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Loss of a universal tRNA feature reported

as all other bacteria do. Amplification and tRNA sequencing approaches were used to confirm the findings.

Sinorhizobium meliloti and Caulobacter crescentus are members of the alphaproteobacteria, a group of diverse organisms whose members have successfully adopted different lifestyle and energy-yielding strategies in the course of evolution. The organisms are members of a specific group of alphaproteobacteria that comprises the Rhizobiales, Rhodobacterales, Caulobacterales, Parvularculales and Pelagibacter.

Dieter Söll, Sterling Professor of Molecular Biophysics and Biochemistry in the Department of Molecular Biophysics and Biochemistry at Yale University, commented: "The observed absence of the extra guanylate in tRNAHis is a unique case of divergence from a highly conserved ancient tRNA recognition mechanism by an aminoacyl-tRNA synthetase. It will be of special interest to examine if the loss of this tRNA feature has resulted from the presence of an RNase P enzyme that is no longer capable of catalyzing abnormal cleavage at the -1 position in the tRNAHis precursor. It will also be revealing to see how different aminoacyl-tRNA synthetases have adapted to discriminate between their cognate or related substrates and the now less distinctive tRNAHis."


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Source:Virginia Tech


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