Navigation Links
Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants

Just over a decade ago, biologists isolated a unique protein from jellyfish that could be inserted into other organisms—from E. coli to pigs—and cause them to radiate a brilliant green color. This green fluorescent protein (GFP) has allowed biologists to make many new discoveries regarding how living cells function. But one kingdom of life—plants—has presented special challenges to GFP detection: plants harbor tough cell walls and enormous subcellular structures that interfere with visualization, and their natural green pigments can mask the luminescent qualities of GFP.

The current issue of Cold Spring Harbor Protocols includes a freely available article that addresses these concerns (http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31). It provides advice on choosing appropriate plant tissues, designing test proteins for maximal GFP detection, and setting up microscope equipment for imaging in plants. This information will be useful to a broad range of scientists interested in plant biology and imaging technologies.

A second freely accessible article (http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.prot4674)—also new to Cold Spring Harbor Protocols this month—describes a procedure for nurturing mammalian cells for studies in cell division. Both of these publications join a growing library of high-quality methods from Cold Spring Harbor Protocols that span a broad spectrum of topics essential to researchers across many disciplines.


'"/>

Source:Cold Spring Harbor Laboratory


Page: 1

Related biology news :

1. A bugs life: Exceptional genomic stability yet rapid protein evolution in a carpenter ant mutualist
2. The shape of life: research sheds light on how cells take shape
3. Selecting life: Scientists find new way to search for origin of life
4. Spring migration of pink-footed geese under threat
5. Owl genomics presents a HEPATOCHIP for diagnosis of non-alcoholic steatohepatitis
6. MIT researcher presents new view of how the cortex forms
7. The quest for an effective HIV vaccine presents new possibilities, challenges
8. Researchers unlock mechanism creating jigsaw puzzle-like plant cells
9. By creating molecular bridge, scientists change function of a protein
10. Researchers devise new technique for creating human stem cells
11. Lands surface change on Alaska tundra creating longer, warmer summers in Arctic
Post Your Comments:
(Date:4/24/2014)... largest study of animal intelligence to-date finds that animals with ... this week in the journal Proceedings of the National ... long history of research aimed at understanding the animal mind. ... make and use tools, read social cues, or even understand ... studies of animal intelligence have focused on only one or ...
(Date:4/24/2014)... The alligator snapping turtle is the largest river turtle ... pounds and living almost a century. Now researchers from ... it is not one species but three. , ... deep evolutionary divisions in this ancient reptile. , Once ... the main ingredient of Campbell,s Turtle Soup in the ...
(Date:4/23/2014)... have added "playwright" to their resumes in an effort ... onslaught of ethical issues as genomic science transitions to ... first sequenced. , Until now the plays of ... performed for, and by, their peers and others in ... classrooms. With Oxford University Press, publication of the ...
Breaking Biology News(10 mins):Animals with bigger brains, broader diets have better self control 2Animals with bigger brains, broader diets have better self control 3Two new US turtle species described 2Two new US turtle species described 3Bioethicists use theatrical narratives to bridge the gap between society and science 2Bioethicists use theatrical narratives to bridge the gap between society and science 3
... Columbia study has found traces of the bacteria listeria in ... food microbiologist Kevin Allen tested a total of 40 ready-to-eat ... seven large chain stores and 10 small retailers in Metro ... fish jerky. The findings published in a ...
... could do more than just save people the trouble ... researchers have found that the "universal" vaccine could for ... of flu by limiting the influenza virus, ability to ... named for their effectiveness against several flu strains ...
... Institute (IVI), an international organization based in Seoul devoted ... for the developing world, reached a milestone in its ... shigellosis or bloody diarrhea), a deadly disease that affects ... the bacterial pathogen Shigella , is a major ...
Cached Biology News:Traces of listeria found in Vancouver ready-to-eat fish products: UBC study 2'Universal' vaccines could finally allow for wide-scale flu prevention 2'Universal' vaccines could finally allow for wide-scale flu prevention 3'Universal' vaccines could finally allow for wide-scale flu prevention 4'Universal' vaccines could finally allow for wide-scale flu prevention 5IVI granted 2 US patents on dysentery vaccine inventions 2
(Date:1/15/2014)... Automotive, a group of car dealerships headquartered in ... Center and offering free oil change coupons for participation in ... 18 at the Bill Jacobs Cadillac and Chevrolet location from ... )  The Bill Jacobs Auto annual blood ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Cynvenio, a ... management through the genomic analysis of tumor cells in ... Finder™, a web-based cancer decision support application powered by ... with Cynvenio’s ClearID™ genomic test, Therapy Finder will provide ...
(Date:1/15/2014)... , January 15, 2014 A study has ... on the Formula 1 track could help to tackle the ... Technologies (MAT), Stowhealth (a GP surgery based in Stowmarket) and ... provider Simplyhealth. Telemetry technology, which is inspired by ...
(Date:1/15/2014)... January 15, 2014 DTS Language Services, ... Online Web Portal for Life Science organizations who need document ... the subject matter of their documents in advance with a ... reduce time-to-delivery of translations, often a critical factor in clinical ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2
... tech arena: even when the economy is down, people keep ... ventures (whether self-funded, friends-and-family backed or fueled some other creative ... jobs. They create lots of jobs. In fact, small businesses ... shows no sign of slowing. , ,It can be appealing ...
... Wis. Having judged last years a success, ... it has opened the second annual Wisconsin Governor's ... plans. , ,Interested businesses will submit 250-word business proposals ... information technology, and business services. The proposals will be ...
... children, December is full of excitement and anticipation of what may ... as I screen the final onslaught of companies vying for a ... wait until February to unwrap the selected 70 or so companies ... along the way that just wont wait until the conference ...
Cached Biology Technology:How to duck the 3 most common entrepreneurial hiring traps 2How to duck the 3 most common entrepreneurial hiring traps 3How to duck the 3 most common entrepreneurial hiring traps 4Governors business-plan competition starts another round 2Governors business-plan competition starts another round 3An early Christmas gift 2