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Helping chlorine-eating bacteria clean up toxic waste

nes strains under a variety of different conditions, such as exposing them to different chlorinated compounds one at a time, varying the environment or the nutrients supplied, and then observing which genes are expressed and what proteins are manufactured. The data will go to GNS, which will try to build computer models of how the bacteria's proteins work together under each condition and whether the pathway for each condition is the same for PCE and TCE, and if not, what steps they have in common.

It will be an "iterative process," Richardson said. If a model shows that changing a particular condition produces a particular result, the lab will try it out and see if the result matches the model. Eventually, Richardson said, some commonalities should appear.

"There will be a suite of models, and we can highlight features that are common across several models," she said. "As we develop the model, we can begin to look at the genomes of other strains of Dehalococcoides. If genes that are important in our strain are found in others ... then we can do the same experiments with the others." Finding which genes are at work with which pollutants might lead to understanding how to remediate other kinds of pollutants, such as PCBs, dioxins, chlorobenzenes or chlorophenols.

Richardson, who grew up in the Hudson River Valley, notes that such pollutants are common in the river's harbors. "There are still thousands of sites around the country that need to be cleaned up," she said. "Ithaca has three or four, and that's not atypical."
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Source:Cornell University News Service


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