Navigation Links
Gold nanoparticles could improve antisense cancer drugs

In the fight against cancer, antisense drugs, which prevent genes from producing harmful proteins such as those that cause cancer, have the promise to be more effective than conventional drugs, but the pace of development of these new drugs has been slow.

Using gold nanoparticles combined with DNA, scientists at Northwestern University now have demonstrated a new method for developing antisense drugs that outperform conventional antisense agents. The findings will be published May 19 in the journal Science.

A major challenge has been delivering antisense drugs to cells inside the body while avoiding their break down along the way. The Northwestern team shows that by attaching multiple strands of antisense DNA to the surface of a gold nanoparticle (forming an "antisense nanoparticle") the DNA becomes more stable and can bind to the target messenger RNA (mRNA) more effectively than DNA that is not attached to a nanoparticle surface (as in commercial agents).

When compared to antisense DNA complexed with commercial agents such as Lipofectamine and Cytofectin, the antisense nanoparticles were more effective in gene knockdown (decreasing gene expression and protein production), were less susceptible to degradation resulting in longer lifetimes, exhibited lower toxicity and were more readily absorbed by cells, exhibiting a greater than 99 percent uptake.

"When mutations in the body's genetic material cause too many copies of certain proteins, cancer and other diseases can result," said Chad A. Mirkin, director of Northwestern's Center for Cancer Nanotechnology Excellence, who led the study. "Whereas typical drugs target the proteins, it is possible through gene therapy to target the genetic material itself before it is ever made into copies of harmful proteins. One way to target the genetic material is to block the messenger RNA by using 'antisense DNA,' which prevents the message from ever becoming a protein."

Once inside cells, the DNA-modified nanoparticles act as messenger RNA "sponges" that bind to their targets and prevent them from being converted into proteins.

In their experiments the researchers targeted mRNA sequences that code for enhanced green fluorescent protein (EGFP) expressed in a mouse cell. The antisense sequence of the DNA attached to the nanoparticles was complementary to the mRNA for EGFP expression. When the nanoparticles were introduced to the cells the fluorescence dimmed -- a result of the nanoparticles binding to the mRNA and shutting down the protein's expression, or fluorescence.

"In the future, this exciting new class of antisense material could be used for the treatment of cancer and other diseases that have a genetic basis," said Mirkin, who is George B. Rathmann Professor of Chemistry, professor of medicine and professor of materials science and engineering.

In addition to Mirkin, other authors on the Science paper are Nathaniel L. Rosi (co-first author), David A. Giljohann (co-first author), C. Shad Thaxton, Abigail K. R. Lytton-Jean and Min Su Han, all from Northwestern University.


'"/>

Source:Northwestern University


Related biology news :

1. Probing the promise and perils of nanoparticles
2. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma
3. Using nanoparticles, in vivo gene therapy activates brain stem cells
4. NJIT study shows nanoparticles could damage plant life
5. MIT nanoparticles may help detect, treat tumors
6. Quantum dots reviewed -- Could these nanoparticles hold the cure to cancer?
7. Electric jolt triggers release of biomolecules, nanoparticles
8. U-M researchers use nanoparticles to target brain cancer
9. Gold nanoparticles prove to be hot stuff
10. Widely used iron nanoparticles exhibit toxic effects on neuronal cells
11. Coated nanoparticles solve sticky drug-delivery problem
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/26/2018)... (PRWEB) , ... October 26, 2018 , ... ... Dr. Donnica Moore, MD, President of Sapphire Women’s Health Group and prominent ... scientists and doctors from around the world, Dr. Moore will bring to uBiome ...
(Date:10/25/2018)... ... October 24, 2018 , ... ... to accommodate the company’s continued growth and success. This expansion announcement comes on ... Alliance’s “Sharing Conference.” This was the seventh consecutive Sharing Conference for RxS, where ...
(Date:10/24/2018)... ... October 22, 2018 , ... Myoscience Announces Commercial Release ... display at 2018 Annual Assembly of AAPM&R Society in Orlando , FREMONT, CA ... Nerve Stim Enabled Smart Tip 1x90mm, the latest innovative addition to Myoscience’s iovera° ...
Breaking Biology News(10 mins):
(Date:11/3/2018)... , ... November 01, 2018 , ... Triangle Insights Group, ... Magazine as one of the fastest-growing private companies in the United States, appearing on ... list ranks private companies based on percentage revenue growth over the previous three years. ...
(Date:10/31/2018)... ... 2018 , ... Pippa Rose is a young Labrador retriever. Like most labs, Pippa is ... partial tear to the cruciate ligament in her right knee. According to her mom, ... Kinsley reported that it broke her heart to see Pippa lie around in pain because ...
(Date:10/29/2018)... ... 29, 2018 , ... PracticeMatch , a company that provides the nation’s ... career fairs planned for 2019. The company has expanded its offering of virtual (online) ... areas of the country which have the highest concentrations of resident and fellow physicians. ...
(Date:10/24/2018)... ... October 22, 2018 , ... uBiome, the ... of the Data Science Institute, Stephenson Chair of Data Science, and Professor in ... the board of leading scientists and doctors from around the world, Dr. Bourne ...
Breaking Biology Technology: