Navigation Links
New RNAi tools enable systematic studies of gene function

s tailored to match a fragment of a gene's unique DNA. This RNA can then bind to its gene target, rendering it inactive. In order to get the small RNAs into cells, TRC scientists packaged them in lentiviruses. "Across the spectrum of biomedicine, there is a need for tools that can be applied to diverse cell types. This is particularly true in cancer research," said Bill Hahn, assistant professor at Dana-Farber Cancer Institute and Harvard Medical School, associate member of the Broad Institute and a senior author of the study. "For TRC's library, lentiviral delivery is an especially effective means to meet this need."

The parallel analysis of thousands of genes using RNAi allows researchers to more readily pinpoint the genes that control a biological process. Therefore, TRC developed the high-throughput techniques and quality-control measures required for such genome-scale studies. "It is a distinct challenge to achieve consistent and cost-effective RNAi methods and we placed a strong emphasis on this part of the process," said David Sabatini, member of Whitehead Institute for Biomedical Research, assistant professor at Massachusetts Institute of Technology, associate member of the Broad Institute and a senior author. "In the quest to develop comprehensive tools for gene discovery in mice and humans, this technology will be a key piece in the puzzle."

To evaluate the RNAi library's performance, the scientists sampled a subset that targets approximately 1,000 human genes. They systematically inactivated these genes in a human cancer cell line to identify ones that regulate cell division during malignancy. Automated cellular imaging was used to efficiently identify dividing cells in thousands of samples. This approach uncovered more than 100 previously unknown growth regulators in addition to several known players, confirming the library's sensitivity as a vehicle for gene discovery.

"This critical new tool illustrates the requirement for acad
'"/>

Source:Broad Institute of MIT and Harvard


Page: 1 2 3

Related biology news :

1. Improved statistical tools reveal many linked loci
2. Virginia Tech group adds tools to DNA-targeted anti-cancer drugs
3. New tools used to control foodborne hepatitis A outbreaks related to green onions
4. MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools
5. Nanoparticles, nanoshells, nanotubes: How tiny specks may provide powerful tools against cancer
6. Discovery of new molecular tools for biosynthesis could lead to advances in use of pectin
7. Recycled paper and compost could both be key tools to control plant disease
8. Nanotech tools yield DNA transcription breakthrough
9. Molecular tools make the cut
10. Chimpanzees found to use tools to hunt mammalian prey
11. Recombinant DNA technology may enable oral, rather than injectable, delivery of protein drugs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New RNAi tools enable systematic studies gene function

(Date:4/18/2014)... NC Seeds that sprout as soon as they,re planted ... need to be more careful. In the wild, a plant ... day would risk disaster. More than just an insurance policy ... that seed dormancy has long-term advantages too: Plants whose ... rise to more species, finds in a team of researchers ...
(Date:4/17/2014)... , Respiratory syncytial virus (RSV) is a ... life-threating illness in very young and elderly populations. ... enters host cells through the fusion protein RSV ... interfering peptides that prevent bundle formation limit RSV ... susceptible to degradation. In this issue of the ...
(Date:4/17/2014)... Gauthier and Professor Luca Razzari of the nergie ... large grants from the John R. Evans Leaders ... for the acquisition of state-of-the-art biotech and nanophotonics ... grants from the Ministre de l,Enseignement suprieur, de ... Technologie (MESRST). These new laboratories will help us ...
Breaking Biology News(10 mins):Plants with dormant seeds give rise to more species 2JCI online ahead of print table of contents for April 17, 2014 2JCI online ahead of print table of contents for April 17, 2014 3JCI online ahead of print table of contents for April 17, 2014 4JCI online ahead of print table of contents for April 17, 2014 5JCI online ahead of print table of contents for April 17, 2014 6New state-of-the-art biotech and nanotech equipment for INRS 2
... bio-based plastic packaging is not only eco-friendly but also ... The plastic packaging industry is moving towards completely bio-based ... the production of plastics equates to approximately five per ... per cent of all plastics are used in packaging, ...
... well-known fairy tale, Rumpelstiltskin used magic to weave straw into ... to turn straw (and other forms of biomass) into today,s ... in which minute particles of gold dot the surface of ... two materials are strong enough to breaks the O-O bond ...
... Software Inc. of Ottawa, Canada announced today it ... lead its company awareness and lead generation campaigns. ... in developing integrated marketing programs that target embedded ... messaging and positioning, PR, content development, partner co-marketing ...
Cached Biology News:Plastic packaging industry is moving towards completely bio-based products 2Gases from grasses 2Gases from grasses 3Crank Software Selects TREW Marketing for Embedded Industry Outreach 2Crank Software Selects TREW Marketing for Embedded Industry Outreach 3
(Date:1/15/2014)... The Microcompetition with Foreign DNA theory explains how latent ... viruses is the Epstein Barr Virus (EBV), and one of ... a chronic inflammatory disease that destroys the body’s joints. As ... have high concentrations of EBV DNA in their bodies (1). ...
(Date:1/15/2014)... AudioNotch is the internet's leading provider of ... of tinnitus. Patients listen to sound therapy that is ... of weeks to months, their tinnitus volume decreases. , AudioNotch ... Music and Notched White Noise. Now, AudioNotch is pleased to ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company developing ... simulated embryonic conditions, today announced that they have entered ... for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent cell ... an amendment to the existing license between Histogen and ...
(Date:1/14/2014)... Texas , Jan. 14, 2014 The ... and research medicinal plants and therapeutic derivatives thereof ... health professionals, and researchers about the challenges of ... http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) The Society ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8
... Aug. 14 Pressure,BioSciences, Inc. (Nasdaq: PBIO ) ... the second quarter of 2008 was $120,184 compared to,$202,127 ... from the sale,of PCT products and services was $117,698 ... to $136,355 for the prior year period. During the,second ...
... drugs and devices that have ... the quality of human life -, PALO ALTO, Calif., ... announced that its chronic angina therapy,Ranexa(R) (ranolazine extended-releases tablets) has ... 2008 award which honors the,best pharmaceutical agent and best biotechnology ...
... TROY, Mich., Aug. 14 CJPS Enterprises, LLC, ... today that it,has put online more resources for ... market, or to grow their existing healthcare,business. Those ... This project is dedicated to providing users ...
Cached Biology Technology:Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 2Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 3Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 4Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 5Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 6Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 7Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 8Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update 9CV Therapeutics Announces Ranexa(R) Named Finalist for Prix Galien USA 2008 Award 2CV Therapeutics Announces Ranexa(R) Named Finalist for Prix Galien USA 2008 Award 3The Michigan Life Sciences Pipeline Adds More Resources On Its Website 2
...
The BD CBA Human Apoptosis Kit can be used to quantitatively measure,cleaved PARP, Bcl-2, and active Caspase-3 protein levels in a single sample. The,Kit performance has been optimized for analysis o...
... package that provides crystallographers with an ... creation, set-up of experimental conditions and ... software helps crystallographers through the most ... Design of Crystallization ...
BD Falcon insert HTS 96 well, square A-bottom with lid. PET membrane, sterile. (5/ca) Packaging: 5 / pack, 5 / case ...
Biology Products: