Navigation Links
Microreactor efficiently regenerates cofactors for biocatalysis

One of the longstanding challenges in the synthesis of pharmaceuticals, cosmetics and food additives is the continuous regeneration of molecules called cofactors that permit the synthesis through inexpensive and environmentally friendly biocatalytic processes.

Now, a team of researchers from the University of Illinois at Urbana-Champaign and the Universite Paul Sabatier in Toulouse, France, has developed a microreactor that efficiently regenerates cofactors through enzyme-catalyzed reactions.

"Enzymes are nature's catalysts, but in some cases, enzymes can not prompt a speedy chemical reaction," said Paul Kenis, a professor of chemical and biomolecular engineering at Illinois and a researcher at the Beckman Institute for Advanced Science and Technology. "In those cases, one or more cofactors are required."

By continuously regenerating the required cofactors, the microreactor enables the desired biocatalytic processes. Kenis and his colleagues describe their work in a paper that has been accepted for publication in the Journal of the American Chemical Society, and posted on its Web site.

The microreactor uses a Y-shaped microfluidic channel in which two liquid streams (a reactant stream and a buffer stream) merge and flow laminarly between two electrodes without mixing. By adjusting the flow rates of the two streams, the researchers can focus the reactant stream close to the cathode, and a normally unfavorable reaction equilibrium is driven into the desired direction of cofactor regeneration.

"In large batch reactors, a spontaneous reverse reaction prevents the regeneration of essential cofactors," Kenis said. "The absence of a bulk phase in our microreactor prevents the unwanted reverse reaction from occurring, while permitting continuous operation."

Using their microreactor, the researchers performed a model biocatalytic process by converting an achiral substrate (pyruvate) into a chiral product (L-lactate), using lacta te dehydrogenase as the enzyme.

While further research is needed to improve the performance of individual microreactors, the present work shifts the emphasis from the longstanding problem of cofactor regeneration to a more tangible engineering challenge, Kenis said. "Now we need to integrate a large number of these microreactors in a recirculating system to enable the biocatalytic synthesis of chiral fine chemicals in larger quantities."


'"/>

Source:University of Illinois at Urbana-Champaign


Related biology news :

1. Brain regions do not communicate efficiently in adults with autism
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/20/2016)... , May 20, 2016  VoiceIt is ... partnership with VoicePass. By working together, ... experience.  Because VoiceIt and VoicePass take slightly different ... engines increases both security and usability. ... excitement about this new partnership. "This ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
(Date:4/26/2016)... DUBLIN , April 27, 2016 ... of the  "Global Multi-modal Biometrics Market 2016-2020"  report ... ) , The analysts forecast ... a CAGR of 15.49% during the period 2016-2020.  ... a number of sectors such as the healthcare, ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, one of the ... brand, UP4™ Probiotics, into Target stores nationwide. The company, which has been manufacturing ... to its list of well-respected retailers. This list includes such fine stores as ...
(Date:6/23/2016)... - FACIT has announced the creation of a ... Propellon Therapeutics Inc. ("Propellon" or "the Company"), to ... of first-in-class WDR5 inhibitors for the treatment of ... an exciting class of therapies, possessing the potential ... patients. Substantial advances have been achieved with the ...
(Date:6/23/2016)... 23, 2016 Houston Methodist Willowbrook Hospital ... Sports Association to serve as their official health ... Methodist Willowbrook will provide sponsorship support, athletic training ... association coaches, volunteers, athletes and families. ... Sports Association and to bring Houston Methodist quality ...
(Date:6/23/2016)... 2016  The Prostate Cancer Foundation (PCF) is pleased to announce ... cures for prostate cancer. Members of the Class of 2016 were selected from ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
Breaking Biology Technology: