Navigation Links
Neuronal cell cultures kept on the straight and narrow

An improved technique for culturing cells, developed at the National Institute of Standards and Technology (NIST), may enable new, fundamental insights into the behavior of neuronal cells.

Culturing particular types of cells in isolation is a basic technique for measuring how they respond to various stimuli, testing new drugs, and similar cell biology tasks. Neuronal cells, which make up the central nervous system in mammals, are both particularly important and particularly hard to culture. They are highly specialized and choosy about their environment--normally they only survive and develop when cultured on a layer of non-neuronal "glial" cells that provide cellular support services. There are usually far more glial cells than neuronal cells, which makes it hard to image neuronal cells and measure their activity against the glial background.

In a paper in the American Chemical Society's journal Langmuir*, NIST researchers detail a microfluidics technique to culture neuronal cells in relative isolation on a variety of cell-culture surfaces, and to pattern the cells on the surface to study the effects of geometry on cell development. The trick is to mask the substrate with multiple alternating layers of positively and negatively charged polymers, building up a so-called polyelectrolyte multilayer (PEM). Properly selected, the PEM coating convinces the neuronal cells that they're in a good environment to attach, develop and produce the characteristic neuron projections and synapses, all without a glial layer.

Even better, according to the NIST team, microfluidic channels can be used to lay down the PEM coating in patterned lines just a few micrometers wide. Neuronal cells will largely confine themselves to the pattern, enabling a variety of cell-geometry experiments, such as measuring the maximum gap between lines that can be bridged by neural axons and dendrites.

The research is part of a multidisciplinary NIST program to develop bioche mical measurement technologies based on microfluidics.


'"/>

Source:National Institute of Standards and Technology (NIST)


Related biology news :

1. Neuronal traffic jam marks early Alzheimers disease
2. Molecular Partners Required For Appropriate Neuronal Gene Repression
3. Study IDs protein that inhibits HIV from growing in cell cultures
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a provider ... MegaMatcher Automated Biometric Identification System (ABIS) , ... multi-biometric projects. MegaMatcher ABIS can process multiple complex ... any combination of fingerprint, face or iris biometrics. ... SDK and MegaMatcher Accelerator , which ...
(Date:4/19/2016)... , UAE, April 20, 2016 ... be implemented as a compact web-based "all-in-one" system solution ... the biometric fingerprint reader or the door interface with ... of modern access control systems. The minimal dimensions of ... ID readers into the building installations offer considerable freedom ...
(Date:3/31/2016)... March 31, 2016   ... or the "Company") LegacyXChange is excited to ... its soon to be launched online site for trading ... ) will also provide potential shareholders a sense ... technology to an industry that is notorious for fraud. ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, ... faculty of the University of North Carolina Kenan-Flagler Business School effective ... at UNC Kenan-Flagler, with a focus on the school’s international efforts, leading classes ...
(Date:6/27/2016)... 27, 2016   Ginkgo Bioworks , a leading ... was today awarded as one of the World ... world,s most innovative companies. Ginkgo Bioworks is engineering ... real world in the nutrition, health and consumer ... with customers including Fortune 500 companies to design ...
(Date:6/24/2016)... , June 24, 2016 Epic ... sensitively detects cancers susceptible to PARP inhibitors by ... tumor cells (CTCs). The new test has already ... therapeutics in multiple cancer types. Over ... DNA damage response pathways, including PARP, ATM, ATR, ...
(Date:6/24/2016)... ... , ... Researchers at the Universita Politecnica delle Marche in Ancona combed medical ... mesothelioma. Their findings are the subject of a new article on the Surviving Mesothelioma ... in the blood, lung fluid or tissue of mesothelioma patients that can help point ...
Breaking Biology Technology: