Navigation Links
Largest synthetic gene ever built offers insights into anti-malarial drug resistance

ion with investigators at the National Institutes of Health, described in 2000 that the PfCRT gene is primarily responsible for the vast majority of drug resistance that leads to human death from malaria. Earlier this year, they also helped describe how the gene PfNHE likely contributes, and this turns out not to be as vital as PfCRT. Now, based in part on this study, they know that PfMDR1 is dependent on mutated PfCRT, and probably doesn't act independently.

"The contribution of PfMDR1 to anti-malarial drug resistance is important, but much smaller than what many researchers thought it would be," said Roepe. "Before the discovery of PfCRT, many thought PfMDR1 would be the primary culprit."

Roepe, and his two other co-authors, Georgetown researchers Linda Amoah, PhD, and Jacqueline Lekostaj, an MD/PhD student at Lombardi Comprehensive Cancer Center, employed an unusual method to construct the PfMDR1 gene. They first obtained the PfMDR1 protein sequence from the NIH PubMed data base. They then "back translated" the protein sequence using a computer program to change the parasite gene sequence − which combines nucleotide base pairs in a way that is found in no other life form − into one that could be "read" by yeast. The yeast are then able to produce the PfMDR1 protein, in its proper sequence, from this synthetic gene. They previously used this same technique to create and express a synthetic version of the much smaller PfCRT gene. PfMDR1 is made up of about 4,000 base pairs and its protein is particularly large, Roepe said, making the recent work much more challenging.

Already, analysis of these three anti-malarial drug resistance genes is painting a picture of how the parasite escapes destruction by most of the agents now used against it, he said.,

When a malaria parasite is transmitted through a mosquito bite, the mosquito injects the parasite into skin or blood, which then travels to the liver within minutes, grows
'"/>

Source:Georgetown University Medical Center


Page: 1 2 3 4

Related biology news :

1. Largest computational biology simulation mimics life’s most essential nanomachine
2. Largest study of human interactome reveals a novel way
3. Largest genomic search finds genes that may contribute to autism
4. Inexpensive, mass-produced genes core of synthetic biology advances at UH
5. Researchers find promising cancer-fighting power of synthetic cell-signaling molecule
6. Tagging pathogens with synthetic DNA barcodes
7. Breakthrough in micro-device fabrication combines biology and synthetic chemistry
8. Heparin prepared synthetically could replace animal-derived drug
9. Williams College biologist explores photosynthetic apparatus
10. New synthetic self-assembling macromolecules mimic nature
11. Locusts built-in surface analysis ability directs them to fly overland
Post Your Comments:
(Date:7/25/2014)... Canadian wildfires have been raging this summer and some of ... U.S. In this image collected by the Moderate Resolution ... 2014 a swath of smoke has descended over the Great ... interesting is the fire image from July 23, 2014 (first ... smoke as it drifts off southeastward. In the image, ...
(Date:7/25/2014)... Birla, associate professor of biomedical engineering at the ... on tissue engineering and artificial organ development., "Introduction ... comprehensive guide to entering into the field of ... professor of biomedical engineering at UH, served as ... that while there are other published books on ...
(Date:7/25/2014)... Spina bifda (SB) is a complex congenital central ... incomplete closing of the neural tubes during the ... spasticity, urinary and fecal incontinence and neurocognitive retardation. ... their quality of life. Researchers at Ankara Physical ... investigate the functional performance in children with SB, ...
Breaking Biology News(10 mins):University of Houston researcher publishes textbook on tissue engineering 2
... scientific studies of the effects on wildlife of large-scale ... assess their impact, especially in the desert Southwest, according ... Geological Survey and published in the journal BioScience ... the paper, USGS scientist Jeffrey Lovich and Maryville College ...
... oil spill in the Gulf of Mexico, the U.S. ... Engineering and National Research Council to convene a committee ... disaster and identify measures for preventing similar harm in ... IMPROVING OFFSHORE DRILLING SAFETY, the committee,s final report, analyzes ...
... believe decision making mechanisms in the human brain could mirror ... similarities have been found in decision making systems between humans ... bees could teach us about how our brains work. ... an often paralysing thought process in humans, with scouts who ...
Cached Biology News:Literature review on solar energy and wildlife impacts research 2Swarms of bees could unlock secrets to human brains 2
(Date:7/24/2014)... 24, 2014 -- A novel combination of microscopy and ... Energy,s Oak Ridge National Laboratory an unprecedented look at ... physical and electrochemical properties. , The research team ... the surface of a perovskite manganite, a complex material ... avenue to understand surface behavior could benefit researchers who ...
(Date:7/24/2014)... 24, 2014  Now available is a stem cell ... Regenerative Therapies , located in Guadalajara, Mexico ... people a new health option: stem cell therapies ... countless patients suffer from joint and muscle injuries due ... tears, torn rotator cuff, tennis elbow, and knee injuries. ...
(Date:7/24/2014)... Calif. , July 24, 2014  ECC West ... Denver, Colorado with University College Hospital (UCH) ... a development project to finance, design, construct, and operate ... Nigeria . Under the first of ... prepare scoping documents and funding applications. UCH Ibadan intends ...
(Date:7/24/2014)... A research team led by group leader Yung-Eun ... technology to synthesize sulfur-doped and nitrogen-doped graphenes which ... secondary batteries and fuel cells. Yung-Eun Sung is ... Nanoparticle Research at Institute for Basic Science* (IBS) ... , This achievement has great significance with regards ...
Breaking Biology Technology:ORNL study reveals new characteristics of complex oxide surfaces 2Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2
... present the 2011 "ALBERT EINSTEIN" World Award of Science ... Distinguished University Professor University of Toronto, Canada. ... Ozin as the winner of the 2011 "ALBERT EINSTEIN" ... the field of nanochemistry that have helped to define ...
... Pharmaceuticals, Inc. (Nasdaq: ANDS ) is updating the ... Nicolaus Healthcare Conference to Thursday, September 8, 2011 at 4:25 ... at the Four Seasons Hotel in Boston.  Steve Worland, Ph.D., ... overview of Anadys and its clinical development programs, setrobuvir and ...
... ALCLS), the French genome engineering specialist, will present at the UBS ... 2011, and at the MidCap Event in Paris on September 22 ... the activities and the development strategy of the Cellectis group at ... York hotel on Tuesday, September 20, 2011 at 11:30 am ET. ...
Cached Biology Technology:2011 Awards of World Cultural Council 22011 Awards of World Cultural Council 32011 Awards of World Cultural Council 42011 Awards of World Cultural Council 5Anadys Pharmaceuticals Updates Its Presentation Date at the Stifel Nicolaus Healthcare Conference 2
... a comprehensive array of accessories. JULABO ... seamless, splash-water protected keypad offer a high ... up to 6 temperature values is visible ... with PID temperature control eliminates temperature overshoot ...
... HRP Conjugate Stabilizer is an aqueous solution ... other non-toxic stabilizing chemicals in a MOPS ... 6.7. This product contains a combination ... ppm Proclin 300 (Rohm and Haas Company) ...
Request Info...
... FLA-7000 excels at fluorescent and radioisotopic image ... features four easy-to-access filters (filters of Y520, ... Imaging Plates) for upgrading flexibility. In addition, ... four kinds of excitation laser beams. ,The ...
Biology Products: