Navigation Links
High-throughput oncogene mutation detection in human cancers by mass spectrometry-based genotyping

of the tumor is conditioned around such mutations, so if you eliminate the oncogene, the tumor shrinks," Dr. Garraway said. Examples include the c-kit tyrosine kinase that drives development of gastrointestinal stromal tumors (GISTs), and certain EGFR mutations that are responsible for one form of lung cancer. These cancers can be treated with the targeted therapies Gleevec and Tarceva, respectively, which shut down the affected oncogenes.

The test involves taking a sample of fresh or frozen tumor, amplifying its DNA, and then searching for 250 known mutations in 17 oncogenes. (These include members of the ras, EGFR, pi3 and c-kit kinase families, among others.) To check performance of the genotyping, the research team tested over 1,000 tumors, representing 15 different cancer types, and then independently verified over half of the mutations they identified using traditional DNA sequencing or other methods. They found that over 92 percent of mutations identified by the mass spectrometry method were validated in this manner, and believe that much of the difference is due not to the mass spectrometry method but to limitations of the laborious sequencing test "which is not always sensitive in tumor specimens," Dr. Garraway said.

He added that the mass spectrometry test offers information that is distinct from the cancer microarray gene expression tests now being developed. "Those tests depend on gene expression while this one looks directly at problems in the genome," Dr. Garraway said.


'"/>

Source:American Association for Cancer Research


Page: 1 2

Related biology news :

1. Scientists tie several cancers to common oncogene engine
2. Pair of microRNA molecules controls major oncogene in most common leukemia
3. Novel technology detects human DNA mutations
4. Fox Chase Cancer Center scientists identify immune-system mutation
5. Mitochondrial DNA mutations play significant role in prostate cancer
6. Same mutation aided evolution in many fish species, Stanford study finds
7. Bioelectronic ‘signatures?hold key to DNA mutations
8. Sugar beet virus mutation requires Texas touch
9. Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations
10. Treatments have same target, different responses for lung cancer patients with genetic mutation
11. Newly recognized gene mutation may reduce seeds, resurrect plants
Post Your Comments:
(Date:5/24/2013)... by avoiding detection and destruction by the immune system. ... cancer cells; however, there are many factors that cause ... cells are immune cells that function to suppress the ... Journal of Clinical Investigation , researchers led by Ronald ... that infiltrate tumors express proteins that can be targeted ...
(Date:5/23/2013)... , Automated conserved noncoding sequence (CNS) discovery reveals ... , Within the genome of each species, there ... change in position and sequence over millions of years, ... these evolutionarily stable sequences, so-called conserved noncoding sequences (CNSs), ... or the condensation of chromosomes, but the function of ...
(Date:5/23/2013)... mechanism by which estrogen suppresses lipid synthesis in the ... approach toward treating certain liver diseases. , With ... are changing long-held views in the field. Study results ... Science Signaling . , "The dogma in the ... only receptors located in the nucleus respond to steroid ...
Breaking Biology News(10 mins):Frontiers news briefs: May 23 2Frontiers news briefs: May 23 3UCI study reveals new mechanism for estrogen suppression of liver lipid synthesis 2
... 2008) -- Scientists and researchers have taken a big ... strain of avian influenza, or "bird flu," the potential ... infected nearly 400 people in 14 countries since it ... Department of Energy,s (DOE) Argonne National Laboratory, in conjunction ...
... last 10 years has seen a huge increase in the ... being kept in our homes are monkeys, tarantulas, iguanas, salamanders, ... reserves around the world develop their conservation and captive breeding ... husbandry of exotic animals. To help develop ...
... 2008 A systematic literature review conducted by a ... Hispanic Health Council (Hartford), and the Connecticut Center for ... peer education/counseling on nutrition and health outcomes among Latinos ... the July/August issue of the Journal of Nutrition ...
Cached Biology News:Newly described 'dragon' protein could be key to bird flu cure 2The exotic side of veterinary science 2The exotic side of veterinary science 3Peers important for nutrition education among Latinos 2
(Date:5/23/2013)... The New Jersey chapter of the largest ... Associates (BDPA), is hosting its 10th annual Families in ... event will encourage students and their parents to explore ... from many of the industries’ finest teachers. Held from ... Institute of Technology, the event will include workshops, instruction ...
(Date:5/23/2013)... 23, 2013 During the conduct ... to adhere to principles of good clinical practice ... speaker Dr. Jeremy Lim, Fellow, Clinical Development at ... ,     Understand the International Conference on Harmonization ... additional regulations for the conduction of clinical trials ...
(Date:5/23/2013)... (PRWEB) May 23, 2013 ... ancestry tracing for people of African descent, today ... product offering. For people of all races, myDNAmix ... from five distinct populations: Indigenous Americas (Native American), ... India Subcontinent. , Originally launched in ...
(Date:5/23/2013)... (PRWEB) May 23, 2013 Watlow®, ... of complete thermal systems, recently introduced its new ... years of experience in designing and manufacturing industrial ... of products to serve diverse customer needs. , ... featured at the beginning of the book. These ...
Breaking Biology Technology:Black Data Processing Associates (BDPA) New Jersey Hosts 10th Annual Families in Technology Day June 8, 2013 2Adhering to Good Clinical Practice, New Webinar Hosted by Xtalks 2AfricanAncestry.com Rolls Out Improved MyDNAMix Admixture Test for People of all Races; Enhancements Increase Accuracy in Estimation of Ancestral Makeup 2AfricanAncestry.com Rolls Out Improved MyDNAMix Admixture Test for People of all Races; Enhancements Increase Accuracy in Estimation of Ancestral Makeup 3Watlow Introduces Its New Industrial Heater Catalog 2
... to convert metallic wastes into high-value catalysts for generating ... of Microbiology . Researchers from the School ... the mechanisms that allow the common soil bacterium ... from industrial waste sources. Palladium is one of ...
... the National Institute of Standards and Technology (NIST) have ... measurementscritical data for a wide variety of fields dealing ... small as a few nanoliters. Currently a table-top prototype, ... for biotechnologists studying minute quantities of complex materials that ...
... Ont. (Wednesday, Sept. 1, 2010) - A new ... - developed by University of Waterloo engineering researchers ... such as medical devices, microelectromechanical systems (MEMS), printers, ... The breakthrough technology will provide engineers with ...
Cached Biology Technology:Metal-mining bacteria are green chemists 2Micro rheometer is latest Lab On a Chip device 2New process promises to revolutionize manufacturing of products 2
... Protein determined by Biuret. Physical ... (NH 4 ) 2 SO ... Note: Affinity purified • Physical ... Additives: MgCl 2 1 ...
... MW: 60kDa (A) and 36kDa (C) ... pH 7.5, 500mM NaCl, 60mM 2-mercaptoethanol, ... 1mM DTT, and 0.1mg/ml serum albumin ... of proteins. Purification: DEAE chromatography, aminohexyl-agarose ...
... IT siRNA Tracker Intracellular Localization Kit provides ... and deliver siRNA, of your design, in ... vitro tracking experiments. Subcellular localization and functional ... monitored following introduction of the labeled siRNA ...
Selective media for Salmonella...
Biology Products: