Navigation Links
New Technique for Tracking Gene Regulators

rtain which of these genes CREB regulates by more traditional methods, evaluating one gene at a time, would be too labor-intensive, expensive, and take a very long time. Scientists have been working on short cuts, but all so far have limits. For example, in one recent technique, scientists mix the regulator protein of interest (let’s say, CREB) with the entire genome of a cell and let it bind. Then they fragment the genome into smaller pieces, 500 to 1,500 nucleotide bases long. Using antibodies that specifically recognize and bind to CREB, they then isolate the pieces that have CREB attached, and wash all the others away. Scientists can use traditional gene-sequencing methods to decipher the sequence of A, T, G, and C on these fragments and then locate their original positions on the genome, but it is a slow and somewhat expensive process. Other methods, such as matching the pieces to their compliments on microarrays, are limited by the size of genome they can analyze. Dunn’s team has come up with a technique to determine the positions of these 500-to-1,500-base-long pieces on an entire genome ?even one as large as the human genome ?relatively quickly and in very large numbers. After isolating the CREB-bound fragments, they release the CREB and then cut the DNA with a “restriction?enzyme that recognizes a particular nucleotide base sequence, CATG. Further manipulation of the cut ends allows them to isolate 20-base-long-fragments, or “tags,?in each direction from these cut sites. This gives the scientists a large number of very short DNA “tag?sequences, which all have the same start sequence of CATG plus 16 additional unknown bases. The scientists then “glue?together the tags into chains and sequence them. While small in length, the tags are large enough to allow computer searches to locate their specific positions on the complete genome in a manner that is analogous to a laser scanner reading barcodes on items in the grocery store. In this case the inventory isn’t
'"/>

Source:Brookhaven National Laboratory


Page: 1 2 3

Related biology news :

1. Live Recombinant Adenovirus Vaccine Technique Explored
2. Technique may allow cancer patients to freeze eggs, preserving fertility before starting treatment
3. Researchers Find New Technique To Identify Fetal Genetic Material From Amniotic Fluid
4. Techniques available to detect soil that inhibits destructive soybean pest
5. Technique offers new view of dynamic biological landscape
6. Technique makes it easier to see mouse embryo in all its glory
7. Technique speeds up detecting, treating wound bacteria
8. Technique monitors thousands of molecules simultaneously
9. Tracking the memory trace
10. Tracking sperm whales and jumbo squid
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Technique for Tracking Gene Regulators

(Date:4/17/2014)... Our eyes not ... with a continuous stream of information about our own movements. ... a car the world glides by us and leaves ... our brain calculates self-motion from this "optic flow". This way, ... during our own movements. Together with biologists from the University ...
(Date:4/17/2014)... Rajendra Singh Thursday as a "Champion of Change" ... deployment in the residential, commercial and industrial sectors. ... Electrical and Computer Engineering and director of Clemson,s ... hero leading the charge across the country to ... and driving policy changes at the local level ...
(Date:4/17/2014)... a novel method to help kidney stone sufferers ... treatment possible., Kidney stones represent a major medical ... left untreated, apart from being particularly painful, they ... In many patients treated successfully, stone recurrence is ... approach to diagnosis and treatment needs to be ...
Breaking Biology News(10 mins):How vision makes sure that little fish do not get carried away 2How vision makes sure that little fish do not get carried away 3How vision makes sure that little fish do not get carried away 4White House honors Clemson professor as 'Champion of Change' for solar deployment 2
... shedding light on possible causes of infertility and autoimmune ... and florescent genes to illuminate cells that produce a ... lupus. The technique will help scientists track the ... ensuring supplies of breast milk in nursing mothers but ...
... Belfast academic has shown that crabs not only suffer pain ... study, which looked at the reactions of hermit crabs to ... and Mirjam Appel from the School of Biological Sciences at ... Behaviour . Professor Elwood, who previously carried out a ...
... fish stocking has on aquatic insects in mountain lakes ... to a study completed by U.S. Forest Service and ... a current online issue of the journal Freshwater Biology ... fisheries management practices now in use in California mountain ...
Cached Biology News:Crabs' memory of pain confirmed by Queen's academic 2Study assesses impact of fish stocking on aquatic insects 2
(Date:1/15/2014)... DTS Language Services, Inc . is pleased to ... Science organizations who need document translations. Clients will now ... documents in advance with a selection of nearly 50 life ... a critical factor in clinical and scientific fields, and decrease ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. ... Road, Warrington, Pa. , Details: The Hepatitis B ... a cure for hepatitis B and improving the quality of ... Ball on Friday, April 11 at Warrington Country Club in ...
(Date:1/14/2014)... 14, 2014 EquitiesIQ, a leading informational ... ALQA). Alliqua is an emerging biomedical company acquiring, developing, ... care market. , Free report download: http://equitiesiq.com/reports/alliqua/ ... seasoned management team and Board, which launched the company’s ...
(Date:1/14/2014)... , Jan. 14, 2014  RXi Pharmaceuticals Corporation ... developing and commercializing innovative therapies addressing major unmet ... it received the Notice of Allowance from the ... unique self-delivering RNAi compounds (sd-rxRNA®), for the treatment ...
Breaking Biology Technology:DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3
... Globally, approximately 418 million,people are currently infected with ... hepatitis in the 21st century cannot be,understated or ... by Kalorama Information. Paradoxically, despite the obvious ... to contain hepatitis. Yet the incidence and,prevalence of ...
... Tuesday, September 11, 2007 - 11:00 a.m. ET; 8:00 ... 6 /PRNewswire-FirstCall/ - MIGENIX,Inc. (TSX: MGI; OTC: MGIFF), a ... conference call and webcast on Tuesday,September 11, 2007 at ... for the first fiscal quarter ended July 31, 2007. ...
... Inc.,(Nasdaq: VRUS ) and Roche (OTC: RHHBY) ... for the treatment of chronic hepatitis C,virus (HCV) ... on Hepatitis C,Virus and Related Viruses being held ... prodrug of PSI-6130, is an oral cytidine nucleoside,analog ...
Cached Biology Technology:Touching 418 Million Lives, Hepatitis Still Presents Significant Threat to Global Health 2MIGENIX First Fiscal Quarter 2008 Financial Results Conference Call & Webcast Notification 2Pharmasset and Roche to Present R7128 Data at the 14th International Symposium on Hepatitis C Virus and Related Viruses 2Pharmasset and Roche to Present R7128 Data at the 14th International Symposium on Hepatitis C Virus and Related Viruses 3Pharmasset and Roche to Present R7128 Data at the 14th International Symposium on Hepatitis C Virus and Related Viruses 4Pharmasset and Roche to Present R7128 Data at the 14th International Symposium on Hepatitis C Virus and Related Viruses 5
Mouse monoclonal antibody raised against a partial recombinant ARHGEF11. NCBI Entrez Gene ID = ARHGEF11...
Mouse monoclonal antibody raised against a partial recombinant MYO7A. NCBI Entrez Gene ID = MYO7A...
... PrecisION Ion Channel Cell Lines ,High ... ,Ion channels are well known for having ... function and consequently have a key function ... that modulate ion channels have been investigated ...
... Channel Cell Lines ,High Quality, Functionally-Validated, Ion ... well known for having a critical role ... have a key function in pain, CNS ... channels have been investigated in therapeutic areas, ...
Biology Products: