Navigation Links
Microreactor efficiently regenerates cofactors for biocatalysis

One of the longstanding challenges in the synthesis of pharmaceuticals, cosmetics and food additives is the continuous regeneration of molecules called cofactors that permit the synthesis through inexpensive and environmentally friendly biocatalytic processes.

Now, a team of researchers from the University of Illinois at Urbana-Champaign and the Universite Paul Sabatier in Toulouse, France, has developed a microreactor that efficiently regenerates cofactors through enzyme-catalyzed reactions.

"Enzymes are nature's catalysts, but in some cases, enzymes can not prompt a speedy chemical reaction," said Paul Kenis, a professor of chemical and biomolecular engineering at Illinois and a researcher at the Beckman Institute for Advanced Science and Technology. "In those cases, one or more cofactors are required."

By continuously regenerating the required cofactors, the microreactor enables the desired biocatalytic processes. Kenis and his colleagues describe their work in a paper that has been accepted for publication in the Journal of the American Chemical Society, and posted on its Web site.

The microreactor uses a Y-shaped microfluidic channel in which two liquid streams (a reactant stream and a buffer stream) merge and flow laminarly between two electrodes without mixing. By adjusting the flow rates of the two streams, the researchers can focus the reactant stream close to the cathode, and a normally unfavorable reaction equilibrium is driven into the desired direction of cofactor regeneration.

"In large batch reactors, a spontaneous reverse reaction prevents the regeneration of essential cofactors," Kenis said. "The absence of a bulk phase in our microreactor prevents the unwanted reverse reaction from occurring, while permitting continuous operation."

Using their microreactor, the researchers performed a model biocatalytic process by converting an achiral substrate (pyruvate) into a chiral product (L-lactate), using lacta te dehydrogenase as the enzyme.

While further research is needed to improve the performance of individual microreactors, the present work shifts the emphasis from the longstanding problem of cofactor regeneration to a more tangible engineering challenge, Kenis said. "Now we need to integrate a large number of these microreactors in a recirculating system to enable the biocatalytic synthesis of chiral fine chemicals in larger quantities."


'"/>

Source:University of Illinois at Urbana-Champaign


Related biology news :

1. Brain regions do not communicate efficiently in adults with autism
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/8/2016)... -- Singulex, Inc., the leader in Next Generation Immunodiagnostics powered ... and supply agreement with Thermo Fisher Scientific, the world ... to Thermo Scientific BRAHMS PCT (Procalcitonin), a biomarker which, ... diagnose systemic bacterial infection and sepsis and in ... assessing the risk of critically ill patients for progression ...
(Date:12/7/2016)... LONDON , Dec. 7, 2016   Avanade ... most successful Formula One teams in history, exploit biometric ... pit stop performance and maintain the competitive edge against ... in 2016. Avanade has worked with ... a range of biometric data (heart rate, breathing rate, ...
(Date:12/6/2016)... , Dec. 6, 2016 Valencell , ... today it has seen a third consecutive year of ... sensor technology in 2016 with a 360 percent increase ... year. This increase was driven by sales of its ... robust interest in its technology for hearables for fitness ...
Breaking Biology News(10 mins):
(Date:1/12/2017)... ... ... Each year, Crain’s Detroit Business News ranks the most innovative companies through ... of a company, its impact and significance, and the likelihood of bringing it to ... that transform energy sources such as low dose X-ray and convert them into energy ...
(Date:1/12/2017)... ... ... Huffman Engineering, Inc. , a leader in control systems integration, today announced ... as a chemical engineer. In his new role, Beck will use his extensive ... manufacturing and water/wastewater industries. , Prior to joining Huffman Engineering, Beck served for nine ...
(Date:1/12/2017)... -- The Energy and Resources Institute (TERI) ... mycorrhizae. The Centre for Mycorrhizal Research at TERI has ... developed a technology that eventually produces mycorrhizae based biofertilizer. ... ... TERI facility has a production capacity of over 600 ...
(Date:1/12/2017)... 12, 2017 A new report published by Allied Market Research ... Opportunity Analysis and Industry Forecast, 2014-2022," projects that the global in vitro toxicity testing ... a CAGR of 15.07% during the forecast period. Continue ... ... ...
Breaking Biology Technology: