"Up until now motors and brakes were studied separately from microtubules," says senior author Phong Tran, PhD, Assistant Professor of Cell and Developmental Biology. "This study lets us have a more complete picture."
Microtubules are structures that help give shape to many types of cells, form the spindle -- a structure important in cell division -- and act as a railroad, of sorts, upon which molecular motors move protein packages for waste removal and nerve transmission.
In the Cell study, the investigators, working with fission yeast cells, showed that stable end-to-end arrays of microtubules can be achieved by a balance between the sliding by a molecular motor called klp2p and the braking of a microtubule-associated protein (MAP) called ase1p. Specifically, they showed that a preexisting "mother" microtubule acts as a platform on which a new microtubule can be formed. The new "daughter" microtubule grows and moves along the mother microtubule. In time, the daughter grows beyond the end of the mother to ultimately produce two microtubules, connected by the cross-linking MAP ase1p.
"Imagine that the daughter microtubule is a short train on the track of the mother microtubule," explains Tran. "The molecular motor is the train's engine, but the problem is that the cargo -- the molecular brakes -- gets longer, slowing down the daughter train. But when the train gets to the end of the trac
Source:University of Pennsylvania School of Medicine