Navigation Links
Genes involved in biofilms

The biologist Alejandro Toledo Arana has identified two new genes that operate as regulators in the formation process of the biofilm of Staphylococcus aureus, one of the bacteria most frequently involved in infections following medical implants, and has explained the functioning of a structural protein involved in this process. His research was the subject of a PhD thesis recently defended at the Institute of Agribiotechnology, a joint CSIC and Public University of Navarra centre, and is an advance in the race to identify action targets for the development of pharmaceutical drugs to combat these infections.

The PhD, entitled Identification and characterisation of new factors involved in the processes of formation of biofilm from Gram-positive bacteria.

Chronic infections

Biofilms are communities of microorganisms in a matrix that joins them together and to living or inert substrates, points out Alejandro Toledo. Although they are widely found in nature, and in many cases have beneficial effects, their study has been boosted on discovering their relation to chronic infections associated to medical implants such as those tissues involving infections of the middle ear, of the prostate gland, pneumonia in patients with cystic fibrosis, osteomyelitis, etc.

In the interior of the biofilm, bacteria present greater resistance to antibiotics, to the opsonisation by antibodies and to phagocytosis, which explains the chronic character of these infections, states the author of the PhD.

The aim of the PhD was the characterisation of the process of formation of the Staphylococcus aureus biofilm.

Regulating mechanisms unknown to date

The starting point for the research was the Bap protein (Biofilm associated protein). Bap, according to the thesis, presents a structural organisation similar to other surface proteins of unknown function in a number of species of bacteria such as: Esp de Entero coccus faecalis, mus20 of Pseudomonas putida, and sty2875 of Salmonella typhi.

Thus, taking into account the structural homology between Bap and Esp, it was decided to analyse for a possiblerelationship between the presence of the esp gene and the ability to produce biofilm by E. faecalis. The results showed that the presence of the esp gene was involved in the formation processes of these biofilms.

Two new genes

The results obtained to date intuitively suggest the existence of various mechanisms for forming biofilm as a function of the origin of the bacteria strains, as a consequence of which it was decided to widen the scope of the research, using a strain of Staphylococcus aureus from otitis media.

For the identification of the genes involved in the formation of the biofilm of this strain, two different strategies were followed. The first, following the usual methods, involved identifying mutants that had lost the capacity to form biofilm, thus enabling identification of the essential genes and positive regulators of the process. The results showed that a positive regulator known as Pnp (Polynucleotide phosphorylase) exists and which regulates the accumulation of the main exopolysaccharide involved in the formation of the biofilm for this bacteria.

Moreover, the second strategy arose during the development of a medium chemically defined in order to study how environmental signals affect the process of formation of the biofilm. Unexpectedly, most of the clinical strains of Staphylococcus aureus were unable to form biofilm in the synthetic medium, which suggested to us that the process was, perhaps, repressed in this medium.


'"/>

Source:Elhuyar Fundazioa


Related biology news :

1. Genes In The Interferon System Important In Systemic Lupus Erythematosus
2. Protein Packages Found To Activate Genes; May Be What Regulates Development And Disease
3. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
4. Genpathway and Baylor College of Medicine Identify New Genes in Breast Cancer
5. Where Bacteria Get Their Genes
6. Genes linked to treatment resistance in children with leukemia
7. Noise And Delays Explain Why Some Genes Oscillate In Activity
8. Genes involved in cell growth and cell division identified
9. Genes affecting blood pressure change as children become adults
10. Genes discovered that allow gum disease bacterium to invade arteries
11. Genes and genius: Researchers confirm association between gene and intelligence
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... -- Research and Markets has announced the addition of ... their offering. The report forecasts ... grow at a CAGR of 12.28% during the period 2016-2020. ... market analysis with inputs from industry experts. The report covers the ... report also includes a discussion of the key vendors operating in ...
(Date:6/22/2016)... , June 22, 2016  The American College of ... Trade Show Executive Magazine as one of the fastest-growing ... May 25-27 at the Bellagio in Las Vegas ... the highest percentage of growth in each of the following ... exhibiting companies and number of attendees. The 2015 ACMG Annual ...
(Date:6/22/2016)... , June 22, 2016   ... management and verification solutions, has partnered with ... software solutions for Visitor Management, Self-Service Kiosks ... provides products that add functional enhancements to ... provides corporations and venues with an automated ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... , June 27, 2016  Alex,s Lemonade Stand ... that that it will open a state-of-the-art bioinformatics lab, ... cancer research. This announcement comes as Liz Scott ... the National Cancer Moonshot Summit in Washington, ... Biden as a participant and advocate of pediatric cancer ...
(Date:6/27/2016)...  Global demand for enzymes is forecast to ... $7.2 billion.  This market includes enzymes used in ... production, animal feed, and other markets) and specialty ... and beverages will remain the largest market for ... products containing enzymes in developing regions.  These and ...
(Date:6/27/2016)... GUELPH, ON , June 27, 2016 /PRNewswire/ - BIOREM ... it has been advised by its major shareholders, Clean ... LP, United States based venture ... common shares of Biorem (on a fully diluted, as ... for the disposition of their entire equity holdings in ...
(Date:6/27/2016)... June 27, 2016  Sequenom, Inc. (NASDAQ: ... healthier lives through the development of innovative products and ... the United States denied its petition ... claims of Sequenom,s U.S. Patent No. 6,258,540 (",540 Patent") ... established by the Supreme Court,s Mayo Collaborative Services v. ...
Breaking Biology Technology: