Navigation Links
Key mechanism in genetic inheritance during cell division identified

A key mechanism in the passing of genetic material from a parent cell to daughter cells appears to have been identified by a team of Berkeley researchers. Their study may explain how a complex of proteins, called kinetochores, can recognize and stay attached to microtubules, hollow fibers in the walls of biological cells that are responsible for the faithful segregation of chromosomes during cell division.

"In test tube experiments, we've found that the kinetochore proteins form rings around the microtubules and this ring formation promotes microtubule assembly, stabilizes against disassembly, and promotes bundling," says Eva Nogales, a biophysicist who holds joint appointments with the Lawrence Berkeley National Laboratory (Berkeley Lab), the University of California at Berkeley, and the Howard Hughes Medical Institute (HHMI). "If ring formation takes place in vivo, it could be the mechanism by which chromosomes are kept segregated during mitosis."


Nogales is one of the co-authors of a paper reporting the results of this research which appears in the January 21, 2005 issue of the journal Molecular Cell. Other authors of the Molecular Cell paper were Georjana Barnes, David Drubin and Stefan Westermann, with UC Berkeley's Department of Molecular and Cell Biology, who were the lead investigators on this work, plus Agustin Avila-Sakar and Hong-Wei Wang, with Berkeley Lab, and Hanspeter Niederstrasser and Jonathan Wong with UC Berkeley.

Says Barnes, "Mistakes in chromosome segregation during mitosis contribute to cancer and birth defects. From various genetic experiments we know that the activity of a 10-protein complex of kinetochores, called Dam1, is responsible for the faithful segregation of chromosomes during mitosis. While we don't know at this time that ring formation occurs in vivo, we do see in our in vitro tests that Dam1 ring formation strengthens the microtubules."

Thousands of microtubule fibers are woven togethe r to form a highly flexible cytoskeleton in biological cells that gives shape to cell walls and other structures, and controls the transportation of substances in and out of a cell. During cell division (mitosis), the microtubule fibers disassemble and reform into spindles across which duplicate sets of chromosomes line up. During this phase, it is critical that the spindles maintain their structural integrity so that they can segregate a single copy of each chromosome to each daughter cell. After which, the microtubules again disassemble and reform back into skeletal systems for the two new daughter cells. It has been determined, through the genetic research of Barnes and Drubin, among others, that kinetochores must bind to a microtubule spindle to avoid the gain or loss of chromosomes by each of the daughter cells. How this works, however, was unknown.

To find answers, the Berkeley researchers used a purified, reconstituted Dam1 complex, obtained from genetically engineered E. coli bacteria, and compared its effects on microtubules in vitro to the effects caused by certain Dam1 mutants. Their analysis shed new light on the structural nature of the kinetochore-microtubule interface, and may provide a biochemical explanation for the role of kinetochores in maintaining chromosome segregation during mitosis.

"Our studies indicate that the Dam1 rings are formed by longitudinal self-assembly of multiple copies of the Dam1 complex upon the microtubule surface," the authors state in their Molecular Cell paper. "Although the presence of microtubules strongly facilitates the oligomerization process, ring assembly seems to be an intrinsic property of the Dam1 complex, as we have been able to induce self-assembly into rings in the absence of microtubules."

The formation of rings around the microtubules by the purified Dam1 complex that the Berkeley collaborators observed has not been reported for any other microtubule binding protein complex. While the authors say that ring formation is a complicated way to construct a microtubule binding structure, a microtubule binding ring might be uniquely suited to fulfill the functions of the Dam1 complex. In comparison with the purified Dam1, the Dam1 mutants produced partially formed rings that reduced microtubule binding.

"Because the stability of a microtubule is thought to be largely governed by the lateral interactions between adjacent protofilaments," the authors note, "rings that bind orthogonally to the microtubule axis are not only expected to strengthen interprotofilament interactions, but to also prevent protofilament peeling, which in turn would encourage further growth.

Furthermore, the Berkeley researchers showed that binding between the ring and the microtubule is mediated by flexible domains, and is largely electrostatic in nature. This could allow for lateral sliding or diffusion of Dam1 rings along microtubules. Analysis of the Dam1 rings after disassembly of microtubules, revealed an accumulation of rings at the ends of the microtubule, suggesting that the rings do not disassemble but slide back as the microtubule protofilaments peel away from the ends.

Says Nogales, "That the rings remain attached to the microtubule end as it depolymerizes, is a most ingenious mechanism to move chromosome to the two daughter cells during anaphase, without even requiring energy. This is great finding!"


'"/>

Source:Eurekalert


Related biology news :

1. A new mechanism of regulating RNA degradation
2. Plants, animals share molecular growth mechanisms
3. PET/MRI scans may help unravel mechanisms of prenatal drug damage
4. Researchers discover molecular mechanism that desensitizes us to cold
5. Chemists identify immune system mechanism for methamphetamine binges
6. Researchers unlock mechanism creating jigsaw puzzle-like plant cells
7. Mouse gene shows new mechanism behind cardiac infarction in man
8. UCSD research reveals mechanism involved with type of fatal epilepsy
9. Ancient immune defense mechanism is no match for HIV-1
10. Malaria mechanism revealed
11. Researchers discover mechanism for multiplying adult stem cells
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/22/2016)... PROVO and SANDY, ... Screening Ontario (NSO), which operates the highest sample volume ... testing, and Tute Genomics and UNIConnect, leaders in clinical ... today announced the launch of a project to establish ... testing panel. NSO has been contracted ...
(Date:3/18/2016)... --> --> ... & Unmanned Vehicles, Physical infrastructure and Perimeter Surveillance & Detection ... border security market and the continuing migration crisis in the ... has led visiongain to publish this unique report, ... defence & security companies in the border security ...
(Date:3/15/2016)... March 15, 2016 --> ... by Transparency Market Research "Digital Door Lock Systems Market - ... - 2023," the global digital door lock systems market in ... 2014 and is forecast to grow at a CAGR of ... small and medium enterprises (MSMEs) across the world and high ...
Breaking Biology News(10 mins):
(Date:5/24/2016)... May 24, 2016   MedyMatch Technology Ltd ., the ... intelligence, real-time decision support tools in the emergency room, announced ... 2016 Israeli Advanced Technology Industries (IATI) BioMed Conference. ... 15th National Life Sciences and Technology Week, and ... Intercontinental Hotel in Tel Aviv, Israel . ...
(Date:5/23/2016)... WARSAW, Ind. , May 23, 2016 Zimmer ... in musculoskeletal healthcare, today announced that its Board of Directors ... stockholders for the second quarter of 2016. ... on or about July 29, 2016 to stockholders of record ... Future declarations of dividends are subject to approval of the ...
(Date:5/22/2016)... NC (PRWEB) , ... May 22, 2016 , ... Doctors ... weapons in combating the asbestos cancer, malignant mesothelioma. Surviving Mesothelioma has just posted an ... Researchers in the University of Rome’s Department of Clinical Sciences and Translational Medicine evaluated ...
(Date:5/19/2016)... ... 2016 , ... Anton Paar USA, located in Ashland, Virginia is pleased to ... new structure adds a third office building to the current facilities. , Growth ... of land, along with office space adjacent to the previous main building. Through remodels ...
Breaking Biology Technology: