Navigation Links
Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants

Just over a decade ago, biologists isolated a unique protein from jellyfish that could be inserted into other organisms—from E. coli to pigs—and cause them to radiate a brilliant green color. This green fluorescent protein (GFP) has allowed biologists to make many new discoveries regarding how living cells function. But one kingdom of life—plants—has presented special challenges to GFP detection: plants harbor tough cell walls and enormous subcellular structures that interfere with visualization, and their natural green pigments can mask the luminescent qualities of GFP.

The current issue of Cold Spring Harbor Protocols includes a freely available article that addresses these concerns (http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31). It provides advice on choosing appropriate plant tissues, designing test proteins for maximal GFP detection, and setting up microscope equipment for imaging in plants. This information will be useful to a broad range of scientists interested in plant biology and imaging technologies.

A second freely accessible article (http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.prot4674)—also new to Cold Spring Harbor Protocols this month—describes a procedure for nurturing mammalian cells for studies in cell division. Both of these publications join a growing library of high-quality methods from Cold Spring Harbor Protocols that span a broad spectrum of topics essential to researchers across many disciplines.


'"/>

Source:Cold Spring Harbor Laboratory


Page: 1

Related biology news :

1. A bugs life: Exceptional genomic stability yet rapid protein evolution in a carpenter ant mutualist
2. The shape of life: research sheds light on how cells take shape
3. Selecting life: Scientists find new way to search for origin of life
4. Spring migration of pink-footed geese under threat
5. Owl genomics presents a HEPATOCHIP for diagnosis of non-alcoholic steatohepatitis
6. MIT researcher presents new view of how the cortex forms
7. The quest for an effective HIV vaccine presents new possibilities, challenges
8. Researchers unlock mechanism creating jigsaw puzzle-like plant cells
9. By creating molecular bridge, scientists change function of a protein
10. Researchers devise new technique for creating human stem cells
11. Lands surface change on Alaska tundra creating longer, warmer summers in Arctic
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/13/2016)...  IMPOWER physicians supporting Medicaid patients in ... standard in telehealth thanks to a new partnership with ... IMPOWER patients can routinely track key health measurements, such ... and, when they opt in, share them with IMPOWER ... local retail location at no cost. By leveraging this ...
(Date:3/23/2016)... Massachusetts , March 23, 2016 /PRNewswire/ ... im Interesse erhöhter Sicherheit Gesichts- und Stimmerkennung ... Xura, Inc. (NASDAQ: MESG ), ... bekannt, dass das Unternehmen mit SpeechPro zusammenarbeitet, ... aus der Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, ...
(Date:3/18/2016)... --> --> Competitive Landscape Analysis ... Physical infrastructure and Perimeter Surveillance & Detection Systems ... and the continuing migration crisis in the Middle ... led visiongain to publish this unique report, which is crucial ... & security companies in the border security market and the ...
Breaking Biology News(10 mins):
(Date:5/23/2016)... OXFORD, England , May 23, 2016 ... Wednesday, May 25 th at 10:15 a.m. ET before ... about the role genetically engineered mosquitos can play in controlling ... known carrier of the Zika virus.      (Logo: ... genetically engineered male mosquito with a self-limiting gene. Trials in ...
(Date:5/23/2016)... Palo Alto, CA (PRWEB) , ... May 23, ... ... tank and public interest organization focused on molecular nanotechnology, announced the winners for ... honor of pioneer physicist Richard Feynman, are given in two categories, one for ...
(Date:5/20/2016)... ... 2016 , ... Kablooe Design, a leading provider of product design and development ... of the business. “We have worked hard to build long-term relationships,” says President and ... and honor of serving their product design and development needs through the years.” , ...
(Date:5/19/2016)... ... May 19, 2016 , ... Anton Paar USA, located in Ashland, Virginia is ... complete. The new structure adds a third office building to the current facilities. ... 2.4 acres of land, along with office space adjacent to the previous main building. ...
Breaking Biology Technology: