Navigation Links
Hopkins scientists uncover 'tags' that force proteins to cell surface

Discovery likely to streamline drug and vaccine development

Johns Hopkins scientists have discovered internal "shipping labels" that allow -- and perhaps force -- hundreds if not thousands of proteins to get to the surface of cells and stay there. Two natural proteins that use one of these "tags" are the ion channel that lets heart cells contract on cue, and the docking point that allows HIV, the virus that causes AIDS, into cells, the researchers discovered.

Because proteins on the cell surface are "lock-on" sites for drugs and other molecules, as well as triggers of immune reactions, the findings, described in the Sept. 11 advance online publications of Nature Cell Biology, might revolutionize efforts in drug and vaccine development, says the Hopkins team.

"A typical step in drug development is to get cells in a dish to express the protein you want to target with drugs, and then to test thousands of molecules to see which ones interact with the protein and have the effect you want," says the study's senior author, Min Li, Ph.D., a professor of neuroscience at the High Throughput Biology Center of the Johns Hopkins' Institute for Basic Biomedical Sciences.

"But if you can't get the protein to the cell surface, you can't use this screening technique. If we can force proteins to the cell surface, we can overcome obstacles that have prevented laboratory study of some really important proteins," says Li. The application of these surface tags to force protein transportation to the cell surface is the subject of a Patent Cooperation Treaty (PCT) patent application submitted by The Johns Hopkins University.

From among 25 billion randomly created, eight-building-block-long protein bits, postdoctoral fellow Sojin Shikano uncovered 65 that forced a normal protein to leave the cell's protein-building factory and go to the cell surface. By searching sequences of known human proteins, the researchers then identified those that use variatio
'"/>

Source:Johns Hopkins Medical Institutions


Page: 1 2 3 4

Related biology news :

1. Hopkins AIDS experts issue warning about global efforts to provide drug therapies
2. Johns Hopkins flu expert calls for mandatory vaccination of health care workers
3. Whole-genome study at Johns Hopkins reveals a new gene associated with abnormal heart rhythm
4. Hopkins researchers discover genetic switch that turns off an oxygen-poor cells combustion engine
5. Hopkins study suggests commercially available antibiotic may help fight dementia in HIV patients
6. Johns Hopkins lab scientists tame overactive CF protein
7. Hopkins researchers develop new tool to watch real-time chemical activity in cells
8. Hopkins scientists show hallucinogen in mushrooms creates universal mystical experience
9. Hopkins researchers discover how brain protein might control memory
10. Hopkins scientists link immune response to ghost parasites and severely congested sinuses
11. Wisconsin scientists grow critical nerve cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Hopkins scientists uncover tags that force proteins cell surface

(Date:9/1/2014)... increasing amount of research shows an association between TV ... lifestyle. Now, a new Cornell University study points out ... might lead people to eat twice as much as ... action movie while snacking your mouth will see more ... the new article just published in the Journal ...
(Date:9/1/2014)... quality in the U.S. has improved steadily in recent ... overall dietary quality remains poor and disparities continue to ... new study from Harvard School of Public Health (HSPH). ... date that the extensive efforts by many groups and ... payoff, but it also indicates that these efforts need ...
(Date:9/1/2014)... the U.S. diet showed some modest improvement in the ... in the consumption of unhealthy trans fats, but the ... and the poor. , , An unhealthy diet is ... Eating a healthy diet is an important part of ... trends in diet quality is important because it can ...
Breaking Biology News(10 mins):Can action movies make you fat? 2Quality of US diet shows modest improvement, but overall remains poor 2Quality of US diet shows modest improvement, but overall remains poor 3Quality of US diet improves, gap widens for quality between rich and poor 2Quality of US diet improves, gap widens for quality between rich and poor 3
... Biological Sciences Research Council (BBSRC)-led Integrated Biorefining Research and ... which could be used to make biofuel production more ... Issue of the American Chemical Society journal Biochemistry ... the Universities of Warwick and British Columbia, could make ...
... Carolina State University have developed a new technique ... a computer,s ability to build computer models of ... algorithms used to build models of biological systems ... can account for uncertainty and biological variation. This ...
... Institute has won a four-year, $1.9 million grant from ... parasite that causes malaria, laying the groundwork to develop ... "Many antimalarial drugs alleviate symptoms, but do ... malaria parasites are able to persist asymptomatically in the ...
Cached Biology News:First wood-digesting enzyme found in bacteria could boost biofuel production 2New parallelization technique boosts our ability to model biological systems 2Scripps Research scientist wins $1.9 million grant to study malaria 2
(Date:8/28/2014)... executives from clinical trial marketing firm BBK Worldwide will lead ... Clinical Trials 2014 , to be held at the Hyatt ... Patient recruitment experts Bonnie A. Brescia , Matthew ... on the benefits of employing an effective patient engagement strategy, ... mobile apps – can be used to drive study participation ...
(Date:8/28/2014)... WA (PRWEB) August 28, 2014 Sterlitech ... Filtration’s polymeric membranes to its stable of products ... available molecular weight cut-offs and include membrane filters with ... steady increase in customer demand for our membrane process ... greater variety of flat sheet membranes,” explains Sterlitech President ...
(Date:8/28/2014)... Exciting new work by a Florida State University ... that can take your temperature, emit white light, and ... the molecule looks like a butterfly. , Biwu Ma, ... Engineering in the FAMU-FSU College of Engineering, created the ... has continued to discover that his creation has many ...
(Date:8/28/2014)... August 28, 2014 A new ... the world is available from Strategic Consulting, Inc., ... “ Industrial Microbiology Market Review, Fourth Edition: Global ... ” (IMMR—4) tracks and compares microbiology test volumes, ... America, Europe and Asia, and forecasts future volumes ...
Breaking Biology Technology:BBK Worldwide Leads Sessions at Key September Events 2Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3
... , In this ... merits. A comprehensive examination of four Information Dependent Acquisition (IDA) survey ... found. A significant number of metabolites were found with each mode ... from glyburide. , Overview ...
... Overview , ... using isotope coded affinity tags is to correctly identify and ... spectrum generated by mass spectrometry. Because MALDI-TOF mass spectrometry can ... can be used to quickly screen the ICAT reagent pairs ...
... Jason S. Wood, Varian, Inc. , ... Cytochrome P450s (CYPs) are heme-containing enzymes ... . CYPs are part of the Phase I (oxidative) metabolism within ... enabling either easy elimination of the drug or further conjugation by ...
Cached Biology Technology:Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System 2Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System 3Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System 4Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System 5Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System 6Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool 2Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool 3Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method 2Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method 3
... rapid and efficient removal of short primers, ... from PCR products (Figure 1). The simple ... Buffer options for fragments >100 bp or ... either single column or 96-well plate format ...
Mus musculus junctophilin 2 (Jph2) Antigen: Recombinant Protein...
... Cloning Kits for Sequencing allow 5-minute cloning and ... TOPO Cloning vectors with primer sites located as ... product insertion site. This minimizes the amount of ... reaching the sequence of your insert.vectors are available. ...
2.0 GHz Compaq Computer with 512 MB RAM, 32 MB AGP Graphics Card, 40 GB Hard Drive, 1 Serial Port & Open PCI Slot, Windows 2000 OS, MS Office, CD Read/Writer and 18 Flat Panel Monitor...
Biology Products: