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Live Recombinant Adenovirus Vaccine Technique Explored

HPV major capsid protein (L1) provides effective immunity to HPV infection. Dr. Ketner and his colleagues theorized that a live recombinant adenovirus could be made to express L1 as the virus replicated in the body and thereby create a lasting immune response. The researchers inserted the L1 gene into a live adenovirus such that it would be expressed using the virus' own late-gene expression machinery. L1 is expressed at very high levels in some recombinants.

Dr. Ketner believes that the replicating live recombinant adenovirus model shows promise as a means of developing economical and long-lasting vaccines for many illnesses. Ongoing research is aimed at determining the effectiveness of recombinants in animals.

Additional authors of “Viable adenovirus vaccine prototypes: High-level production of a papillomavirus capsid antigen from the major late transcriptional unit?include Michael Berg, Julie DiFatta and Egbert Hoiczyk of the W. Harry Feinstone Department of Molecular Microbiology and Immunology at the Johns Hopkins Bloomberg School of Public Health. Author Richard Schlegel is with the Department of Pathology at the Georgetown University Medical Center.

Funding was provided by grants from the National Institutes of Health.


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Source:Johns Hopkins Bloomberg School Of Public Health


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