Navigation Links
Growing nerve cells in 3-D dramatically affects gene expression

When it comes to growing cells in a lab, technique matters. A new Brown University study shows that nerve cells grown in three-dimensional cultures use 1,766 genes differently compared to nerve cells grown in standard two-dimensional petri dishes.

The study, published in the May issue of Tissue Engineering, adds to a growing body of research showing that culture techniques can significantly affect cell growth and function. This research shows that cells grown in a laboratory in 3-D environments, not in flat petri dishes, are more like cells grown in the ultimate 3-D environment ?the human body.

"More and more, we're seeing evidence that cells cultured in three dimensions look and behave more like cells in your body," said Diane Hoffman-Kim, the Brown bioengineer who spearheaded the new study, "so culture method is critical. If you want to better understand how the human body behaves or how new drugs might fight disease, 3-D may be a better bet."

For more than 100 years, scientists have grown human cells in flat dishes. In these 2-D glass incubators, better known as petri dishes, cells stick to the bottom and spread out as they multiply. But in the body, cells don't grow that way. They are suspended in fluids and gels and surrounded by other cells. And these cells aren't stuck; they move.

As a result, some scientists suspect that hothouse cells do not behave like in vivo varieties. This means that the critical functions scientists are trying to understand by studying these cells ?from the proliferation of cancer to the bacterial assault by antibiotics ?may play out differently. Studies indeed show differences in behavior between cells cultured in 2-D and in 3-D. Cells cultured in 3-D, for example, grow faster.

Hoffman-Kim, an assistant professor of medical science and engineering at Brown, wants to find a way to repair damaged nerve tissue. To get there, she needs to grow nerve cells that will flourish when plac
'"/>

Source:Brown University


Page: 1 2

Related biology news :

1. Growing crops to cope with climate change
2. Wisconsin scientists grow critical nerve cells
3. Clam embryo study shows pollutant mixture adversely affects nerve cell development
4. Zebrafish may hold key to understanding human nerve cell development
5. New component of the brakes on nerve regeneration found
6. Cerebral navigation: How do nerve fibers know what direction to grow in?
7. Molecular messengers perform a crucial role in the ability of injured nerve cells to heal themselves
8. Diabetic nerve therapy shows striking results
9. Malfunctioning bone marrow cells sabotage nerve cells in diabetes
10. UIC researchers show protein routes messages in nerve cells
11. Researchers find molecule that inhibits regrowth of spinal nerve cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Growing nerve cells dramatically affects gene expression

(Date:5/16/2013)... FASEB MARC (Maximizing Access to Research Careers) Program ... Society for Developmental Biology (SDB) 72nd Annual Meeting ... awards are meant to promote the entry of ... the mainstream of the basic science community and ... the SDB 2013 Annual Meeting. , Awards are ...
(Date:5/16/2013)... Northwestern University scientists have shown a gene involved in ... proper function of the circadian clock. , In ... found the gene, called Ataxin-2, keeps the clock responsible ... the gene, the rhythm of the fruit fly,s sleep-wake ... schedule difficult for the fly. , The discovery ...
(Date:5/16/2013)... 2013 The relationship between the heritable risk ... been clear. Schizophrenia is commonly associated with cognitive ... clues that reduced IQ may be linked to ... cognitive ability may precede the onset of schizophrenia ... healthy relatives of people diagnosed with schizophrenia. , ...
Breaking Biology News(10 mins):Gene involved in neurodegeneration keeps clock running 2Genetic risk for schizophrenia is connected to reduced IQ 2
... rich in natural antioxidants improves insulin sensitivity in insulin-resistant ... drug metformin, a preliminary study from Italy finds. The ... 92nd Annual Meeting in San Diego. "The beneficial ... for the first time one of their biological bases ...
... - A team of bioengineers from the University of ... computer neural network model to accurately predict how blood ... heart attack or stroke. Using an automated, robotic ... different combinations of biological stimuli like those experienced during ...
... five colleagues from other institutions have published in ... particularly in the Western Antarctic Peninsula, or WAP. ... toward South America. In their review paper, the co-authors, ... argue that research in this region is imperative because ...
Cached Biology News:Well-defined quantity of antioxidants in diet can improve insulin resistance 2Penn bioengineers create simulator to test blood platelets in virtual heart attacks 2Penn bioengineers create simulator to test blood platelets in virtual heart attacks 3Oceanographers call for more ocean observing in Antarctica 2
(Date:5/16/2013)... FL (PRWEB) May 16, 2013 ... named Barbara A. Myers, CAE, to the newly ... In her new role, Myers will be responsible ... development and production strategies, advancing the Society’s Member-led ... processes and continuing to refine organizational, membership and ...
(Date:5/16/2013)... Ill. (PRWEB) May 16, 2013 ... and portable models, from Cole-Parmer is designed to ... enhanced line includes: , The new Cole-Parmer® ... true workhorses in the lab. They ensure a ... and plasma specimen separation. Their standard four-place swinging ...
(Date:5/16/2013)... , May 16, 2013  HealthSparq, a trusted ... the nation,s largest regional health plans, will debut ... Insurance Plans (AHIP) Institute 2013 conference on June ... results showing how health insurance companies across the ... consumer and marketplace demands for increased healthcare transparency. ...
(Date:5/16/2013)... As the weather warms, women everywhere are waiting for ... most make a statement with fashion, many change their makeup ... weather mixes makeup and sweat to produce, and increase acne ... during warmer months, Adult Acne treatment , Probiotic Action ... ways to deter breakouts. , Recently, an article from ...
Breaking Biology Technology:ISPE Names Barbara A. Myers, CAE, as Vice President of Professional Development 2Cole-Parmer Offers Enhanced Selection of Centrifuges 2HealthSparq to Reveal New Healthcare Transparency Benchmark Study at AHIP Institute 2013 2HealthSparq to Reveal New Healthcare Transparency Benchmark Study at AHIP Institute 2013 3Adult Acne Treatment, Probiotic Action Shares New Tips for Women on How to Fight Acne While Wearing Makeup 2
... Mass., Jan. 31 Inverness Medical,Innovations, Inc. (Amex: ... of rapid,diagnostic products for the consumer and professional ... 2008 Merrill Lynch Global,Pharmaceutical, Biotechnology and Medical Device ... the Grand Hotel Hyatt in New York City. ...
... PALO ALTO, Calif., Jan. 31 CV Therapeutics,Inc. (Nasdaq: ... an Investor Science,Day for the financial community in New ... a.m. EST., The event will be webcast live ... http://www.cvt.com ., About CV Therapeutics, CV Therapeutics, ...
... Jan. 31 Scientists from,deCODE genetics (Nasdaq: DCGN ... in the sequence of the human genome (SNPs) that,regulate ... the two,SNPs, located on chromosome 4p16, have a genome-wide ... the genome that occurs in the formation,of eggs and ...
Cached Biology Technology:Inverness Medical Innovations to Participate at 2008 Merrill Lynch Global Pharmaceutical, Biotechnology and Medical Device Conference on February 5, 2008 2CV Therapeutics to Host Investor Science Day on Thursday, February 7, 2008 2deCODE Discovers Gene Variants that May Help to Distribute the Work of Evolution between Men and Women 2deCODE Discovers Gene Variants that May Help to Distribute the Work of Evolution between Men and Women 3deCODE Discovers Gene Variants that May Help to Distribute the Work of Evolution between Men and Women 4
Immunogen: Synthetic peptide: M(1) C V P R S G L I L S(11) Storage: -20 C, Avoid Freeze/Thaw Cycles...
... pPIC3.5K vectors carry the kanamycin resistance gene ... Pichia. Spontaneous generation of multiple insertion events ... levels of Geneticin Reagent. Pichia transformants are ... their level of resistance to Geneticin Reagent. ...
...
Liquid. Phycoerythrin conjugate is in PBS, pH 7.4 containing 1% BSA and 0.1% sodium azide....
Biology Products: