Navigation Links
GlycoFi and Dartmouth report full humanization of yeast glycosylation pathway in Science

For the first time, scientists have engineered yeast cells capable of producing a broad repertoire of recombinant therapeutic proteins with fully human sugar structures (glycosylation). These sugar structures ensure a glycoprotein's biological activity and half-life and to date, have necessitated the expression of therapeutic glycoproteins in mammalian hosts. The accomplishment reported today has the potential to eliminate the need for mammalian cell culture, while improving control over glycosylation, and improving performance characteristics of many therapeutic proteins.

Scientists from GlycoFi, Inc., a wholly owned subsidiary of Merck & Co., Inc., and their collaborators at Dartmouth-Hitchcock Medical Center published their report detailing the genetic engineering of the yeast Pichia pastoris to secrete human glycoproteins with fully complex, terminally sialyated N-glycans today in the September 8, 2006 issue of the journal Science. To demonstrate the utility of the engineered yeast strains, recombinant erythropoietin (Epo), a protein that stimulates the production of red blood cells, was expressed, purified and its activity demonstrated in vivo.

"Re-engineering the yeast glycosylation system to fully replicate the full repertoire of human glycosylation reactions has been a challenging six year effort that many doubted could be accomplished," said Tillman Gerngross, Ph.D., chief scientific officer of GlycoFi and professor of Bioengineering at Dartmouth College.

"This achievement required both the elimination of yeast-specific glycosylation reactions and the introduction of 14 heterologous genes, making this one of the most complex cellular engineering endeavors reported to date", said Stephen Hamilton, Ph.D., the lead author of the study and a senior scientist at GlycoFi.

Yeast offers numerous advantages as a recombinant protein expression system when compared to mammalian cell culture. These include the capability
'"/>

Source:JKureczka@comcast.net


Page: 1 2

Related biology news :

1. GlycoFi announces the first production of antibodies with human glycosylation in yeast
2. Dartmouth study finds that arsenic inhibits DNA repair
3. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
4. Surprising findings reported about iron overload
5. Researchers report new pro-inflammatory role for anti-inflammatory enzyme
6. Biochemists report discovery of structure of major piece of telomerase; implications for cancer
7. Leprosy genome tells story of human migrations, French researchers report in Science
8. Novel live reporting system to track cells
9. Biased reporting found in cancer prognostic studies
10. New lead reported in tumor angiogenesis
11. Researchers report breakthrough against world’s deadliest viruses
Post Your Comments:
(Date:6/23/2015)... , June 23, 2015   MedNet ... that supports the entire spectrum of clinical research, ... ™ , the company,s intuitive, flexible ... awarded a Silver 2015 Stevie® Award by the ... Products & Services Website category.  The American Business ...
(Date:6/18/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... commerce market announces that its Wocket® smart wallet will be ... Mark", scheduled to air on WABC Radio (am770) in ... The broadcast air- time for the extensive ... NXT-ID, Inc.,s CEO Gino Pereira will be discussing ...
(Date:6/16/2015)... QUEBEC CITY , June 16, 2015 /CNW ... announce their partnership centralized around the incorporation of ... mobile point-of-care diagnostic laboratory, the Mo-POD™. This unprecedented ... the BIO International Conference at the ... from June 15 th to ...
Breaking Biology News(10 mins):MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 2handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 3
... The largest genome-wide association study ever conducted on ... has identified 16 new genetic associations related to ... 23andMe, the leading personal genetics company, and the ... examined data for more than 53,000 individuals. The ...
... who give birth vaginally are at increased risk of developing ... a study of Chinese women by researchers at Yale School ... online July 1 in the British Journal of Obstetrics ... labor and delivery made the pelvic floor relax and not ...
... that the key to treating neurodegenerative prion diseases such ... in the ribosome, the protein synthesis machinery of the ... Journal of Biological Chemistry . Prion diseases are ... Examples of prion diseases are scrapie in sheep, mad ...
Cached Biology News:23andMe and ALSPAC identify 16 new genetic associations for pollen, dust-mite and cat allergies 223andMe and ALSPAC identify 16 new genetic associations for pollen, dust-mite and cat allergies 3Vaginal delivery ups risk of pelvic organ prolapse 2The ribosome -- a new target for antiprion medicines 2
(Date:7/1/2015)... July 1, 2015  Axovant Sciences Ltd. (NYSE: ... the treatment of dementia, today announced that the ... 22 at 5:45 p.m. EDT following its presentation ... International Conference 2015 (AAIC). The ... , Dr. Lawrence Friedhoff , Chief Development ...
(Date:7/1/2015)... IN (PRWEB) , ... July 01, 2015 , ... Apex ... 2015 BIO International Convention in Philadelphia, PA. , The presentation took place ... and outlined the plans for the development of APX3330 for the treatment of pancreatic ...
(Date:6/30/2015)... ... June 30, 2015 , ... Students ... part in the first half of “Integrative Biotechnology,” an applied biotechnology course team-taught ... summer 2014, this course combines students from both universities and is taught on ...
(Date:6/30/2015)...  Juniper Pharmaceuticals, Inc. (Nasdaq: JNP ), a ... address unmet medical needs in women,s health, today ... present at the Cantor Fitzgerald Inaugural Healthcare Conference ... 4:45 PM EDTLocation: , Le Parker Meridien Hotel, ... www.juniperpharma.com, under  ,Investor, or  click here ...
Breaking Biology Technology:Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Apex Therapeutics Presented Company Overview at This Year’s BIO International Convention in Philadelphia, PA 2Rivier University and Beijing City University Academic and Cultural Exchanges Begin 2Juniper Pharmaceuticals to Present at Cantor Fitzgerald Inaugural Healthcare Conference on July 8, 2015 2
... , , ROCKVILLE, Md. , ... of the PARADIGM trial, a Phase 2 clinical study ... of individuals with suspected central nervous system infection due ... held biotechnology company that develops immunotherapeutics to treat autoimmune ...
... SYDNEY, July 12 /PRNewswire-Asia/ -- Pharmaxis (ASX: ... to voluntarily de-list from the Nasdaq Global,Market ("Nasdaq"). , ... review of the demand from existing and,potential international investors ... on Nasdaq and the volume of Pharmaxis ADS trading,in ...
... ,PROVIDENCE, R.I. [Brown University] Rashid Zia, assistant professor ... winners of the Presidential Early Career Awards for Scientists ... of winners this week. The PECASE award recognizes ... show exceptional potential for leadership at the frontiers of ...
Cached Biology Technology:MacroGenics Announces the Initiation of a Phase 2 Clinical Trial to Evaluate the Safety and Efficacy of Monoclonal Antibody MGAWN1 in Subjects with Suspected Central Nervous System Infection Due to West Nile Virus 2MacroGenics Announces the Initiation of a Phase 2 Clinical Trial to Evaluate the Safety and Efficacy of Monoclonal Antibody MGAWN1 in Subjects with Suspected Central Nervous System Infection Due to West Nile Virus 3MacroGenics Announces the Initiation of a Phase 2 Clinical Trial to Evaluate the Safety and Efficacy of Monoclonal Antibody MGAWN1 in Subjects with Suspected Central Nervous System Infection Due to West Nile Virus 4Pharmaxis to Voluntarily De-List from Nasdaq 2Brown engineering professor wins prestigious White House award 2
... (Human Exonic Evidence Based Oligonucleotide) Genome Set ... ) Genome Set contain a collection of ... constitutively expressed exons, allowing interrogation of thousands ... researchers at Stanford University and UCSF, the ...
odz, odd Oz/ten-m homolog 1 [Source:RefSeq_peptide;Acc:NP_055068] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Rabbit polyclonal to FANCE ( Abpromise for all tested applications). entrezGeneID: 2178 SwissProtID: Q9HB96...
Rabbit polyclonal to NIRF ( Abpromise for all tested applications). Antigen: Synthetic peptide derived from residues 186-235 of human NIRF. Entrez Gene ID: 115426 Swiss Protein ID: Q96PU...
Biology Products: