Navigation Links
'Genetic network' guards against lethal DNA damage

lled synthetic fitness or lethality interactions. Synthetic lethality is a phenomenon in which two mutations that are not individually lethal cause cell death when combined. Specifically, the Hopkins team identified 4,956 interactions among 875 genes involved in DNA repair, DNA replication, the halting of replication and cell cycle progression by "checkpoints" so that damaged DNA can undergo repair, and responses to oxidative stress necessary for reducing the intracellular levels of highly reactive molecules that bind to and damage DNA.

The yeast has about 6,000 genes, of which about 1,000 are essential to survival and 5,000 are not, Boeke said. Specifically, 1,000 of the 5,000 non-essential genes are important enough so that the yeast grows slowly if any one of them is absent. And any of the 4,000 other genes can be deleted from the cell without interfering with the cell's growth.

A major goal of the Hopkins team is to determine which of the non-essential genes interact with each other, said Boeke. All such pair-wise combinations of the 5,000 non-essential genes in the yeast genome would require about 25 million tests, he added. In the current study, 74 genes were tested in pair-wise combination with the 5,000 non-essential genes, a feat approximately equivalent to 370,000 gene-pair tests.

The Hopkins team used a technology known as dSLAM (heterozygote diploid-based synthetic lethality analyzed by microarray) to look at the effects of 5,000 different double mutations on cell fitness in a single experiment. With this technology, only 5,000 tests would be required to map the 25 million pair-wise combinations, greatly speeding the work.

The dSLAM strategy is somewhat like pulling out parts of a radio at random to see what happens, Boeke said.

"With yeast, as with a radio, you might rip out part A or part B and find that the radio still works; but if you pull out both parts and the radio dies you would learn that A and B can comp
'"/>

Source:Johns Hopkins Medical Institutions


Page: 1 2 3

Related biology news :

1. Enzyme, lost in most mammals, is shown to protect against UV-induced skin cancer
2. Bound for destruction: Ubiquitination protects against improper Notch signaling
3. Female sex hormones play a vital role in defense against sexually transmitted diseases
4. In the sea slugs defense against lobsters, confusion is key
5. Antibodies from plants protect against anthrax
6. Molecular models advance the fight against malaria
7. Sickle cell and protection against malaria
8. Live vaccines more effective against horse herpes virus
9. Turning viruses into allies against cancer
10. Friendly bacteria in humans may protect against HIV
11. NSAID drug protects against intestinal tumors in mice, despite poor diet and gene losses
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genetic network guards against lethal DNA damage

(Date:2/5/2015)... Jan. 28, 2015 NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile ... RedChip Global Online CEO Conference on January 29 th ... Gino Pereira , CEO of NXT-ID will present and ... the Wocket smart wallet and its full suite of ...
(Date:2/5/2015)... , Feb. 3, 2015 Despite a ... from industries such as consumer electronics, automotive, storage ... surface mount technology (SMT) screen printers. Innovations in ... control will push the adoption curve up. Meanwhile, ... steady as glue dispensers are indispensable in the ...
(Date:1/22/2015)... TORONTO , Jan. 13, 2015 /PRNewswire/ - Today, FindBiometrics, the ... announced the launch of its new website design. ... in its infancy", says Peter O,Neill , founder and ... ever, the industry needs involvement from the key players on ...
Breaking Biology News(10 mins):CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 3End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2
... F. Laurance, senior staff scientist at the Smithsonian Tropical ... the Institute and Biodiversity Chair at the H. John ... have won the coveted 2008 BBVA Foundation Frontiers of ... 30 Jan. 2009 in Madrid, Spain. , ...
... deposited iron in the Southern Ocean has filled in ... in locking atmospheric carbon dioxide (CO2) in the ocean. ... Raymond Pollard of the National Oceanography Centre, Southampton, and ... Hole Oceanographic Institution (WHOI), found that natural iron fertilization ...
... many studies refuting the claim of a link between vaccines ... issue of Clinical Infectious Diseases and now available ... epidemiological and biological studies refute these claims. "When one ... springs up to take its place," said study author Paul ...
Cached Biology News:Smithsonian scientists receive coveted BBVA Ecology and Conservation Award 2Ocean islands fuel productivity and carbon sequestration through natural iron fertilization 2Ocean islands fuel productivity and carbon sequestration through natural iron fertilization 3Vaccines and autism: Many hypotheses, but no correlation 2
(Date:3/2/2015)... MIAMI (PRWEB) March 02, 2015 ... a newly formed alliance with Shenzhen HANK Bioengineering ... M.D., Ph.D. The alliance will establish a stem ... Guangdong Province, China in September 2015. , Shenzhen ... by Zhang, an award-winning scientist, microbiologist and virologist, ...
(Date:2/27/2015)... Immunovaccine Inc. (“Immunovaccine” or the “Company”) (TSX: ... release disseminated this morning by PR Web, and redistributed ... release states that Immunovaccine has entered into a co-development ... did not originate from Immunovaccine and there is no ... Please note that all official statements by Immunovaccine ...
(Date:2/27/2015)... , Feb. 27, 2015 Research and Markets ... "Global Market Report of Trypsin" report to their offering. ... 9002-07-7) aims at providing comprehensive data on Trypsin globally and ... , North America , Latin ... close attention to Trypsin This report focuses on ...
(Date:2/27/2015)... , Feb. 27, 2015  Pfenex Inc. (NYSE ... development of biosimilar therapeutics, today announced that it will ... Marriott Marquis on March 2 nd at 3pm ... on a panel discussing the current state of the ... Biosimilars . For more information on ...
Breaking Biology Technology:Global Stem Cells Group Announces Alliance with HANK Bioengineering Co., Ltd. in Shenzhen, China 2Global Stem Cells Group Announces Alliance with HANK Bioengineering Co., Ltd. in Shenzhen, China 3Immunovaccine Disclaims Hoax Press Release 2Global Market Report of Trypsin 2014-2018 2
... managed, with the help of an advanced X-ray flash, to ... that has been recognised by the journal Science . ... home to one of the world,s most advanced particle accelerators, ... and study life,s tiniest components: atoms, molecules and proteins. ...
... PLYMOUTH MEETING, Pa. , May 10 /PRNewswire-Asia-FirstCall/ -- ... announced,financial results for the first quarter ended March 31, 2010 . ... ... was a,quarter of successful management transition at Sunstone. We replaced senior,managers including ...
... Fla. , May 10 Dyadic International, Inc. ... today that its President and Chief Executive Officer, Mark ... Renshaw 6th Annual Global Healthcare Conference in London, England ... 11:05 a.m. local time ( 6:05 a.m. EDT ). ...
Cached Biology Technology:BMP Sunstone Reports First Quarter 2010 Financial Results 2BMP Sunstone Reports First Quarter 2010 Financial Results 3BMP Sunstone Reports First Quarter 2010 Financial Results 4BMP Sunstone Reports First Quarter 2010 Financial Results 5BMP Sunstone Reports First Quarter 2010 Financial Results 6BMP Sunstone Reports First Quarter 2010 Financial Results 7BMP Sunstone Reports First Quarter 2010 Financial Results 8BMP Sunstone Reports First Quarter 2010 Financial Results 9BMP Sunstone Reports First Quarter 2010 Financial Results 10BMP Sunstone Reports First Quarter 2010 Financial Results 11BMP Sunstone Reports First Quarter 2010 Financial Results 12Dyadic International to Present at Rodman & Renshaw 6th Annual Global Healthcare Conference 2
... automated microplate strip washer for 96- and 384-well ... strip washing capability, the Columbus combines high flexibility ... , Features ,Key features ... or 16 channels, crosswise aspiration, overflow and bottom ...
... solution for all plate washing needs in both ... ,The PW 384 provides an excellent solution for ... Discovery (Assay development, Screening or Lead Optimization) and ... ,The PW 384 has set a standard for ...
... Protein:Protein Interaction Reagent A powerful new ... gained traction as an in vitro method ... of publications feature the exclusive Pierce ProFound ... a variety of issues relating to protein ...
... compact benchtop and stackable multi-function incubator. ... a large display screen to accurately ... circulation inside to evenly distribute temperature ... easily calibrated from the microprocessor controller. ...
Biology Products: