Navigation Links
Gene-bender proteins may sway to DNA

Among the many genes packed into each cell of our body, those that get turned on, or expressed, are the ones that make us who we are. Certain proteins do the job of regulating gene expression by clasping onto key spots of DNA -- the nucleic acid that contains the genetic instructions.

How does the protein recognize a particular binding site" Structural changes in both the protein and DNA, sometimes with the DNA within the complex kinked or sharply bent, allow for the specific contacts needed for a tight DNA-protein fit.

Scientists think DNA is largely passive in this genetic tango. But new findings by Anjum Ansari, associate professor of biophysics at the University of Illinois at Chicago, suggest DNA may not be the wallflower that many had assumed.

To follow in real time the structural changes that accompany protein-DNA binding, Ansari and her UIC colleagues used a test protein from bacteria and applied a laser pulse lasting about 10 billionths of a second to heat up and disturb the protein-DNA complex. They watched the dynamics of the bound DNA in response to this perturbation.

Ansari's group was the first to apply the laser temperature-jump technique to study the dynamics of a protein-DNA complex.

The studies were done in collaboration with Donald Crothers, Sterling Professor Emeritus of chemistry at Yale University, who examined the protein-DNA interaction with the more traditional stopped-flow technique.

"While stopped-flow technique can capture dynamics of biomolecules occurring on millisecond time-scales or longer, the goal of this study was to extend the time-resolution down to sub-microseconds. It gave us a new time window on probing protein-DNA interactions," Ansari said.

That broader time window, obtained in combination with the stopped-flow measurements, provided the first direct observation of DNA bending when bound to a DNA-bending protein.

"We found that the time-scales on which D
'"/>

Source:University of Illinois at Chicago


Page: 1 2

Related biology news :

1. New, automated tool successfully classifies and relates proteins in unprecedented way
2. UWs Rosetta software to unlock secrets of many human proteins
3. Global analysis of membrane proteins
4. UNC plant researchers discover proteins interact to form hair-trigger protection against invaders
5. Mad cow proteins successfully detected in blood
6. Researchers develop new method for facile identification of proteins in bacterial cells
7. Hopkins scientists uncover tags that force proteins to cell surface
8. Researchers create functioning artificial proteins using natures rules
9. UCSD discovery may provide novel method to generate medically useful proteins
10. Prostate cancer uses Wnt signaling proteins to promote growth of bone tumors
11. A real time look at interactions between RNA and proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Gene bender proteins may sway DNA

(Date:9/18/2014)... in DNA, tiny strands of nucleic acid that contain ... this genetic data, our DNA is copied into RNA ... perform tasks in our cells. , Several years ... Unlike all other known RNAs, this molecule is circular, ... abundant, little has been known about how they are ...
(Date:9/18/2014)... ALEXANDRIA, Va.Sept. 18, 2014Many Americans across racial and ... the greatest impact on their day-to-day life, more ... memory, hearing and speech (57% of African-Americans, 49% ... of Hispanics). When asked which disease or ailment ... blindness ranked first among African-Americans followed by AIDS/HIV. ...
(Date:9/18/2014)... have devastated algal forests in the eastern Mediterranean Sea ... if their distribution continues to expand as the climate ... international team of researchers led by Dr Adriana Vergs ... Mediterranean Institute for Advanced Studies in Spain, is published ... the team surveyed more than 1000 kilometres of coastline ...
Breaking Biology News(10 mins):Scientists find how mysterious 'circular RNA' is formed, claim muscular dystrophy link 2Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3Tropical fish a threat to Mediterranean Sea ecosystems 2
... of life -- kids get sick and have fevers. ... but in some small children they spark convulsions called ... very upsetting to parents," said Robert L. Macdonald, M.D., ... something of an enigma, he said. The epilepsy research ...
... development of drug-resistant HIV when patients take as few as ... only when patients take most of their pills. These differences ... "fitness" of the drug-resistant HIV, say AIDS researchers in a ... MPH, an AIDS specialist at the University of California, San ...
... Vaccine and Infectious Disease Organization (VIDO) have developed a vaccine ... against a disease for which no vaccines are yet available. ... this vaccination technique may be effective against HCV. The study ... , The team, funded by the Canadian Institutes of Health ...
Cached Biology News:Molecular mechanism may explain how fevers spark seizures 2Molecular mechanism may explain how fevers spark seizures 3Viral 'fitness' explains different resistance patterns to aids drugs 2U of S researchers develop new vaccine candidate against hepatitis C 2
(Date:9/18/2014)... (PRWEB) September 18, 2014 Bipartisan passage ... a bill designed to stimulate development and commercialization of ... being praised by leaders of SPIE, the international ... Revitalizing American Manufacturing and Innovation (RAMI) Act, now moves ... authors have said they are optimistic that it will ...
(Date:9/18/2014)... September 18, 2014 A novel robotic ... MRI scanner is currently being tested as part of ... Hospital in Boston with the aim of determining if ... make prostate cancer biopsies faster, more accurate, less costly, ... also has the potential to deliver prostate cancer therapies ...
(Date:9/18/2014)... On Tuesday, Secretary of Energy Ernest Moniz joined ... Lab Day on the Hill. The event highlighted several ... Durbin and Risch also formally launched the Senate National ... reach of the national labs as leaders in developing ... our nation's most pressing challenges. , "The national labs ...
(Date:9/18/2014)... Research and Markets has announced the addition ... In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to ... The global companion diagnostics market is expected to grow ... Factors such as rising need for personalized medicine in ... pharmaceutical industry, support from regulatory authorities, and emerging technologies ...
Breaking Biology Technology:House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 4Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 5Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 6Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 2Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 3Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 2Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 3
... SAN DIEGO, Oct. 28 Neurocrine Biosciences,(Nasdaq: NBIX ) announces the ... 2008 Financial Results, When: October 28, 2008 @ ... http://www.videonewswire.com/event.asp?id=52783 , How: Live over the Internet ... above., Contact: Claudia ...
... U.S. Phase II Study,of Nitazoxanide in Treatment-Naive Patients ... Oct. 28 Romark Laboratories, a privately,held biopharmaceutical ... at the upcoming 59th Annual Meeting of the ... also known as The,Liver Meeting(R), and the 50th ...
... Ziplex automated gene-expression system at the ... ... Molecular, a pioneer in,the development of cost-effective, easy-to-use gene-expression analysis,systems, ... L. Wittliff, a professor in the Department of,Biochemistry and Molecular ...
Cached Biology Technology:Romark Laboratories Announces Presentation of New Nitazoxanide Data at 59th Annual AASLD Meeting and 50th Anniversary IASL Meeting in San Francisco 2James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 2James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 3
... EVO96R and Freedom EVO384R are fast multi-channel ... in three different sizes, with a multi-channel ... deliver significant time saving, with true parallel ... setup of your assays with the Freedom ...
... are fast multi-channel pipetting platforms consisting of ... with a multi-channel head and a robotic ... with true parallel processing of sample plates ... with the Freedom EVOware program. ...
Prepared by multi-temperature ethanol fractionation with extensive charcoal treatment and dialysis.• Assay: ≥ 95 % (HPCE)• pH ~5.2 (10 mg/mL in 0.15 M NaCl)...
... Linkage: This CLS number is a ... match Cornings product number. If showing ... the old Sigma-Aldrich number (Z71,405-4) or ... Dim: diam. 60 mm volume ...
Biology Products: