Navigation Links
Gold nanoparticles could improve antisense cancer drugs

In the fight against cancer, antisense drugs, which prevent genes from producing harmful proteins such as those that cause cancer, have the promise to be more effective than conventional drugs, but the pace of development of these new drugs has been slow.

Using gold nanoparticles combined with DNA, scientists at Northwestern University now have demonstrated a new method for developing antisense drugs that outperform conventional antisense agents. The findings will be published May 19 in the journal Science.

A major challenge has been delivering antisense drugs to cells inside the body while avoiding their break down along the way. The Northwestern team shows that by attaching multiple strands of antisense DNA to the surface of a gold nanoparticle (forming an "antisense nanoparticle") the DNA becomes more stable and can bind to the target messenger RNA (mRNA) more effectively than DNA that is not attached to a nanoparticle surface (as in commercial agents).

When compared to antisense DNA complexed with commercial agents such as Lipofectamine and Cytofectin, the antisense nanoparticles were more effective in gene knockdown (decreasing gene expression and protein production), were less susceptible to degradation resulting in longer lifetimes, exhibited lower toxicity and were more readily absorbed by cells, exhibiting a greater than 99 percent uptake.

"When mutations in the body's genetic material cause too many copies of certain proteins, cancer and other diseases can result," said Chad A. Mirkin, director of Northwestern's Center for Cancer Nanotechnology Excellence, who led the study. "Whereas typical drugs target the proteins, it is possible through gene therapy to target the genetic material itself before it is ever made into copies of harmful proteins. One way to target the genetic material is to block the messenger RNA by using 'antisense DNA,' which prevents the message from ever becoming a protein."

Once inside cells, the DNA-modified nanoparticles act as messenger RNA "sponges" that bind to their targets and prevent them from being converted into proteins.

In their experiments the researchers targeted mRNA sequences that code for enhanced green fluorescent protein (EGFP) expressed in a mouse cell. The antisense sequence of the DNA attached to the nanoparticles was complementary to the mRNA for EGFP expression. When the nanoparticles were introduced to the cells the fluorescence dimmed -- a result of the nanoparticles binding to the mRNA and shutting down the protein's expression, or fluorescence.

"In the future, this exciting new class of antisense material could be used for the treatment of cancer and other diseases that have a genetic basis," said Mirkin, who is George B. Rathmann Professor of Chemistry, professor of medicine and professor of materials science and engineering.

In addition to Mirkin, other authors on the Science paper are Nathaniel L. Rosi (co-first author), David A. Giljohann (co-first author), C. Shad Thaxton, Abigail K. R. Lytton-Jean and Min Su Han, all from Northwestern University.


'"/>

Source:Northwestern University


Related biology news :

1. Probing the promise and perils of nanoparticles
2. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma
3. Using nanoparticles, in vivo gene therapy activates brain stem cells
4. NJIT study shows nanoparticles could damage plant life
5. MIT nanoparticles may help detect, treat tumors
6. Quantum dots reviewed -- Could these nanoparticles hold the cure to cancer?
7. Electric jolt triggers release of biomolecules, nanoparticles
8. U-M researchers use nanoparticles to target brain cancer
9. Gold nanoparticles prove to be hot stuff
10. Widely used iron nanoparticles exhibit toxic effects on neuronal cells
11. Coated nanoparticles solve sticky drug-delivery problem
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/15/2017)... , Aug. 15 2017   ivWatch LLC , a medical ... (IV) therapy, today announced receipt of its ISO 13485 Certification, the ... the International Organization for Standardization (ISO®). ... ivWatch Model 400 Continuous Monitoring device for the early detection ... "This is an important ...
(Date:7/20/2017)... DAL ) customers now can use fingerprints instead of their boarding ... ... biometrics to board aircraft at Reagan Washington National Airport ... Delta,s biometric boarding pass experience that launched in May at the ... to allow eligible Delta SkyMiles Members who are enrolled in CLEAR to ...
(Date:6/30/2017)... 2017 Today, American Trucking Associations announced ... face and eye tracking software, became the newest ... "Artificial intelligence and advanced sensing ... a driver,s attentiveness levels while on the road.  ... detect fatigue and prevent potential accidents, which could ...
Breaking Biology News(10 mins):
(Date:8/16/2017)... and OXFORD, England , Aug. 16, ... consortium for biotech executive search and leadership development, and Virdis ... sectors, have created an exclusive alliance that enables clients to ... "For our clients here in the ... unparalleled access to a diverse population of leadership talent throughout ...
(Date:8/15/2017)... ... 15, 2017 , ... Any expert in stem cell research or stem cell ... than half a century. Despite their essential roles in human health and regenerative ... molecular tags developed for this purpose also tag other, more abundant, non-stem tissue cells ...
(Date:8/14/2017)... (PRWEB) , ... August 14, 2017 , ... ... characterized and performing antibodies. Key researchers in the antibody community have recently come ... and consistency for antibodies in the laboratory. , The team at ...
(Date:8/11/2017)... ... August 11, 2017 , ... Algenist continues to disrupt the skincare ... like never before. , Collagen is the key structural element skin needs to ... Collagen™, which include: , First to market with proprietary ...
Breaking Biology Technology: