Navigation Links
Imaging techniques permit scientists to follow a day -- or four -- in the life of a cell

\The movement and growth of cells are critical for normal physiological processes, and--when perturbed--can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance. The current issue of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses these concerns with two freely accessible protocols: one for inserting 'reporter' proteins into cells to monitor what's going on inside, and another for maintaining the cells under a microscope for long-term observation.

The first protocol, available at http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4657, details a procedure to inject proteins into individual cells. The injected protein can be fluorescently labeled, so scientists can track the glowing protein with a microscope and observe what it's doing and where it's going inside the cell. This microinjection technique is similar to those that are used to create cloned and transgenic organisms, and it can also be applied to more complex assays--including laser photobleaching and fluorescent speckle microscopy--that yield specific insights into cellular processes.

A second freely available protocol (http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4660) outlines the construction and use of an enclosed microscope chamber. This device is designed to optimize the culture environment for cells--allowing them to grow as they would normally--while optimizing the conditions for viewing them by microscopy. This enables researchers to monitor the cells under a microscope for long time periods (in excess of four days), during which the cells may divide multiple times and cycle through nearly all cellular functions. When coupled with the latest in image-analysis software, this technique allows researchers to gain a long-term perspective on events in the lives of cells.
'"/>

Source:Cold Spring Harbor Laboratory


Related biology news :

1. Imaging Lymph Nodes with Nanoparticles
2. Special Imaging Study Shows Failing Hearts Are Energy Starved
3. Imaging study links key genetic risk for Alzheimers disease to myelin breakdown
4. Imaging pinpoints brain regions that see the future
5. Newer imaging techniques may lead to over-treatment
6. Rice bioengineers pioneer techniques for knee repair
7. Evolutionary biology research techniques predict cancer
8. U of MN uses robotic surgery techniques in cardiac cell therapy research
9. Cloning techniques produce FDA-approved antibiotic
10. Multiple genes permit closely related fish species to mix and match their color vision
11. Wisconsin scientists grow critical nerve cells

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/3/2016)... Calif. , Feb. 3, 2016 Vigilant ... Police Department in Missouri ... license plate reader (LPR) data from Vigilant Solutions. ... hit-and-run case in which the victim was walking out of a convenience ... parking space next to his vehicle, striking his vehicle ...
(Date:2/2/2016)... 2016 This BCC Research report provides ... reviewing the recent advances in high throughput ‘omic ... field forward. Includes forecast through 2019. ... and opportunities that exist in the bioinformatic market. ... as well as IT and bioinformatics service providers. ...
(Date:2/2/2016)... 2016   Parabon NanoLabs (Parabon) announced ... Research Office and the Defense Forensics and Biometrics ... the company,s Snapshot Kinship Inference software ... generally, defense-related DNA forensics.  Although Snapshot is best ... and ancestry from DNA evidence), it also has ...
Breaking Biology News(10 mins):
(Date:2/3/2016)... Sunnyvale, CA (PRWEB) , ... February 03, 2016 ... ... publisher of remote Linux and Unix visualization solutions today announced the addition of ... Session Preview allows users to see the current state of the remote Linux ...
(Date:2/3/2016)... -- Silk Therapeutics, Inc., today announced the closing of a $6 ... a total of $10.25 million in Series A funding based ... round was led by existing investor The Kraft Group of ... investors Lear Corporation and Highland Consumer Partners, as well as ... Richard Sackler , MD, with Summer Road, LLC; Erin ...
(Date:2/3/2016)... ... February 03, 2016 , ... ... aid in the rapid development and ongoing quality control of molecular assays targeting ... is extremely high,” Dr. Gregory R. Chiklis, President and CEO of ZeptoMetrix, relayed ...
(Date:2/3/2016)... ... February 03, 2016 , ... Marktech Optoelectronics, a ... wafers, and InP epi wafers based in Latham, New York, offers a ... and Avalanche photodiodes–to Si and InGaAs PIN photodiodes. But it is Marktech's newly ...
Breaking Biology Technology: