Navigation Links
Neuronal cell cultures kept on the straight and narrow

An improved technique for culturing cells, developed at the National Institute of Standards and Technology (NIST), may enable new, fundamental insights into the behavior of neuronal cells.

Culturing particular types of cells in isolation is a basic technique for measuring how they respond to various stimuli, testing new drugs, and similar cell biology tasks. Neuronal cells, which make up the central nervous system in mammals, are both particularly important and particularly hard to culture. They are highly specialized and choosy about their environment--normally they only survive and develop when cultured on a layer of non-neuronal "glial" cells that provide cellular support services. There are usually far more glial cells than neuronal cells, which makes it hard to image neuronal cells and measure their activity against the glial background.

In a paper in the American Chemical Society's journal Langmuir*, NIST researchers detail a microfluidics technique to culture neuronal cells in relative isolation on a variety of cell-culture surfaces, and to pattern the cells on the surface to study the effects of geometry on cell development. The trick is to mask the substrate with multiple alternating layers of positively and negatively charged polymers, building up a so-called polyelectrolyte multilayer (PEM). Properly selected, the PEM coating convinces the neuronal cells that they're in a good environment to attach, develop and produce the characteristic neuron projections and synapses, all without a glial layer.

Even better, according to the NIST team, microfluidic channels can be used to lay down the PEM coating in patterned lines just a few micrometers wide. Neuronal cells will largely confine themselves to the pattern, enabling a variety of cell-geometry experiments, such as measuring the maximum gap between lines that can be bridged by neural axons and dendrites.

The research is part of a multidisciplinary NIST program to develop bioche mical measurement technologies based on microfluidics.


'"/>

Source:National Institute of Standards and Technology (NIST)


Related biology news :

1. Neuronal traffic jam marks early Alzheimers disease
2. Molecular Partners Required For Appropriate Neuronal Gene Repression
3. Study IDs protein that inhibits HIV from growing in cell cultures
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/14/2017)... , June 15, 2017  IBM (NYSE: IBM ) ... international tech event dedicated to developing collaboration between startups and ... on June 15-17. During the event, nine startups will showcase ... value in various industries. France ... the international market, with a 30 percent increase in the ...
(Date:5/16/2017)...   Bridge Patient Portal , an enterprise ... EMR Systems , an electronic medical record solutions ... established a partnership to build an interface between ... Centricity™ products, including Centricity Practice Solution (CPS), Centricity ... new integrations will allow healthcare delivery networks using ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:9/19/2017)... ... ... Biopharma ’s CEO and founder, Dr. Bob Harman DVM, MPVM, is featured in an interview ... Rising Tide". Dr. Harman and Dr. Riordan met in 2003 and have remained in ... of stem cell therapy and a fast friendship was formed. , Dr. Harman has been ...
(Date:9/19/2017)... , Sept. 19, 2017 ValGenesis Inc., ... (VLMS) is pleased to announce the strategic partnership with ... provide clients with validation services using the latest technology ... VTI will provide clients with efficient and cost-effective validation ... marketing partner for the ValGenesis VLMS system. ...
(Date:9/19/2017)... ... September 19, 2017 , ... ... Financial Services (NYS DFS) cybersecurity regulations have transitioned into full force ... organizations operating in the state (“Covered Entities”) to conduct an annual, professional, ...
(Date:9/18/2017)... ... September 18, 2017 , ... Scholarly publisher ... the SPIE Digital Library ( http://www.spiedigitallibrary.org ) on 15 August to a new ... user experience and incorporate a number of enhancements and new features, the website ...
Breaking Biology Technology: