Behrouz Aflatoonian will tell the 21st annual conference of the European Society of Human Reproduction and Embryology today (Monday 20 June) that the research also solves the practical and ethical problems associated with obtaining human samples of primordial germ cells (PGCs), which are the ancestral cells that eventually form eggs and sperm (gametes). "Investigating the mechanisms of human primordial germ cell and gamete development is important for understanding the causes of infertility and the potential harmful effects of environmental chemicals on reproductive development," he will say. "But at present it is very difficult to obtain human samples of these cells as they only occur early in development."
Mr Aflatoonian, who is a PhD student in Professor Harry Moore's laboratory at the Centre for Stem Cell Biology, University of Sheffield, UK, said that studies with mice embryonic stem cells had shown that they were capable of differentiating into PGCs and subsequently eggs and sperm, so he set out to see if the same applied to human embryonic stem cells (HESCs).
"We derived six embryonic stem cell lines from embryos donated for research under HFEA regulations by couples undergoing IVF treatment. In addition, we utilised cell lines from the University of Wisconsin.
"The human embryonic stem cells were allowed to develop into collections of cells called embryoid bodies. The embryoid bodies were tested to see which genes were active, or 'expressed', in them and it was found that within two weeks a v
Source:European Society for Human Reproduction and Embryology