Navigation Links
Imaging techniques permit scientists to follow a day -- or four -- in the life of a cell

\The movement and growth of cells are critical for normal physiological processes, and--when perturbed--can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance. The current issue of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses these concerns with two freely accessible protocols: one for inserting 'reporter' proteins into cells to monitor what's going on inside, and another for maintaining the cells under a microscope for long-term observation.

The first protocol, available at http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4657, details a procedure to inject proteins into individual cells. The injected protein can be fluorescently labeled, so scientists can track the glowing protein with a microscope and observe what it's doing and where it's going inside the cell. This microinjection technique is similar to those that are used to create cloned and transgenic organisms, and it can also be applied to more complex assays--including laser photobleaching and fluorescent speckle microscopy--that yield specific insights into cellular processes.

A second freely available protocol (http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4660) outlines the construction and use of an enclosed microscope chamber. This device is designed to optimize the culture environment for cells--allowing them to grow as they would normally--while optimizing the conditions for viewing them by microscopy. This enables researchers to monitor the cells under a microscope for long time periods (in excess of four days), during which the cells may divide multiple times
'"/>

Source:Cold Spring Harbor Laboratory


Page: 1 2

Related biology news :

1. Imaging Lymph Nodes with Nanoparticles
2. Special Imaging Study Shows Failing Hearts Are Energy Starved
3. Imaging study links key genetic risk for Alzheimers disease to myelin breakdown
4. Imaging pinpoints brain regions that see the future
5. Newer imaging techniques may lead to over-treatment
6. Rice bioengineers pioneer techniques for knee repair
7. Evolutionary biology research techniques predict cancer
8. U of MN uses robotic surgery techniques in cardiac cell therapy research
9. Cloning techniques produce FDA-approved antibiotic
10. Multiple genes permit closely related fish species to mix and match their color vision
11. Wisconsin scientists grow critical nerve cells

Post Your Comments:
(Date:11/9/2009)..., , BIRMINGHAM, Ala. The U.S. Department of Ener...he University of Alabama at Birmingham (UAB) Schoo...educe greenhouse gas emissions through the capture...carbon dioxide (CO2). The project is in collaborat...labama Power. , Funded by the DOE through the 20...
(Date:11/9/2009)..., The brilliance of butterfly wings has inspire...s to deliver innovation in the fields of security,...ter and international technology company QinetiQ h...p new technologies based on groundbreaking physica...s a team of physicists from the University of Exet...
(Date:11/9/2009)..., PHILADELPHIA Scientists and industry leaders f...-19, 2009, for the AACR-NCI-EORTC Molecular Target..., which will feature groundbreaking information on...Cancer is a complex disease and to treat it effect...ity that takes place at the molecular level," said...
Breaking Biology News(10 mins):UAB, partners seek safe carbon dioxide storage for 'greener' power generation 2UAB, partners seek safe carbon dioxide storage for 'greener' power generation 3£3.2m ($5.4 million) research project to capture brilliance of butterfly wings 2Nuvilex Introduces Last Shot Hangover Remedy 28TM 29 Video 55546 1Nuvilex Introduces Last Shot Hangover Remedy 28TM 29 Video 55546 2The Cosmetic Bootcamp LLC Announces Novel Presentations at Its 3rd Annual Marketing and Management Symposium 55542 1White House Sued Over Free Speech Violations In Healthcare Battle 3A Doctor 26 Patient Groups Say White House Intended to Shut Up Opponents 55538 1White House Sued Over Free Speech Violations In Healthcare Battle 3A Doctor 26 Patient Groups Say White House Intended to Shut Up Opponents 55538 2
...ARY 23, 2009 The legacy of nuclear weapons and nu... sediment at dozens of sites across the United Sta...th uranium. The uranium is transported through gro...trategy, uranium immobilization in contaminated gr...ition of organic molecules known as electron donor...
... light emission by living organisms, bioluminescen... It is known that light is generated by chemical r...nt part. In the animal world, these chemical react...d photocytes. These are aggregated into complex li...ulated by nerve impulses, and in which light can b...
... diet at the time we are conceived set the course ... at the heart of a new study based on an "experime...nded researchers. , We inherit our DNA the genet... parents: 50% of our DNA from our mothers and 50% ...on, deletion or duplication of information, this D...
Other Biology News:Patience pays off with methanol for uranium bioremediation 2New light shed on marine luminescence 2'Experiment of nature' examines how mother's diet may impact on child's health 2'Experiment of nature' examines how mother's diet may impact on child's health 3
(Date:11/6/2009).... 6 BioSpecifics Technol...company developing first-in-class collagenase-base...for the third quarter ended September 30, 2009. ,...f this year, the FDA,s Arthritis Advisory Committe...approval of XIAFLEX(TM) for the treatment of Dupuy...
(Date:11/6/2009)..., November 6 EUCODIS Bioscience, a...nd marketing enzymes for the,chemical industry ann...greement with Bio Sidus, the leading biopharmaceut...ement gives EUCODIS Bioscience exclusive access - ...us, proprietary collection of,psychrophilic bacter...
(Date:11/6/2009)...Newswire-FirstCall/ - Oncolytics Biotech Inc. (TSX... today announced updated results from a Phase II s...arcomas metastatic to the lung in a poster present...y Society Meeting held in Miami Beach, Florida, fr...ntitled "A Phase II Study of Intravenous REOLYSIN ...
(Date:11/5/2009)...ne hybrid . . . , Fuel economy ratings for the... the www.fueleconomy.gov web site, which Oak Rid... the U.S. Department of Energy and the U.S. Enviro...cles on the best fuel economy list are hybrid elec...ghway). "This list proves that hybrids have gone m...
Breaking Biology Technology:Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 2Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 3Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 4Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 5Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 6EUCODIS Bioscience to Explore Novel Enzymatic Activities in Antarctic Bacteria Library From Bio Sidus 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 2November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 3
...,(Nasdaq: MAXY ), a biotechnology company focused...ay announced that it will present at the,JPMorgan ...ry 7, 2008 at,9:00 a.m. Pacific Time. Russell Howa...Vetticaden, Maxygen,s chief medical officer, will ... accessed on Maxygen,s website at, http://www.maxy...
...ber 28, 2007 at 8:30 a.m ...xza,Pharmaceuticals, Inc. (Nasdaq: ALXA ) today a...opment partnership with Endo Pharmaceuticals Holdi...fentanyl) and the fentanyl,class of molecules for ...opment, AZ-003 is a hand-held drug device that use...
...Science from Faux Science, NORTH KINGSTOWN, R.I.,...Society, Dominion Diagnostics sponsored an extensi...eview of Urine Drug Testing:,Which Test, Which Pat...ence," presented by Dr. Robert Barkin, Pharm.D, MB...r, Faculty of Anesthesiology, Family,Medicine, and...
Other Biology Technology:Alexza Announces Partnership With Endo for AZ-003 (Staccato(R) fentanyl) in North America 2Alexza Announces Partnership With Endo for AZ-003 (Staccato(R) fentanyl) in North America 3Alexza Announces Partnership With Endo for AZ-003 (Staccato(R) fentanyl) in North America 4Alexza Announces Partnership With Endo for AZ-003 (Staccato(R) fentanyl) in North America 5Arizona Pain Society and Dr. Robert Barkin, Rush University Medical Center, Present Program on Comprehensive Urine Drug Testing Sponsored by Dominion Diagnostics 2
iEMS thermal microplate holder from Thermo Scientific
Deluxe CO2 Backup, Factory Installed, All Voltages, for 3.0 and 6.7 cu ft -86C Chest Freezers & all -40C/ -86C Upright Freezers from Thermo Scientific
LN2 Backup, Factory Installed, All Voltages, for 3.0 and 6.7 cu ft -86C Chest Freezers & all -40C/ -86C Upright Freezers from Thermo Scientific
...se H- Reverse Transcriptase (RT) is an improved ve... a DNA polymerase that synthesizes a complementary...:DNA hybrid. This enzyme is genetically engineered...n a deletion in the RNase H active center. Like Su...
Biology Products: