Navigation Links
Making a face: A new and earlier marker of neural crest development

The fate of cells that go on to form the face, skull and nerve centers of the head and neck in vertebrates is determined much earlier in development than previously thought, and is independent of interaction with other forming tissues, according to a recent study published in the journal Nature.

The collaborators at Yale and Caltech demonstrate with three different technologies -- immunostaining of proteins, in situ hybridization and multiplex RT-PCR of mRNAs -- that formation of neural crest cells in chick embryos is independent of both mesoderm and neural tissues. They also identify, Pax7, as an early marker of neural crest formation and prove that its function is required in the earliest stages of development.

The neural crest is a population of stem cells that migrate extensively during development and give rise to many derivatives, including most of the bone and cartilage of the head skeleton, pigment cells of the skin, and cells of the peripheral nervous system.

In humans, cleft palate, heart valve malformations and various tumors are among the common malformations associated with disruption of neural crest development.

Chick embryos have well-characterized stages and are a valuable model for examining vertebrate development. While it was known that the ability to form neural crest cells declines after "stage 10," the researchers were seeking the earliest conditions surrounding formation of these important stem cells.

"Understanding the origin of neural crest cells -- where, when and how they arise -- is a critical step if we are to manipulate them for therapeutic purposes," said Martín García-Castro, assistant professor of molecular, cellular and developmental biology at Yale and principal investigator on the study. "Implications of these basic questions of biology and development reach far beyond these chicken and eggs."

Based on w ork from the 1940's before molecular tools were available, the neural crest was thought to form by interactions between neural and non-neural cell layers. "We show in this work that neural crest stem cell precursors are designated very early in development -- as early as the gastrula stages -- and in an independent fashion from those other tissues," said Martín García-Castro.

The researchers grew grafts of cells from "stage 3" chick embryos, before the neural plate formed, in non-inducing cultures. Surprisingly, restricted regions of the embryo generated both migrating neural crest cells and their derivative cell types, without any interaction with neural or mesodermal tissues.

"Our results are contrary to current text-book models and suggest that different modes of neural crest induction operate during development," said Martín García-Castro. "Interestingly, the one we have uncovered is related to the early, cranial neural crest cells, the only ones in higher vertebrates that retain bone and cartilage forming potential."


'"/>

Source:Yale University


Related biology news :

1. Making Sure Sacred Sheep Dont Become Extinct
2. Muscle repair: Making a good system better, faster; implications for aging, disease
3. Making plant cells work like miniature factories
4. Making medicine smarter
5. Making mice with enhanced color vision
6. Asymptomatic HIV babies could use earlier treatment
7. With cochlear implants, earlier use leads to better speech
8. Biomarkers isolated from saliva successfully predict oral and breast cancer
9. UCLA cancer researchers shake loose hidden biomarker
10. Measurement Challenges In Detecting Cancer Biomarkers
11. New markers of climate change
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/26/2017)... , Jan. 26, 2017  Acuity Market Intelligence ... Biometrics and Digital Identity".  Acuity characterizes 2017 as ... when increased adoption reflects a new understanding of ... "Biometrics and digital identity are often perceived ... Maxine Most , Principal of Acuity Market intelligence. ...
(Date:1/23/2017)... -- The latest mobile market research from Acuity Market Intelligence ... quarterly average price of a biometric smartphone decreased from ... There are now 120 sub-$150 models on the market ... 28 a year ago at an average price of ... , Acuity Market Intelligence Principal, "Biometric Smartphones are a ...
(Date:1/18/2017)... 18, 2017  In vitro diagnostic (IVD) companies were ... acquisitions (M&A), and Kalorama Information expects that trend to ... been shifting. Generally, uncertainty in reimbursement and healthcare reform ... has changed the acquisitions landscape. Instead of looking to ... buying partners outside of their home country and also ...
Breaking Biology News(10 mins):
(Date:2/22/2017)... , ... February 22, 2017 , ... ... Dr. Daniel Spyker, PhD, MD former Acting Deputy Director in the FDA CDRH ... Drug Evaluation Staff has joined the company as an Expert Consultant. , In ...
(Date:2/22/2017)... and RESEARCH TRIANGLE PARK, N.C., Feb. 22, 2017 /PRNewswire/ ... today announced its financial results for the fourth ... "Our annual 2016 financial results reflect continued growth ... exceeded $700 million," said Martine Rothblatt, Ph.D., United ... results strengthen our ability to develop and advance ...
(Date:2/21/2017)... ... February 21, 2017 , ... During HIMSS ... digital health applications, announced a partnership with Redox, a leader in cloud-based healthcare ... many clinical systems while keeping data secure in the cloud. , The digital ...
(Date:2/21/2017)... ... February 21, 2017 , ... Cancer diagnostics ... has joined its executive team to lead the development and commercialization of its ... help improve the diagnosis and treatment of cancer. The CIDT addresses the need ...
Breaking Biology Technology: