Navigation Links
Hunt for human genes involved in cell division under way

A systematic search through human genes has begun at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. Working within the MitoCheck consortium that includes 10 other institutes throughout Europe, the EMBL scientists will silence all human genes, one-by-one, to find those involved in cell division (mitosis) and to answer fundamental questions of how cell division is regulated.

The scientists will use a method called 'RNA interference (RNAi)' where chemically synthesized RNA molecules are used to target and silence each human gene. About 22,000 genes will be suppressed and their impact on cell division monitored by live cell microscopy to understand each gene's role in cell division.

"To our knowledge, we are the first group to take on this systematic search through the genome in live cells. We will use the most potent RNAi reagent for this study, which is usually out of reach for academic labs because of the enormous cost and the ever-changing annotation of the human genome. But being part of the large EU project MitoCheck allowed us to work with one of the leading suppliers of siRNAs, Ambion Europe, Ltd., to produce a genome-wide library for this project," says Dr. Jan Ellenberg, EMBL Group Leader and co-initiator of the MitoCheck project.

Undertaking such a large project required tens of thousands of siRNA molecules, as two to three molecules are targeted specifically against each human gene. EMBL chose Ambion as a supplier of the siRNA library because its library could be tailored to the most up-to-date list of human target genes ?a list provided by the Wellcome Trust Sanger Institute, the MitoCheck bioinformatics partner. Also, Ambion's siRNA libraries utilize a siRNA design algorithm developed by Cenix BioScience GmbH (an EMBL spin-off company), leading to high efficacy of the siRNAs.

"We are happy to work with Ambion and Cenix BioScience on this ambitious project. This represents the first use of a genome-wi de library of chemically synthesized siRNAs in academic research and these partners have provided us with the tools to carry out this search," says Ellenberg. "Thousands of genes have been tested in the initial phase of the project and the results are very promising."

The systematic search and analysis of the screen is set to be completed by the end of 2005.


'"/>

Source:European Molecular Biology Laboratory


Related biology news :

1. Novel technology detects human DNA mutations
2. Current human embryonic stem cell lines contaminated UCSD/Salk team finds
3. UWs Rosetta software to unlock secrets of many human proteins
4. Genome-wide mouse study yields link to human leukemia
5. Found: Missing sequence of the human Y chromosome
6. Study finds more than one-third of human genome regulated by RNA
7. Molecular machine may lead to new drugs to combat human diseases
8. Sea skate experiment sheds light on human cell transport
9. Mouse brain tumors mimic those in human genetic disorder
10. Zebrafish may hold key to understanding human nerve cell development
11. Yale researchers identify molecule for detecting parasitic infection in humans
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... Florida , April 11, 2017 ... a security technology company, announces the appointment of independent Directors ... Bendheim to its Board of Directors, furthering the company,s ... ... of NXT-ID, we look forward to their guidance and benefiting ...
(Date:4/5/2017)... , April 5, 2017 Today ... announcing that the server component of the HYPR platform ... for providing the end-to-end security architecture that empowers biometric ... HYPR has already secured over 15 million users ... including manufacturers of connected home product suites and physical ...
(Date:4/3/2017)...  Data captured by IsoCode, IsoPlexis Corporation,s ... statistically significant association between the potency of ... objective response of cancer patients post-treatment. The ... cancer patients will respond to CAR-T cell ... to improve both pre-infusion potency testing and cell ...
Breaking Biology News(10 mins):
(Date:7/26/2017)... , ... July 26, 2017 , ... Vincent R. Burton, ... which is licensed by the Bahamas’ National Stem Cell Ethics Committee (NSCEC). , ... University of the West Indies. He went on to complete residency training in the ...
(Date:7/26/2017)... July 26th, 2017 /PRNewswire/ - Caprion Biosciences Inc., a world leading ... host blood proteins predictive of early stage (latent) Mycobacterium tuberculosis ... multiple reaction monitoring (MRM-MS) platform. ... Caprion Biosciences Inc. (CNW ... The new data, ...
(Date:7/26/2017)... ... July 25, 2017 , ... The leading Regenerative Veterinary Medicine ... cell therapy has been used on more than 13,000 animals. VetStem was ... fatal injuries to tendons and ligaments. , In 2003 VetStem signed a worldwide ...
(Date:7/26/2017)... ... 26, 2017 , ... Spectral imaging is a non-destructive technique ... This unique capability combines high resolution imaging with spectral analysis. The system ... for chemical analysis, quality control, and decision-making. , Snapshot spectral imaging is key ...
Breaking Biology Technology: