Navigation Links
Health costs soar as 60 million Americans classed as obese

A new method for manipulating macromolecules has been developed by researchers at the University of Illinois at Urbana-Champaign. The technique uses double-stranded DNA to direct the behavior of other molecules.

In previous DNA nanotechnology efforts, duplex DNA has been used as a static lattice to construct geometrical objects in three dimensions. Instead of manipulating DNA alone into such shapes, the researchers are using DNA to control the folding and resulting structure of RNA. Eventually, they envision building supramolecular machines whose inner workings are governed by twisted strands of DNA.

In a paper that has been accepted for publication in the Journal of the American Chemical Society, and posted on its Web site, Silverman and graduate student Chandrasekhar Miduturu begin with a piece of unfolded RNA. Through specific chemical reactions, they attach two strands of DNA, each resembling one side of a ladder. The two DNA strands spontaneously bind together, then the researchers add magnesium ions to initiate folding of the RNA.

"Folding of the RNA structure competes with formation of the DNA constraint until a chemical balance is reached," Silverman said. "In some cases, the DNA is like a barnacle, just stuck onto the RNA without perturbing its structure. In other cases, the DNA changes the RNA structure. We can predict which situation will occur based on the shape of the RNA and on the attachment points of the DNA constraint."

In cases where the normal RNA shape and the DNA constraint cannot co-exist simultaneously, the balance between competing RNA and DNA structures is controlled by the concentration of magnesium ions, Silverman said.

In work not yet published, the researchers have also shown that the effects of the DNA constraint on the RNA structure can be modulated by external stimuli such as DNA oligonucleotide strands, protein enzymes and chemical reagents.

While Silverman and Miduturu are currently usi ng RNA as a proof of principle for their DNA constraint studies, they also plan to use the new technique to more effectively study the folding process of RNA. Because they can control RNA structure precisely, they could generate and examine biologically relevant folded and misfolded RNAs. They could also hook the DNA constraints to other molecules, including non-biological macromolecules, to control their folding.

Importantly, the process of manipulating macromolecules with DNA constraints can be either reversible or irreversible, depending on which chemical trigger is used. Like a switch, a particular molecular shape could be turned on and off.

"Another key aspect of DNA constraints is their programmability," Silverman said. "By placing two or more constraints on one molecule, we could generate multiple molecular states that would be programmable by DNA sequence. In other efforts, we would like to control macroscopic assembly processes by influencing the shapes of self-assembling molecular components."


'"/>

Source:


Related biology news :

1. BioMed Central welcomes the new National Institutes of Health public access policy
2. OneWorld Health drug receives Orphan designation from U.S. and European regulatory agencies
3. Going To Extremes To Improve Human Health
4. Patient-choice C-section rate rises 36%: HealthGrades study
5. Grand Challenges in Global Health initiative funds Yale project
6. Health benefits of a Christmas brandy
7. Health of coral reefs detected from orbit
8. Health of Acehnese reefs in the wake of the tsunami shows human impacts more harmful
9. Healthy coral reefs of Madagascar resisting damage from climate change
10. Health Canada approves cold and flu medicine
11. Mailman School of Public Health researchers report blood DNA can be early predictor of liver cancer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/20/2016)... , June 20, 2016 Securus ... justice technology solutions for public safety, investigation, corrections ... the prisons involved, it has secured the final ... (DOC) facilities for Managed Access Systems (MAS) installed. ... additional facilities to be installed by October, 2016. ...
(Date:6/7/2016)... June 7, 2016  Syngrafii Inc. and San ... relationship that includes integrating Syngrafii,s patented LongPen™ eSignature ... This collaboration will result in greater convenience for ... union, while maintaining existing document workflow and compliance ... ...
(Date:6/1/2016)... , June 1, 2016 Favorable ... Election Administration and Criminal Identification to Boost Global Biometrics ... recently released TechSci Research report, " Global Biometrics Market ... Competition Forecast and Opportunities, 2011 - 2021", the global ... by 2021, on account of growing security concerns across ...
Breaking Biology News(10 mins):
(Date:6/23/2016)...   EpiBiome , a precision microbiome engineering company, ... financing from Silicon Valley Bank (SVB). The financing will ... its drug development efforts, as well as purchase additional ... has been an incredible strategic partner to us – ... would provide," said Dr. Aeron Tynes Hammack , ...
(Date:6/23/2016)... LOUISVILLE, Ky. , June 23, 2016 /PRNewswire/ ... from two Phase 1 clinical trials of its ... double-blind, placebo-controlled, single and multiple ascending dose studies ... and pharmacodynamics (PD) of subcutaneous injection in healthy ... APL-2 subcutaneously (SC) either as a single dose ...
(Date:6/23/2016)... ... 23, 2016 , ... ClinCapture, the only free validated electronic ... showcase its product’s latest features from June 26 to June 30, 2016 for ... Disrupting Clinical Trials in The Cloud during the conference. DIA (Drug Information ...
(Date:6/23/2016)... , June 23, 2016 ... research report to its pharmaceuticals section with historic ... details and much more. Complete report ... 151 pages, profiling 15 companies and supported with ... http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . The Global ...
Breaking Biology Technology: