Navigation Links
Interfering RNA silences genes in 'slippery' immune cells

o extend RNAi to a wider pool of researchers by making the technique less expensive and more widely available, as well as adapting it to T cells, a cell type previously intractable to such manipulation. Their technique combines three technologies already accessible to lab investigators: nucleofection, siRNA expression cassettes, and siRNA expression vectors. Nucleofection technology uses specialized solutions and electrical pulses to temporarily open a cell nucleus. Into the nucleus, researchers insert a payload of DNA.

The DNA holds a sequence of genetic code that produces a specific siRNA after it enters a nucleus. The researchers encased the DNA within an siRNA expression cassette (SEC), an inexpensive, quickly synthesized product that carries genetic sequences to regulate the gene activity that yields an siRNA. After the researchers tested a variety of SECs to determine which is the most effective, they inserted the desired SEC into a vector, a biological agent that inserts itself into a target cell's nucleus more efficiently than an unaccompanied cassette.

The researchers first tested their approach by introducing a gene for green fluorescent protein into human T cells, and using siRNA to inhibit that gene's expression, and dim its fluorescent glow.

They then applied their approach to HALP, a gene naturally active in T cells. Dr. Finkel previously discovered and named HALP, an acronym for "HIV-associated life preserver," showing that it had a role in prolonging HIV infection by helping HIV-infected T cells survive attack by the immune system.

Using siRNA and their laboratory techniques, the investigators succeeded in "knocking down," that is, decreasing gene expression by HALP. Because their previous research strongly suggests that HALP promotes latent HIV infection, the new technique has a potential application to HIV treatment. "The siRNA may represent a suicide vector: by knocking down HALP it may allow HIV-infected cells
'"/>

Source:Children's Hospital of Philadelphia


Page: 1 2 3

Related biology news :

1. Used in a new way, RNA interference permanently silences key breast cancer gene
2. Researchers find protein that silences genes
3. MIT: Pulsing light silences overactive neurons
4. Newly-discovered class of genes determines ?and restricts ?stem cell fate
5. Inexpensive, mass-produced genes core of synthetic biology advances at UH
6. First atlas of key brain genes could speed research on cancer, neurological diseases
7. U-M scientists find genes that control growth of common skin cancer
8. Researchers find missing genes of ancient organism
9. Scientists document complex genomic events leading to the birth of new genes
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. Advances in the characterisation of the oyster mushroom genes
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Interfering RNA silences genes slippery immune cells

(Date:4/18/2014)... population predicted to reach 9 billion by 2050 ... considers human-altered landscapes such as farmland, according to ... that supports it might be an increasingly scarce ... of the land surface is directly affected by ... impacts such as climate change. But what if ...
(Date:4/18/2014)... comet impacts can cause widespread ecological havoc, killing off ... But new research from Brown University shows that impacts ... the time of an impact. , A research team ... of leaves and preserved organic compounds lodged inside glass ... material could provide a snapshot of environmental conditions at ...
(Date:4/17/2014)... peptide inhibits RSV infection , Respiratory syncytial ... respiratory tract infections, generating life-threating illness in very ... therapies are limited. RSV enters host cells through ... six-helix fusogenic bundle. Small interfering peptides that prevent ... these peptides are highly susceptible to degradation. In ...
Breaking Biology News(10 mins):Stanford researchers rethink 'natural' habitat for wildlife 2Stanford researchers rethink 'natural' habitat for wildlife 3Stanford researchers rethink 'natural' habitat for wildlife 4Impact glass stores biodata for millions of years 2Impact glass stores biodata for millions of years 3JCI online ahead of print table of contents for April 17, 2014 2JCI online ahead of print table of contents for April 17, 2014 3JCI online ahead of print table of contents for April 17, 2014 4JCI online ahead of print table of contents for April 17, 2014 5JCI online ahead of print table of contents for April 17, 2014 6
... 2011 Robotics researchers at Ben-Gurion University of the Negev ... develop intelligent sensing and manipulation algorithms for robots that ... project is part of the European Union Seventh Framework ... will develop the scientific know-how and several prototype systems ...
... more than 4 billion nucleotides, a draft sequence of the ... American Association for the Advancement of Science (AAAS). , The ... Prize annually recognizes the author or authors of an outstanding ... the journal Science between June and the following ...
... disorder that can lead to life-threatening bleeding episodes is ... findings from a new international research project led by ... it determines the treatment decision," says Maha Othman, a ... who led the three-year research project on the rare ...
Cached Biology News:Ben-Gurion U. part of EU consortium to develop robots that selectively pick fruit 2Draft sequence of Neandertal genome wins the 2010 AAAS Newcomb Cleveland Prize, supported by Affymetrix 2
(Date:1/15/2014)... 15, 2014 Freeslate, Inc ., ... announced that Lupin Limited, one of India’s top five ... Protégé PharmD System for high throughput solid form ... focused on a wide range of quality, affordable generic ...
(Date:1/15/2014)... Jan. 15, 2014 TaiGen Biotechnology Company, Limited ("TaiGen") ... with R-Pharm, a leading Russian pharmaceutical company, to develop ... Russian Federation , Turkey ... (CIS). Nemonoxacin is a novel antibiotic for the treatment of ...
(Date:1/14/2014)... DC (PRWEB) January 14, 2014 Carahsoft ... for Thursday, January 23, 2014 at 2pm EST (11am ... Knowledge.” The topic focuses on how technology can turn ... critical decisions for government agencies. The online webinar will ...
(Date:1/14/2014)... WASHINGTON , Jan. 14, 2014  3D Communications, a leading provider ... scientific, regulatory, business, and media events in the United ... its former associate Virginia Cox , JD, is returning to ... Virginia Cox re-joins 3D after more than two years ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2Former FDA Associate Commissioner Returns To 3D Communications 2
... CITY, Calif., Jan. 7 Abaxis, Inc. (Nasdaq: ... manufacturing point-of-care blood analysis instruments for both the ... has entered into a license agreement for co-exclusive ... diagnostics in the professional marketplace for certain technologies ...
... Jan. 7 News briefs concerning economic,and business growth in ... Draper Labs Acquires Manufacturing Site in ... Draper Laboratory bought a 41,000-square-foot building,in St. Petersburg recently where ... the building, 10050 16th St. N., should be completed,in March ...
... 6 /PRNewswire-Asia/ -- WuXi PharmaTech (Cayman) Inc.,(NYSE: ... medical device,research and development outsourcing company with operations ... Dr. Ge Li, the Company,s Chairman and,Chief Executive ... Morgan,Healthcare Conference on Tuesday, January 13, 2009, at ...
Cached Biology Technology:ABAXIS Announces Worldwide Animal Health Licensing Agreement for Rapid Test Technology 2ABAXIS Announces Worldwide Animal Health Licensing Agreement for Rapid Test Technology 3Highlights of Economic and Business Growth in the Tampa Bay Region (News Briefs) 2Highlights of Economic and Business Growth in the Tampa Bay Region (News Briefs) 3Highlights of Economic and Business Growth in the Tampa Bay Region (News Briefs) 4WuXi PharmaTech to Present at 27th Annual J.P. Morgan Healthcare Conference 2
See product name for description....
NATIVE, ENZYME PORCINE TRYPSIN...
Polyethylene Glycol 8000, 1 kg. PEG 8000.Used in differential DNA precipitation. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
G-Protein Coupled Receptor Assay, For 500 assays. SPA. Category: Drug Screening & Cellular Assays, Assays ....
Biology Products: