Navigation Links
Image of myosin-actin interaction revealed in cover story of Molecular Cell

y hydrolysis cycles. The other myosins grab on tightly to actin and release after one hydrolysis cycle.

"This study required a different way of thinking about image analysis. This is the first time we were able to structurally visualize the weak binding states of actin and myosin, not interpolated from crystal structures, and not interpolated from biophysical methods," said Dr. Hanein. "We were able to see structural changes in the myosin lever arm as well as in the actin interface as it propagates through the hydrolysis cycle."

Structural information from previous studies provided information about parts of this process, but until the present collaboration, visualizing Myosin V in its weakly bound state to actin had not been possible. The Hanein group captured snapshots of Myosin V at several points during a hydrolysis cycle. Their use of electron cryo-microscopy made it possible to visualize flexible structural domains, which tether the Myosin V, helping to keep the protein on its actin track through the weak binding phase of the processive movement.

The detailed molecular knowledge of how myosin interacts through the hydrolysis cycle with actin provides an exciting new research template onto which scientists can design new sets of experiments to further refine the myosin-actin binding region and to correlate it with loss or gain of function. The precise characterization of this myosin-actin interface is critical, evident by the way a single amino acid change in myosin leads to familial hypertrophic cardiomyopathy (FHC), an undetectable condition resulting in death by sudden cardiac arrest in otherwise healthy young adults.

Contributors to this work include: Niels Volkmann, Ph.D., assistant professor and first author on this publication, Dorit Hanein, Ph.D., associate professor, Hong-Jun Liu and Larnele Hazelwood from the Burnham Institute for Medical Research; and Kathleen M. Trybus, Ph.D., Susan Lowey, Ph.D., and Elena B. Kremen
'"/>

Source:Burnham Institute


Page: 1 2 3

Related biology news :

1. Virus-host interactions at sea effect global photosynthesis
2. Future diabetes drugs may target new protein interaction
3. Computational verification of protein-protein interactions by orthologous co-expression
4. Confirmation of human protein interaction data by human expression data
5. Physical and functional interaction of key cell growth molecules linked to cancer
6. Complex gene interactions account for autism risk
7. A real time look at interactions between RNA and proteins
8. U of MN researchers develop way to visualize synchronized interactions of nerve cells in the brain
9. Biologists visualize protein interaction that may initiate viral infection
10. Biologists develop genome-wide map of miRNA-mRNA interactions
11. Mans best friend: Study shows lonely seniors prefer playtime with pooch over human interaction
Post Your Comments:
(Date:10/16/2014)... new synthesis of recent research findings to inform their ... two states. , The Ecology and Management of ... Synthesis of the Relevant Biophysical Science and Implications for ... the U.S. Forest Service,s Pacific Northwest Research Station, is ... a synthesis of the large body of scientific information ...
(Date:10/16/2014)... NXT-ID, Inc. (NASDAQ: NXTD ... company focused on the growing mobile commerce market announces ... the Smart Wallet, at the 13 th annual ... global conference on the intersection of technology and the ... , founder and publisher of the Strategic News Service™, ...
(Date:10/15/2014)... Ebola virus is spreading rapidly and to an unexpected ... in the past and the virus shows a new ... recorded before. For this reason, the German National Academy ... Science and Engineering, and the Union of the German ... on the Ebola epidemic today. , In the statement ...
Breaking Biology News(10 mins):New report synthesizes best available science on management of moist mixed-conifer forests 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5
... the extinction of the Javan rhinoceros in Vietnam pushes the ... creatures living in Indonesia., "We still have a chance to ... to determine the profile of the remaining group," he says., ... demise of the Javan rhinoceros population living in Vietnam by ...
... testing a way to determine the status of fetal ... and their babies. Supported by a two-year, $418,000 ... of Child Health and Human Development of the National ... cells for genetic study within the first two months ...
... on managing flows in streams and rivers or on ... Instead, watersheds need a good mix of steep, cold-running ... keep options open for salmon adapted to reproduce better ... ,Preserving that sort of varied landscape serves not just ...
Cached Biology News:Wayne State researchers working to improve genetic analysis, disorder detection 2Bears, scavengers count on all-you-can-eat salmon buffet lasting for months 2Bears, scavengers count on all-you-can-eat salmon buffet lasting for months 3
(Date:10/22/2014)... 21, 2014 The Americas Inorganic ... refrigerants market in Americas with analysis and forecast ... the Americas Inorganic Refrigerants Market report, to get ... also provides a glimpse of the segmentation in ... by various tables and figures. , ...
(Date:10/22/2014)... October 22, 2014 WriteResult, LLC ... – has been working with a team of researchers ... Haven Farms Community Farming Collaborative to provide electronic questionnaire ... The study, which started in May, aims to ... provides participants weekly exposure to and participation in urban ...
(Date:10/22/2014)... October 22, 2014 According to ... by Service Type (Consulting, Integration, Deployment & Support), ... Deployment Model (Public, Private, Others (Hybrid & Community)) ... defines and segments the Cloud Professional Services Market ... forecasting of revenues. It also identifies the drivers ...
(Date:10/22/2014)... , Oct. 22, 2014 Research ... "Organic Electronics Market by Material, by Application, Geography - ... to their offering. Organic electronics, also ... branch of material science which deals with small conductive ... organic because small molecules and polymers are carbon based, ...
Breaking Biology Technology:The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 2The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 3myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3
... SANTA CLARA, Calif., March 6 Finesse Solutions, LLC, ... solutions for life sciences process applications, announced the technical ... measurement system, at Interphex in New York on March ... single-use solution consisting of a disposable sheath, an optical ...
... Therapeutics, Inc. (CTI) (Nasdaq and MTA: CTIC) announced today ... Stock Market indicating that the NASDAQ Listing Qualifications Panel ... common stock on The NASDAQ Capital Market, subject to ... the Company demonstrate compliance with all applicable standards for ...
... Calif., March 6 Irvine Scientific, a leading medical ... to announce the addition of IS CHO-CD XP(TM) Culture ... CHO-CD XP(TM) Culture System is a set of chemically-defined*, ... technology to deliver robust cell growth and production for ...
Cached Biology Technology:Finesse Demonstrates TruFluor(TM) pH, a Single-Use Optical pH Sensor and Transmitter 2Cell Therapeutics Announces Receipt of NASDAQ Listing Update 2Cell Therapeutics Announces Receipt of NASDAQ Listing Update 3Irvine Scientific Launches IS CHO-CD XP(TM) 2
... Designed specifically for high efficiency ... Low toxicity reagent proven to ... range of cell types. Results ... 95% of target gene expression. ...
... a wide range of hands-on techniques that ... in all areas of protein chemistry and ... preface illustrates just how valuable this book ... chapters introduce the theoretical background of a ...
... easy-to-use siRNA transfection reagent that has been ... a variety of cell lines. jetSI-ENDO is ... straightforward protocol. High levels of gene silencing ... of 20 to 100 nM of siRNA ...
... reduced space requirements of the MassARRAY ... analysis technology affordable .The benchtop system ... SEQUENOM's applications and assays, including: ,, ... Gene Expression Analysis (QGE) , Quantitative ...
Biology Products: