Navigation Links
New genes implicated in rheumatoid arthritis

Researchers continue to search for genetic clues into rheumatoid arthritis (RA), a chronic inflammatory joint disease. While its specific cause is not yet known, RA has been linked to an inherited susceptibility. Interestingly, despite its strong genetic component, RA's occurrence among siblings seems to be random.

In the quest to identify disease-specific gene expression profiles in patients with RA, researchers at the University of Michigan Medical Center turned to an ideal population: genetically identical, disease-discordant twins. The July issue of Arthritis & Rheumatism (http://www.interscience.wiley.com/journal/arthritis) highlights the results of their state-of-the-art genetic analysis.

Increasing evidence over the past several years indicates that B-lymphocytes play a central role in RA's development. In this study, microarray analysis was applied to lymphoblastoid B cell lines (LCLs) from 11 pairs of monozygotic twins, all with one healthy and one RA-affected twin. A revolutionary DNA technology, microarray can be used to not only compare gene expression in two different tissue samples, but to examine the expression of thousands of genes at once. The researchers extracted complementary DNA from the cells of every twin, labelled samples with fluorescent dye to distinguish RA cells from disease-free cells, and hybridized each on a 20,000-gene chip. Then, using immunohistochemistry and real-time polymerase chain reaction, they confirmed the expression of the most significantly over-expressed genes in synovial tissues. In addition, they compared gene expression in synovial tissue of the RA patients with gene expression in synovial tissue of patients with osteoarthritis (OA).

Between the disease-discorda nt twins, minor yet measurable differences were detected in the expression of 1,163 transcripts, representing 827 uniquely named genes. Of this total, 3 genes were significantly over-expressed in the cells of RA patients relative to their healthy co-twins. The most significantly over-expressed gene was laeverin, a newly discovered enzyme that works to degrade proteins. The second most significantly over-expressed gene was 11ß-hydroxysteroid dehydrogenase type 2 (11ß-HSD2), a steroid pathway enzyme linked to inflammation and bone erosion. This gene was also found over-expressed in the synovial tissue of OA patients. The third most significantly over-expressed gene was cysteine-rich, angiogenic inducer 61 (Cyr61), well-established for its role in the formation of new blood vessels.

"Our findings provide the first evidence that laeverin is abundantly expressed in synovial tissue," notes the study's leading author, Joseph Holoshitz, M.D. "11ß-HSD2 and Cyr61 have not previously been directly implicated in RA," he adds. Uncovering 3 new genes with a clear abundance in RA, this study supports the promise of microarray analysis to not only provide further insights into the genetic components of this inflammatory disease, but also to help identify candidates for therapeutic intervention.


'"/>

Source:John Wiley & Sons, Inc.


Related biology news :

1. Newly-discovered class of genes determines ?and restricts ?stem cell fate
2. Inexpensive, mass-produced genes core of synthetic biology advances at UH
3. First atlas of key brain genes could speed research on cancer, neurological diseases
4. U-M scientists find genes that control growth of common skin cancer
5. Researchers find missing genes of ancient organism
6. Scientists document complex genomic events leading to the birth of new genes
7. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
8. Advances in the characterisation of the oyster mushroom genes
9. Researchers find new genes necessary to make embryo
10. Protein helps regulate the genes of embryonic stem cells
11. Compounds in plastic packaging act as environmental estrogens altering breast genes
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/9/2016)... , UAE, May 9, 2016 ... when it comes to expanding freedom for high net ... Even in today,s globally connected world, there is ... conferencing system could ever duplicate sealing your deal with ... obtaining second passports by taking advantage of citizenship via ...
(Date:4/26/2016)... BANGALORE, India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a ... ), and Onegini today announced a partnership to ... banking solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... banks to provide their customers enhanced security to ...
(Date:4/15/2016)... April 15, 2016 Research ... Gait Biometrics Market 2016-2020,"  report to their offering.  ... ) , ,The global gait biometrics market is ... during the period 2016-2020. Gait analysis ... can be used to compute factors that are ...
Breaking Biology News(10 mins):
(Date:5/27/2016)... ... May 27, 2016 , ... Doctors in Italy, Japan, the UK and the ... associated protein (BAP1) gene and its link to malignant mesothelioma. Surviving Mesothelioma has just ... the full article now. , The studies analyzed for the new report included ...
(Date:5/27/2016)... , May 27, 2016 At present, ... playing in this space know that volatility is what makes ... companies on ActiveWallSt.com: Synta Pharmaceuticals Corp. (NASDAQ: SNTA ... Inc. (NASDAQ: LPTN ), and Heat Biologics Inc. ... access to the technical alerts for these stocks at: ...
(Date:5/26/2016)... Jersey and READING, England ... Indegene ( http://www.indegene.com ), a leading global ... life science, pharmaceutical and healthcare organisations and TranScrip ... innovative scientific support throughout the product lifecycle, today ... the launch of IntraScience.      (Logo: ...
(Date:5/26/2016)... , May 26, 2016 Despite the ... value in this space. Today,s pre-market research on ActiveWallSt.com directs ... Health Inc. (NASDAQ: RDUS ), Cerus Corp. (NASDAQ: ... ARWR ), and Five Prime Therapeutics Inc. (NASDAQ: ... briefings at: http://www.activewallst.com/ On ...
Breaking Biology Technology: