Navigation Links
New lab technique churns out fungus' potential cancer fighter

For the first time, researchers have developed a way to synthesize a cancer-killing compound called rasfonin in enough quantity to learn how it works.

Derived from a fungus discovered clinging to the walls of a New Zealand cave, the chemical tricks certain cancer cells into suicide while leaving healthy cells untouched.

"In 2000, scientists in Japan discovered that this compound might have some tremendous potential as a prototype anticancer agent, but no one has been able to study or develop it because it's so hard to get enough of it from natural sources," says Robert K. Boeckman, professor of chemistry.

"You either grow the fungus that makes it, or you go through a complicated chemical synthesis process that still yields only a minute amount," he says. "Now, after five years of effort, we've worked out a process that lets researchers finally produce enough rasfonin to really start investigating how it functions, and how we might harness it to fight cancer."

In 2000, researchers from Chiba University in Japan and the University of Tokyo simultaneously discovered a compound in certain fungi that selectively destroyed cells depending upon a gene called ras--one of the first known cancer-causing genes. They had found rasfonin, a compound that seemed tailor-made to knock out ras-dependent cancers like pancreatic cancer.

After six years, however, rasfonin's secrets remain a mystery because researchers can't make enough of it to carry out tests.

To bring about a new drug, organic chemists must produce a new chemical in enough quantity to test it under many different circumstances to tease out its modus operandi. Until now, no method existed to generate rasfonin, aside from growing more fungus--a time-consuming and terribly inefficient method.Boeckman, the Marshall D. Gates, Jr. Professor of Chemistry at the University of Rochester, has now revealed a process that produces 67 times more rasfonin than any previous method. For the first time, scientists can obtain enough rasfonin to conduct proper biological tests on it.

"At a guess, I'd say that rasfonin itself will not be the final compound that might come to market," says Boeckman. "But we need to figure out how it works, how it triggers the cancer cell to shut itself down. The key is to find exactly what buttons rasfonin is pushing, and then figure out if there's a way we can safely and more simply push those same buttons. But we couldn't do that until we have enough to test."

Even Boeckman's simplified process is notably complex, employing sophisticated organic reactions. Instead of the original method's 23 steps, Boeckman's has just 16--but finding them took five years of his team's hard work, skill and intuition.

Boeckman's paper, published in the Aug. 30 issue of the Journal of the American Chemical Society, outlines the sequence of steps showing how Boeckman's group inserted, removed, or altered the three-dimensional and chemical structure of their compound until they produced complete rasfonin. Diagrams of the complete process are available on the Web at pubs.acs.org.

"Very soon, researchers should be able to scale up this process rather easily to whatever volume they need," says Boeckman. "It may be a long road to a possible treatment, but at least we're now past the first hurdle."


'"/>

Source:University of Rochester


Related biology news :

1. New lab technique identifies high levels of pathogens in therapy pool
2. Brain-mapping technique aids understanding of sleep, wakefulness
3. Study reveals new technique for fingerprinting environmental samples
4. Researchers pioneer new gene therapy technique using natural repair process
5. Newer imaging techniques may lead to over-treatment
6. Gene silencing technique offers new strategy for treating, curing disease
7. Mosaic mouse technique offers a powerful new tool to study diseases and genetics
8. Researchers devise new technique for creating human stem cells
9. New technique rapidly detects illness-causing bacteria
10. New bioinformatics technique for systematically analyzing key regions in DNA that help control gene activity
11. New technique may speed DNA analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/21/2016)... Columbia , June 21, 2016 ... to the new role of principal product architect ... named the director of customer development. Both will ... chief technical officer. The moves reflect NuData,s strategic ... in response to high customer demand and customer ...
(Date:6/16/2016)... , June 16, 2016 ... size is expected to reach USD 1.83 billion ... Grand View Research, Inc. Technological proliferation and increasing ... applications are expected to drive the market growth. ... , The development of advanced multimodal ...
(Date:6/9/2016)... ISTANBUL , June 9, 2016  Perkotek an innovation leader in attendance control ... to seamlessly log work hours, for employers to make sure the right employees are ... Logo - http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
Breaking Biology News(10 mins):
(Date:12/6/2016)... 2016 The American Botanical Council (ABC) ... arnica ( Arnica montana ) through ABC,s Adopt-an-Herb ... HerbMedPro database, a comprehensive, interactive online tool ... research data on the uses and health effects ... Naturopathica, a wellness company with healing arts centers, ...
(Date:12/6/2016)... NY (PRWEB) , ... December 06, 2016 , ... ... integrator of custom industrial automation and IT solutions, today announced the company has ... has reliably delivered professionally executed automation and control systems integration services to leading ...
(Date:12/6/2016)... Zimmer Biomet Holdings, Inc. (NYSE and ... terms of its previously-announced cash tender offers (the ... (excluding accrued and unpaid interest to, but not ... and expenses related to the Offers) (the "Maximum ... the table below (collectively, the "Notes"). The terms ...
(Date:12/5/2016)... Axovant Sciences Ltd. (NYSE: AXON ... treatment of dementia, today announced that data on the ... disease will be presented at the 2016 Clinical Trials ... 2016 in San Diego . Intepirdine ... and complex measures of activities of daily living (ADLs) ...
Breaking Biology Technology: