Navigation Links
Largest synthetic gene ever built offers insights into anti-malarial drug resistance

ion with investigators at the National Institutes of Health, described in 2000 that the PfCRT gene is primarily responsible for the vast majority of drug resistance that leads to human death from malaria. Earlier this year, they also helped describe how the gene PfNHE likely contributes, and this turns out not to be as vital as PfCRT. Now, based in part on this study, they know that PfMDR1 is dependent on mutated PfCRT, and probably doesn't act independently.

"The contribution of PfMDR1 to anti-malarial drug resistance is important, but much smaller than what many researchers thought it would be," said Roepe. "Before the discovery of PfCRT, many thought PfMDR1 would be the primary culprit."

Roepe, and his two other co-authors, Georgetown researchers Linda Amoah, PhD, and Jacqueline Lekostaj, an MD/PhD student at Lombardi Comprehensive Cancer Center, employed an unusual method to construct the PfMDR1 gene. They first obtained the PfMDR1 protein sequence from the NIH PubMed data base. They then "back translated" the protein sequence using a computer program to change the parasite gene sequence − which combines nucleotide base pairs in a way that is found in no other life form − into one that could be "read" by yeast. The yeast are then able to produce the PfMDR1 protein, in its proper sequence, from this synthetic gene. They previously used this same technique to create and express a synthetic version of the much smaller PfCRT gene. PfMDR1 is made up of about 4,000 base pairs and its protein is particularly large, Roepe said, making the recent work much more challenging.

Already, analysis of these three anti-malarial drug resistance genes is painting a picture of how the parasite escapes destruction by most of the agents now used against it, he said.,

When a malaria parasite is transmitted through a mosquito bite, the mosquito injects the parasite into skin or blood, which then travels to the liver within minutes, grows
'"/>

Source:Georgetown University Medical Center


Page: 1 2 3 4

Related biology news :

1. Largest computational biology simulation mimics life’s most essential nanomachine
2. Largest study of human interactome reveals a novel way
3. Largest genomic search finds genes that may contribute to autism
4. Inexpensive, mass-produced genes core of synthetic biology advances at UH
5. Researchers find promising cancer-fighting power of synthetic cell-signaling molecule
6. Tagging pathogens with synthetic DNA barcodes
7. Breakthrough in micro-device fabrication combines biology and synthetic chemistry
8. Heparin prepared synthetically could replace animal-derived drug
9. Williams College biologist explores photosynthetic apparatus
10. New synthetic self-assembling macromolecules mimic nature
11. Locusts built-in surface analysis ability directs them to fly overland
Post Your Comments:
(Date:7/31/2015)... 31, 2015 The 10 th International Conference ... BGI from October 22-25, 2015, in Shenzhen , ... is celebrating its 10 th anniversary this year. Since its ... most influential annual meetings in the ,omics, fields, and is ... ICG-10 focuses on recent breakthroughs and advances ...
(Date:7/23/2015)... 23, 2015 Aware, Inc. (NASDAQ: AWRE ), ... financial results for its second quarter ended June 30, 2015.  ... was $4.5 million, a decrease of 33% compared to $6.8 ... the second quarter of 2015 was $0.3 million, or $0.01 ... per diluted share, in the same period a year ago.  ...
(Date:7/21/2015)... Passwords have proven futile for securing ... U.S. Office of Personnel Management breach. Biometric authentication ... but developers and end-users are concerned about how ... Biometrics-as-a-Service provider HYPR Corp. announced today the general ... party integration. A video overview ...
Breaking Biology News(10 mins):The 10th International Conference on Genomics (ICG-10) to Open in October 2Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6HYPR Corp. Launches Industry First Biometric Tokenization Platform 2HYPR Corp. Launches Industry First Biometric Tokenization Platform 3HYPR Corp. Launches Industry First Biometric Tokenization Platform 4
... of building a complete, functional, artificial cell. Though this ... many are making progress on various fronts. Prof. Roy ... Material,s and Interfaces Department recently took a significant step ... system on a glass chip. This system, composed of ...
... Researchers Robert Hoare ( Landcare Research , New Zealand) and Erik ... moths after the Minotaur of Greek mythology and the ... of southern pigmy moths . The study was published in ... tiny moths, with wingspans of 3 to 8 millimetres, belong to ...
... the functions of the three enzymes Tan, Ebony and Black ... in neurotransmitter recycling for the visual process. RUB researchers from ... flies cannot see without this recycling. Their analysis of the ... Anna Ziegler, Florian Brsselbach and Bernhard Hovemann report in the ...
Cached Biology News:Cell on a chip reveals protein behavior 2Tiny minotaurs and mini-Casanovas: Ancient pigmy moths reveal secrets of their diversity 2Blind flies without recycling 2Blind flies without recycling 3
(Date:7/30/2015)...  With over 60,000 customers across the globe, ISN improves the efficiency and effectiveness ... more than 60,000 safe and reliable contractors in over 80 countries. ... ... Over 60,000 Worldwide Customers In ... Headquartered in Dallas, TX , ISN has additional ...
(Date:7/30/2015)... July 30, 2015 HIGHLIGHTS:Q2 2015 ... 2014) , Reported sales were $697 million compared ... Sales grew organically by 8%, and changes in foreign ... increased sales by 1%. , By business unit, ... Applied and 11% in SAFC Commercial. , Reported ...
(Date:7/30/2015)... ... July 30, 2015 , ... As part of its 2015 growth plan and as a ... technical service business units, Whitehouse Laboratories is pleased to announce that it has begun ... Container Center of Excellence and will be strictly dedicated to basic USP 51, USP 61, ...
(Date:7/29/2015)... , July 30, 2015 ... reports results for the second quarter of 2015. ... group,s results and its performance in different businesses. ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Topics ... Q2 results - Performance drivers ...
Breaking Biology Technology:ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 2Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 4Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 5Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 6Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 7Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 8Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 9Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 10Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 11Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 12Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 13Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 14Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 15Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 16Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 17Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 18Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 19Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 20Whitehouse Labs Formally Announces Plans to Open a Microbiological Laboratory 2
... RALEIGH, N.C., April 3, 2008 Campbell Alliance, ... and,biotech industries, today announced that its Managed Markets ... Bio/Pharmaceutical Forum on Effective,Product Pricing and Modeling Strategies ... conference will be held on April 10 through ...
... April 3, 2008 Dendreon Corporation,(Nasdaq: DNDN ) ... with an institutional investor to sell 8.0 million shares ... 8.0 million shares of its,common stock for gross proceeds ... estimated offering expenses, in a,"registered direct" offering. The investor ...
... Offering Broad Array of Healthcare Products to ... with Immune Deficiency and Bleeding Disorders, OTTAWA, ... today,announced that Canadian Blood Services, and Hema-Quebec, have ... (Antihemophilic Factor,[Recombinant]), Humate(R) P (Antihemophilic factor / von ...
Cached Biology Technology:Campbell Alliance Managed Markets Practice to Take a Leadership Role at the CBI Forum on Effective Product Pricing and Modeling Strategies 2Campbell Alliance Managed Markets Practice to Take a Leadership Role at the CBI Forum on Effective Product Pricing and Modeling Strategies 3Dendreon Announces $47 Million Registered Direct Offering 2Dendreon Announces $47 Million Registered Direct Offering 3CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 2CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 3CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 4CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 5CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 6CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 7CSL Behring Signs Agreements With Canadian Blood Services and Hema-Quebec to Supply Life-Saving Therapies 8
Bovine Brain Microvascular Endothelial Cells (BBMVEC) (>500,000 cells)...
... activated ,• Eliminates nonspecific products due ... Excellent specificity allows extension through DNA regions ... Leaves an 'A' overhang ,TEMPase ... GeneChoice DNA Polymerase that remains inactive ...
Rabbit anti-CRSP1 (TRAP220), Polyclonal Antibody, Unconjugated Applications: Immunohistochemistry - 1:100 - 1:500...
... Probes is pleased to offer the ... ultrasensitive detection of primary amines. Developed ... University, the ATTO-TAG reagents are similar ... naphthalene-2,3-dicarboxaldehyde (NDA) and anthracene-2,3-dicarboxaldehyde, all of ...
Biology Products: