manufacturing, and substantial improvements in the quantification of the reaction kinetics of organic molecules (Phys. Rev. Lett., April 15 and Phys. Rev. Lett. May 6).

Ever since its invention in the 17th century, the light microscope has served as a key instrument to the advancement of knowledge. For biology, this statement is true even today, since focused light is the only way to examine living cells non-invasively. However, because of the wave nature of light, focused light is subject to diffraction. As early as 1873, Ernst Abbe recognized that this fact imposes an absolute limit on resolution in microscopy. Abbe, whose 100th year death anniversary was in February, captured this limit in a formula, which states that objects at distances that are smaller than a certain limit cannot be separated in a light microscope. This resolution limit can be largely ascribed to the fact that light cannot be focused to a smaller spot due to diffraction. It seemed impossible to image details smaller than a distance where delta d is equal to 200nm using conventional lenses and visible light.

For a long time, Abbe's law was regarded as being insurmountable. For higher resolution, the textbook knowledge was that a complicated and costly electron or scanning tunneling microscope would be required. However, over the past few years, researchers from the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany, have developed wi
'"/>

Source:Max-Planck-Gesellschaft


Page: 1 2 3

Related biology news :

1. High resolution snapshots detail dynamics of a cocaine antibody
2. High-resolution light microscope reveals the fundamental mechanisms of nerve communication
3. Recombination protein dynamics observed with single monomer resolution
4. With record resolution and sensitivity, tool images how life organizes in a cell membrane
5. Researchers find how protein allows insects to detect and respond to pheromones
6. Enzyme allows B cells to resist death, leading to leukemia
7. Tiny scaffolding allows stem cells to become working fat cells
8. Looks matter to female barn swallows
9. Stolen gene allows insect virus to enter cells
10. Researchers identify molecular anchor that allows bacterial invasion of central nervous system
11. Algal protein in worm neurons allows remote control of behavior by light
Post Your Comments:
(Date:10/18/2014)... suspected genetic conditions, a certain type of exome sequencing ... than traditional molecular diagnostic methods, according to a study ... released to coincide with the American Society of Human ... protein­coding region of the genome (the complete set of ... organism), has been rapidly applied in research settings and ...
(Date:10/17/2014)... release is available in German . ... of sperms every day in order to reproduce? And why ... the topic of the latest issue of the research journal ... The evolutionary biologist Steven Ramm from Bielefeld University Bielefeld has ... is not unusual for a female to copulate with several ...
(Date:10/17/2014)... To date, antidotes ... doctors are often limited to supportive therapy such as induced ... of drugs involved. So what can be done if a ... professor Jean-Christophe Leroux from the Institute of Pharmaceutical Sciences at ... "The task was to develop an agent that could eliminate ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Sperm wars 2Emergency aid for overdoses 2Emergency aid for overdoses 3
... be associated with lower blood pressure (BP), and the diets ... such as diet, body weight, physical activity and alcohol intake ... modifications have been shown to be effective for preventing and ... 32 studies published from 1900 to 2013 in which participants ...
... are revealing how microbes living on floating pieces of ... potential harm they pose to invertebrates, humans and other ... into the largely unexplored world of the "Plastisphere" ... plastic that was first discovered last year. ...
... Geosphere postings cover using traditional geochemistry with ... Belt; an investigation of mafic rock samples from a ... southeastern Colorado Plateau of New Mexico and Arizona; a ... and micro-analytical techniques; and an addition to the "The ...
Cached Biology News:AGU: Uncovering the secret world of the Plastisphere 2Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 2Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 3Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 4Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 5Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 6
(Date:10/22/2014)... Oct. 22, 2014   Synthetic Biologics, Inc. ... therapies for serious infections and diseases, announced today that ... Notice of Allowance for a composition of matter patent ... difficile program, SYN-004. This is Synthetic Biologics, first ... U.S. and adds to the Company,s extensive C. ...
(Date:10/22/2014)... and HONG KONG , Oct. ... disease therapeutics enterprise, announced today that rare disease expert ... as vice president, research. Dr. McKew brings more than ... leadership positions at the National Institutes of Health, Wyeth ... by Wyeth). Dr. McKew will lead aTyr,s efforts to expand ...
(Date:10/20/2014)... 20, 2014 Asterias Biotherapeutics, Inc. (NYSE ... signed a Notice of Grant Award (NGA) with ... October 1, 2014.  The NGA provides for the ... release of additional grant funds pursuant to the ... clinical development of Asterias, product, AST-OPC1. The grant ...
(Date:10/20/2014)... BOSTON , Oct. 20, 2014 ... commercialization company tackling big healthcare problems, announced today ... investment round, with participation from Invesco Perpetual, a ... be used to drive PureTech,s existing pipeline forward ... technologies. "PureTech has the scientific creativity to really ...
Breaking Biology Technology:Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 4aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6
... fewer than a thousand atoms, the electronic devices on semiconductor ... characterize them. Now for the first time, engineers have demonstrated ... them atom-by-atom. , ,In a study published in the Aug. ... a UW-Madison professor of electrical and computer engineering, and Keith ...
... - Gov. Jim Doyle on Monday expressed hope for ... Leader Bill Frist's recent decision to support removal of ... ,Doyle made his comments at the groundbreaking of the ... praised Wisconsin's medical development scene. , ,In remarks to ...
... Wis. - Esker Software , a provider of ... of a new mail house at its U.S. headquarters in ... Esker on Demand service. , ,The new mail processing ... in Lyon, France and its strategic partners in the U.S. ...
Cached Biology Technology:Engineers chart semiconductors on the scale of atoms 2Engineers chart semiconductors on the scale of atoms 3Engineers chart semiconductors on the scale of atoms 4Doyle praises Frist's new stem cell research position 2Doyle praises Frist's new stem cell research position 3
... The simple three step procedure (PCR, Exonuclease ... completed in one day. The typical cloning ... 80%. , Features and Benefits: , , ... into any expression vector , High Cloning ...
Designed for use with all 96-well SPRI reagent kits. Used with round bottom 96-well microplates, and 96-well thermal cycling plates....
... be used with all 96-well ... stacking of covered plates ... with automated systems ... are available for use with ...
... This CLS number is ... to easily match Cornings product ... yet, please order under the ... contact customer service for assistance. ...
Biology Products: