Navigation Links
Health costs soar as 60 million Americans classed as obese

A new method for manipulating macromolecules has been developed by researchers at the University of Illinois at Urbana-Champaign. The technique uses double-stranded DNA to direct the behavior of other molecules.

In previous DNA nanotechnology efforts, duplex DNA has been used as a static lattice to construct geometrical objects in three dimensions. Instead of manipulating DNA alone into such shapes, the researchers are using DNA to control the folding and resulting structure of RNA. Eventually, they envision building supramolecular machines whose inner workings are governed by twisted strands of DNA.

In a paper that has been accepted for publication in the Journal of the American Chemical Society, and posted on its Web site, Silverman and graduate student Chandrasekhar Miduturu begin with a piece of unfolded RNA. Through specific chemical reactions, they attach two strands of DNA, each resembling one side of a ladder. The two DNA strands spontaneously bind together, then the researchers add magnesium ions to initiate folding of the RNA.

"Folding of the RNA structure competes with formation of the DNA constraint until a chemical balance is reached," Silverman said. "In some cases, the DNA is like a barnacle, just stuck onto the RNA without perturbing its structure. In other cases, the DNA changes the RNA structure. We can predict which situation will occur based on the shape of the RNA and on the attachment points of the DNA constraint."

In cases where the normal RNA shape and the DNA constraint cannot co-exist simultaneously, the balance between competing RNA and DNA structures is controlled by the concentration of magnesium ions, Silverman said.

In work not yet published, the researchers have also shown that the effects of the DNA constraint on the RNA structure can be modulated by external stimuli such as DNA oligonucleotide strands, protein enzymes and chemical reagents.

While Silverman and Miduturu are currently usi ng RNA as a proof of principle for their DNA constraint studies, they also plan to use the new technique to more effectively study the folding process of RNA. Because they can control RNA structure precisely, they could generate and examine biologically relevant folded and misfolded RNAs. They could also hook the DNA constraints to other molecules, including non-biological macromolecules, to control their folding.

Importantly, the process of manipulating macromolecules with DNA constraints can be either reversible or irreversible, depending on which chemical trigger is used. Like a switch, a particular molecular shape could be turned on and off.

"Another key aspect of DNA constraints is their programmability," Silverman said. "By placing two or more constraints on one molecule, we could generate multiple molecular states that would be programmable by DNA sequence. In other efforts, we would like to control macroscopic assembly processes by influencing the shapes of self-assembling molecular components."


'"/>

Source:


Related biology news :

1. BioMed Central welcomes the new National Institutes of Health public access policy
2. OneWorld Health drug receives Orphan designation from U.S. and European regulatory agencies
3. Going To Extremes To Improve Human Health
4. Patient-choice C-section rate rises 36%: HealthGrades study
5. Grand Challenges in Global Health initiative funds Yale project
6. Health benefits of a Christmas brandy
7. Health of coral reefs detected from orbit
8. Health of Acehnese reefs in the wake of the tsunami shows human impacts more harmful
9. Healthy coral reefs of Madagascar resisting damage from climate change
10. Health Canada approves cold and flu medicine
11. Mailman School of Public Health researchers report blood DNA can be early predictor of liver cancer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/13/2017)... PUNE, India , April 13, 2017 According ... Identity Proofing, Identity Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication ... by MarketsandMarkets™, the IAM Market is expected to grow from USD 14.30 ... Annual Growth Rate (CAGR) of 17.3%. ... ...
(Date:4/11/2017)... , April 11, 2017 NXT-ID, Inc. ... technology company, announces the appointment of independent Directors Mr. ... to its Board of Directors, furthering the company,s corporate governance ... Gino ... we look forward to their guidance and benefiting from their ...
(Date:4/5/2017)... KEY FINDINGS The global market ... CAGR of 25.76% during the forecast period of 2017-2025. ... for the growth of the stem cell market. ... MARKET INSIGHTS The global stem cell market is segmented ... The stem cell market of the product is segmented ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... , ... October 12, 2017 , ... ... with the pharmaceutical and biotechnology industries to improve patient outcomes and quality of ... trends in analytical testing are being attributed to new regulatory requirements for all ...
(Date:10/11/2017)... NJ (PRWEB) , ... October 11, 2017 , ... Personal eye wash is a basic ... one eye at a time. So which eye do you rinse first if a dangerous ... have Plum Duo Eye Wash with its unique dual eye piece. , “Whether ...
(Date:10/11/2017)... LAGUNA HILLS, Calif. , Oct. 11, 2017  SkylineDx ... London (ICR) and University of Leeds ... to risk-stratify patients with multiple myeloma (MM), in a multi-centric ... The University of Leeds is the ... UK, and ICR will perform the testing services to include ...
(Date:10/10/2017)... ... 10, 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, ... The bold new look is part of a transformation to increase awareness, appeal ... growth period. , It will also expand its service offering from its signature gourmet ...
Breaking Biology Technology: