Navigation Links
New law for resolution allows unprecedented sharpness in fluorescence microscopy

Editor : Detailed Information with Figures (pdf download)

Max Planck researchers have succeeded in overcoming the law postulated by Ernst Abbe in 1873 for diffraction limited resolution in light microscopes. Stefan Hell and his co-workers have established a new law that promises unlimited resolution in fluorescence microscopy. Future applications range from the imaging of cell interiors to the measuring of lithographic structures in microchip manufacturing, and substantial improvements in the quantification of the reaction kinetics of organic molecules (Phys. Rev. Lett., April 15 and Phys. Rev. Lett. May 6).

Ever since its invention in the 17th century, the light microscope has served as a key instrument to the advancement of knowledge. For biology, this statement is true even today, since focused light is the only way to examine living cells non-invasively. However, because of the wave nature of light, focused light is subject to diffraction. As early as 1873, Ernst Abbe recognized that this fact imposes an absolute limit on resolution in microscopy. Abbe, whose 100th year death anniversary was in February, captured this limit in a formula, which states that objects at distances that are smaller than a certain limit cannot be separated in a light microscope. This resolution limit can be largely ascribed to the fact that light cannot be focused to a smaller spot due to diffraction. It seemed impossible to image details smaller than a distance where delta d is equal to 200nm using conventional lenses and visible light.

For a long time, Abbe's law was regarded as being insurmountable. For higher resolution, the textbook knowledge was that a complicated and costly electron or scanning tunneling microscope would be required. However, over the past few years, researchers from the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany, have developed wi
'"/>

Source:Max-Planck-Gesellschaft


Page: 1 2 3

Related biology news :

1. High resolution snapshots detail dynamics of a cocaine antibody
2. High-resolution light microscope reveals the fundamental mechanisms of nerve communication
3. Recombination protein dynamics observed with single monomer resolution
4. With record resolution and sensitivity, tool images how life organizes in a cell membrane
5. Researchers find how protein allows insects to detect and respond to pheromones
6. Enzyme allows B cells to resist death, leading to leukemia
7. Tiny scaffolding allows stem cells to become working fat cells
8. Looks matter to female barn swallows
9. Stolen gene allows insect virus to enter cells
10. Researchers identify molecular anchor that allows bacterial invasion of central nervous system
11. Algal protein in worm neurons allows remote control of behavior by light
Post Your Comments:
(Date:6/15/2015)... -- A new report [ 1 ... US consumers using mobile banking applications want their mobile devices ... voiceprint, instead of having to prove who they are with ... Identity   -   The Fusion of Financial Services, Mobile ... primary channel used by Gen X and Gen Y to ...
(Date:6/11/2015)... , June 11, 2015 Daon, ... announced today that its IdentityX Mobile Authentication Platform ... IDentity Online) Alliance is an industry consortium launched ... standards for simpler, stronger authentication.  In order to ... pass a rigorous series of tests that measure ...
(Date:6/9/2015)... , June 09, 2015 Research ... addition of the "Gesture Recognition & Touchless ... (Consumer Electronics, Automotive, & Others), Product (Biometric & ... to 2020" report to their offering. ... market is expected to reach $ 23.55 Billion ...
Breaking Biology News(10 mins):One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 2One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 3One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 4Daon's IdentityX Mobile Authentication Platform One of First Products to be Certified by FIDO Alliance 2Global Gesture Recognition & Touchless Sensing (Technology, Application & Products) Market 2015-2020 2
... , Oct. 21, 2013   Arcturus Therapeutics, Inc. , an industry ... today announced it has secured $5 million in a Series A ... Canada , Japan and ... http://photos.prnewswire.com/prnh/20130531/MM24393LOGO ) The Series A funding, which comprised ...
... MA In a biological quirk that promises to ... potentially treating Fragile X syndrome, scientists at the University ... out a gene important for messenger RNA (mRNA) translation ... in a mouse model of a prevalent human neurological ...
... Jude Children,s Research Hospital scientists has linked an inherited ... developing a pediatric acute lymphoblastic leukemia (ALL) subtype that ... today in the online edition of the scientific journal ... found in the GATA3 gene. Researchers reported the high-risk ...
Cached Biology News:Arcturus Therapeutics Raises $5 Million in Series A Funding to Maintain its Momentum in Nominating Lead Clinical Candidate in 2014 2Arcturus Therapeutics Raises $5 Million in Series A Funding to Maintain its Momentum in Nominating Lead Clinical Candidate in 2014 32 genetic wrongs make a biochemical right 22 genetic wrongs make a biochemical right 32 genetic wrongs make a biochemical right 4Inherited gene variation tied to high-risk pediatric leukemia and greater risk of relapse 2Inherited gene variation tied to high-risk pediatric leukemia and greater risk of relapse 3
(Date:7/1/2015)... July 1, 2015  Axovant Sciences Ltd. (NYSE: ... the treatment of dementia, today announced that the ... 22 at 5:45 p.m. EDT following its presentation ... International Conference 2015 (AAIC). The ... , Dr. Lawrence Friedhoff , Chief Development ...
(Date:7/1/2015)... Indianapolis, IN (PRWEB) , ... July 01, 2015 , ... ... the 2015 BIO International Convention in Philadelphia, PA. , The presentation took ... milestones and outlined the plans for the development of APX3330 for the treatment of ...
(Date:6/30/2015)... ... June 30, 2015 , ... Students and faculty from Beijing ... half of “Integrative Biotechnology,” an applied biotechnology course team-taught by faculty members from ... combines students from both universities and is taught on both continents. , “Our ...
(Date:6/30/2015)... , June 30, 2015  Juniper Pharmaceuticals, ... focused on developing therapeutics that address unmet medical ... George Elston will present at the ... Wednesday, July 8, 2015Time: , 4:45 PM EDTLocation: ... (live & archive): , www.juniperpharma.com, under  ,Investor, ...
Breaking Biology Technology:Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Apex Therapeutics Presented Company Overview at This Year’s BIO International Convention in Philadelphia, PA 2Rivier University and Beijing City University Academic and Cultural Exchanges Begin 2Juniper Pharmaceuticals to Present at Cantor Fitzgerald Inaugural Healthcare Conference on July 8, 2015 2
... ARUP Laboratories, pathologist, Ronald L. Weiss, MD, MBA, ... of American Pathologists at CAP ,11, the organization,s annual ... the CAP, the award honors members "who have made ... the specialty of pathology." (Logo: http://photos.prnewswire.com/prnh/20110915/SF69370LOGO ...
... BEACH, Fla., Nov. 9, 2011 Biotech developer Divine ... which has been expanding the topical hair-growth segment, is ... system, Polaris API Solution 9, to be supplied by ... With well-established materials like minoxidil sulfate, finasteride, alfatradiol, ...
... Nov. 9, 2011 Third Quarter Highlights ... Awarded $5.7 million contract from Department of Defense (DoD) for ... awarded PharmAthene 50% net profit split on worldwide sales of ... million in net profits Completed 1500L commercial scale-up and ...
Cached Biology Technology:ARUP Laboratories' Pathologist, Ronald L. Weiss, MD, Honored With Lifetime Achievement Award for Contributions to Patient Care and Pathology 2Divine Skin Rolls Out Polaris API Solution 9: A New Technology for Physicians 2Divine Skin Rolls Out Polaris API Solution 9: A New Technology for Physicians 3PharmAthene Reports Third Quarter 2011 Financial and Operating Results 2PharmAthene Reports Third Quarter 2011 Financial and Operating Results 3PharmAthene Reports Third Quarter 2011 Financial and Operating Results 4PharmAthene Reports Third Quarter 2011 Financial and Operating Results 5PharmAthene Reports Third Quarter 2011 Financial and Operating Results 6PharmAthene Reports Third Quarter 2011 Financial and Operating Results 7PharmAthene Reports Third Quarter 2011 Financial and Operating Results 8
Mouse monoclonal antibody raised against a partial recombinant ACTN4. NCBI Entrez Gene ID = ACTN4...
Rabbit polyclonal antibody to GluR2...
... can be used to test the crossreactivity ... sera that are commonly used as blocking ... spots for 19 different protein preparations on ... crossreactivity and species specificity, QC of purified ...
... array sample elements. , ,Includes: , DiscoverLight ... BSA in PBS (10X): 50 ml , ... , BupH Phosphate Buffered Saline: 8 packs ... ml ampules , SuperSignal West Pico Luminol Enhancer: 2 ...
Biology Products: