Navigation Links
Groovy protein essential for promoting cancer development

s for thinking about the problem," he said. "There was no picture in any detail of what any part of this protein looks like."

Many labs have been working toward developing that picture, but the task has proven challenging. That's because the enzyme tends to clump together once outside of cells, preventing it from forming the ordered crystals necessary for structural studies. Scientists from Cech's lab and others had tried to simplify matters by crystallizing a portion of the protein, but the segments that they selected clumped together just as stubbornly as the whole enzyme.

Jacobs, a Damon Runyon Cancer Research Foundation fellow in Cech's lab and the first author of the paper, developed a new approach. With the help of bacteria and a protein that emits green fluorescent light, Jacobs randomly screened tens of thousands of fragments of the enzyme for one that would lend itself to successful structural analysis. His strategy took advantage of the fact that when multiple copies of the fluorescent protein clump together, the fluorescence is quenched, or extinguished. So Jacobs engineered bacteria to produce fragments of the telomerase enzyme fused to the fluorescent protein. Since telomerase fragments that clustered together would drag along ?and quench ?their associated fluorescent protein, Jacobs knew that any bright green bacterial colonies were producing protein fragments that remained free. Those rare colonies would be the best candidates for further analysis.

Jacobs performed these experiments on fragments of the telomerase enzyme from a variety of organisms, and found that only a fragment from Tetrahymena -- the single-celled organism in which telomerase was first discovered -- would work. The researchers named the protein fragment "telomerase essential N-terminal" (TEN) domain, in reference to its position within the complete enzyme. It took a few more biochemical tricks, but eventually Jacobs crystallized the protein fragmen
'"/>

Source:Howard Hughes Medical Institute


Page: 1 2 3 4

Related biology news :

1. New, automated tool successfully classifies and relates proteins in unprecedented way
2. New binding target for oncogenic viral protein
3. Controversial drug shown to act on brain protein to cut alcohol use
4. Timing is everything: First step in protein building revealed
5. UWs Rosetta software to unlock secrets of many human proteins
6. Researchers find how protein allows insects to detect and respond to pheromones
7. Signaling protein builds bigger, better bones in mice
8. Ancient olfaction protein is shared by many bugs, offering new pest control target
9. Automatic extraction of gene/protein biological functions from biomedical text
10. Discovery of key proteins shape could lead to improved bacterial pneumonia vaccine
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Groovy protein essential for promoting cancer development

(Date:7/30/2014)... stem cells can develop into a multitude of cells types. ... into the specific types of mature cells that make up ... One key seems to be long chains of sugars that ... group at the University of California, San Diego, has created ... but can be more easily manipulated to direct the process, ...
(Date:7/30/2014)... July 30, 2014 The Nano-Bio Manufacturing ... States Air Force Research Laboratory (AFRL), has awarded ... Binghamton University to develop electronics and ... The $425,000 project, with contributions from the University ... Electronics, Inc. ( Endicott, N.Y. ), ...
(Date:7/30/2014)... a paper published online today in the journal ... researchers Evan Kodra and Auroop Ganguly found that while ... variability in temperature extremes. For instance, while each year,s ... averages will also tend to fall within a wider ... are currently being observed. This means that even as ...
Breaking Biology News(10 mins):Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 2Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 3Big data confirms climate extremes are here to stay 2
... in common with mustard except hotdogs. Yet based on ... scientists have discovered a possible explanation for how organisms, ... the growth of organs. These findings overturn conventional views ... light on the development of more productive plants and ...
... and engineers in Antarctica, funded by the National Science Foundation ... past climate and greenhouse gases in the atmosphere that extends ... Sheet (WAIS), the ice containing the record is known as ... make up the core contain uniquely detailed information on past ...
... from Indiana University and Brown University believes it has ... and nuclear DNA produce an incompatible genotype that reduces ... The new research, led by IU biologists Kristi ... researcher Mo Siddiq, describes the cause and consequences of ...
Cached Biology News:Plants cut the mustard for basic discoveries in metabolism 2Plants cut the mustard for basic discoveries in metabolism 3Antarctic ice core contains unrivaled detail of past climate 2Antarctic ice core contains unrivaled detail of past climate 3Biologists map rare case of fitness-reducing interaction in nuclear, mitochondrial DNA 2
(Date:7/30/2014)... SAN FRANCISCO , July 30, 2014 /PRNewswire/ ... Transplant Congress evaluated the safety and efficacy of ... antibody-mediated rejection following kidney transplants in highly sensitized ... important inhibitor of the complement system. ... results in significant increases in the levels of ...
(Date:7/30/2014)... 2014 While administrative claims and ... research for a relatively long period of time, ... the use of these ‘big data’ for comparisons ... combining today’s powerful computer technology with the plethora ... data’ holds significant promise for identifying optimal interventions ...
(Date:7/30/2014)... 30, 2014   Wyle has been ... Lifecycle Mission Support Services (FILMSS) contract awarded by ... the terms of the contract, Wyle will provide ... Field, Calif. In particular, Wyle will provide program ... projects on the International Space Station and collaborative ...
(Date:7/30/2014)... BCC Research ( http://www.bccresearch.com ) reveals in ... Industrial Applications , the global market for industrial enzymes ... 2018, registering a five-year compound annual growth rate (CAGR) ... in the detergent enzyme segment with a CAGR of ... influenced almost every sector of industrial activity, ranging from ...
Breaking Biology Technology:Study Suggests C1-INH May Aid in Prevention of Antibody-Mediated Rejection Following Kidney Transplant 2Study Suggests C1-INH May Aid in Prevention of Antibody-Mediated Rejection Following Kidney Transplant 3Leveraging ‘Big Data’ - Methodological Considerations in Health Services Research, New Webinar Hosted by Xtalks 2Wyle Wins NASA's Fully Integrated Lifecycle Mission Support Services Contract 2Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 2Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 3
... 2012 Sofie Biosciences, Inc. , offering a comprehensive ... PET molecular imaging probes to better understand human disease, has ... by the United States Patent Office on technology that has ... of novel PET probes, known as [18F]FAC. Positron ...
... VA -- This time of year parents in northern ... activities. In addition to layers of warm clothing, gloves, ... available to mitigate the potential hazards of winter sports ... protect children,s heads from the stresses and forces that ...
... as it turns out, largely transparent to water. ... Polytechnic Institute (RPI) has determined that gold, copper and silicon ... layer of graphene as they would without. The research, ... Materials , is significant for scientists learning to fine-tune surface ...
Cached Biology Technology:Sofie Biosciences Is Awarded a US Patent on a Family of Novel PET Probes for Use in Oncology and Immunology 2Sofie Biosciences Is Awarded a US Patent on a Family of Novel PET Probes for Use in Oncology and Immunology 3How well does protective headgear work for small children engaged in Winter activities? 2Water sees right through graphene 2
Mouse/Rat WNK1 Affinity Purified Polyclonal Ab...
... of excess dye terminators, template DNA, primers, ... true sequencing products have proved critical for ... is designed to work with dye terminator ... DYEnamicTM ET. The unique uniformity of the ...
Enodgenous alkaline phosphotase inhibitor. Levamisole solution is supplied in concentrated form (20x). The final concentration of levamisole in the substrate solution will be 1 mM....
uncoupling protein 2 (mitochondrial, proton carrier), mRNA (cDNA clone MGC:19567 IMAGE:4335185), complete cds...
Biology Products: