Navigation Links
Jefferson researchers building a better rabies vaccine

In an unexpected discovery, scientists at Jefferson Medical College have found that a tiny change in a rabies virus protein can turn a "safe" virus extremely deadly. The finding has enabled the researchers to refine a vaccine they previously created against rabies in wildlife, making it safer and more effective.

"We have identified a molecular mechanism involved in making the rabies virus pathogenic and lethal, and have developed a very safe vaccine for the immunization of wildlife," says Bernhard Dietzschold, DVM, professor of microbiology and immunology at Jefferson Medical College of Thomas Jefferson University in Philadelphia, who led the work. "As a result, we think we have engineered a virus which is nonpathogenic and extremely stable."

Reporting recently in the Journal of Virology, Dr. Dietzschold, Matthias Schnell, Ph.D., associate professor of microbiology and immunology at Jefferson Medical College, and their co-workers identified a mutation in a rabies virus gene that changes a single amino acid in the virus's outer coat. The alteration was enough to turn a "safe" virus into a deadly version, making the virus unusable for vaccination.

Wildlife rabies is a substantial health problem in the United States. It is particularly prevalent along the East Coast, where more than 90 percent of reported cases occur in wildlife. Raccoons are the most affected, with skunks a close second. Worldwide--and especially in underdeveloped nations--rabies takes a large human toll; more than 60,000 deaths a year. In the United States alone, more than $1 billion is spent annually for control, treatment and prevention of rabies.

The Jefferson scientists uncovered the mutation, during the testing of a virus they thought was suitable for use in vaccinating wildlife. The virus is first altered so that it will not cause disease, yet will arouse the immune system's defenses. Because viruses such as rabies have high mutation rates, researchers typically " passage" the virus through mice. That is, they inject it into the animals, recover it, and then put into a second mouse. This is repeated at least five times. Five generations, Dr. Dietzschold explains, is usually enough to see whether the virus will either mutate and turn dangerous, or show genetic stability.

The research team has tested the current virus in 10 passages in mice, and it remained safe while retaining its potency. Dr. Dietzschold notes that the likelihood of a mutation occurring is extremely low. While different varieties of rabies virus exist, they maintain enough similarities in the all-important outer glycoprotein, that a vaccine that takes advantage of immune reactions to this protein can be effective.

Dr. Dietzschold explains that the current wildlife rabies vaccine used in the United States is ineffective in a chief carrier: skunks. The vaccine developed by his team is much broader, showing efficacy in such animals as raccoon, skunk and mongoose. One of the problems with current vaccines is the fact that several varieties are used, depending on the particular species of animal. The scientists hope their vaccine will prove useful for rabies prevention in several species.

The newer virus and vaccine currently are being evaluated by Molecular Targeting Technologies, Inc., of West Chester, Pa. The researchers are asking for permission from the United States Department of Agriculture and the Environmental Protection Agency to perform field trials. These would entail placing food baits containing the vaccine in the wild, then later capturing and testing animals for antibodies against the rabies virus.


'"/>

Source:Thomas Jefferson University


Related biology news :

1. Jefferson Virologists Coax HIV Out of Hiding
2. Jefferson researchers find nanoparticle shows promise in reducing radiation side effects
3. Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method
4. Jefferson scientists identify gene defect leading to abnormal skin development and cancer
5. Jefferson scientists identify gene mutation potentially involved in breast cancer initiation
6. Immune cell communication key to hunting viruses, Jefferson immunologists show
7. Jefferson researchers uncover new way nature turns genes on and off
8. Jefferson scientists find aging gene also protects against prostate cancer development
9. Jefferson scientists find high glucose before surgery raises risk of dangerous complications
10. Jefferson researchers discovery may change thinking on how viruses invade the brain
11. Elderly spinal cord injuries increase five-fold in 30 years, Jefferson neurosurgeons find
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
(Date:5/20/2016)... 20, 2016  VoiceIt is excited to announce ... By working together, VoiceIt and VoicePass ... and VoicePass take slightly different approaches to voice ... security and usability. ... new partnership. "This marketing and technology ...
(Date:5/9/2016)... 9, 2016 Elevay is currently ... expanding freedom for high net worth professionals seeking travel ... globally connected world, there is still no substitute for ... duplicate sealing your deal with a firm handshake. This ... taking advantage of citizenship via investment programs like those ...
Breaking Biology News(10 mins):
(Date:6/27/2016)...  Global demand for enzymes is forecast to ... $7.2 billion.  This market includes enzymes used in ... production, animal feed, and other markets) and specialty ... and beverages will remain the largest market for ... products containing enzymes in developing regions.  These and ...
(Date:6/27/2016)...  Sequenom, Inc. (NASDAQ: SQNM ), a ... the development of innovative products and services, announced today ... States denied its petition to review decisions ... U.S. Patent No. 6,258,540 (",540 Patent") are not patent ... Supreme Court,s Mayo Collaborative Services v. Prometheus Laboratories decision.  ...
(Date:6/27/2016)... CA (PRWEB) , ... June 27, 2016 , ... ... for clinical trials, announced today the Clinical Reach Virtual Patient Encounter CONSULT ... care circle with the physician and clinical trial team. , Using the CONSULT module, ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. ... the faculty of the University of North Carolina Kenan-Flagler Business School ... entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s international efforts, leading ...
Breaking Biology Technology: