Navigation Links
Gene-bender proteins may sway to DNA

Among the many genes packed into each cell of our body, those that get turned on, or expressed, are the ones that make us who we are. Certain proteins do the job of regulating gene expression by clasping onto key spots of DNA -- the nucleic acid that contains the genetic instructions.

How does the protein recognize a particular binding site" Structural changes in both the protein and DNA, sometimes with the DNA within the complex kinked or sharply bent, allow for the specific contacts needed for a tight DNA-protein fit.

Scientists think DNA is largely passive in this genetic tango. But new findings by Anjum Ansari, associate professor of biophysics at the University of Illinois at Chicago, suggest DNA may not be the wallflower that many had assumed.

To follow in real time the structural changes that accompany protein-DNA binding, Ansari and her UIC colleagues used a test protein from bacteria and applied a laser pulse lasting about 10 billionths of a second to heat up and disturb the protein-DNA complex. They watched the dynamics of the bound DNA in response to this perturbation.

Ansari's group was the first to apply the laser temperature-jump technique to study the dynamics of a protein-DNA complex.

The studies were done in collaboration with Donald Crothers, Sterling Professor Emeritus of chemistry at Yale University, who examined the protein-DNA interaction with the more traditional stopped-flow technique.

"While stopped-flow technique can capture dynamics of biomolecules occurring on millisecond time-scales or longer, the goal of this study was to extend the time-resolution down to sub-microseconds. It gave us a new time window on probing protein-DNA interactions," Ansari said.

That broader time window, obtained in combination with the stopped-flow measurements, provided the first direct observation of DNA bending when bound to a DNA-bending protein.

"We found that the time-scales on which D
'"/>

Source:University of Illinois at Chicago


Page: 1 2

Related biology news :

1. New, automated tool successfully classifies and relates proteins in unprecedented way
2. UWs Rosetta software to unlock secrets of many human proteins
3. Global analysis of membrane proteins
4. UNC plant researchers discover proteins interact to form hair-trigger protection against invaders
5. Mad cow proteins successfully detected in blood
6. Researchers develop new method for facile identification of proteins in bacterial cells
7. Hopkins scientists uncover tags that force proteins to cell surface
8. Researchers create functioning artificial proteins using natures rules
9. UCSD discovery may provide novel method to generate medically useful proteins
10. Prostate cancer uses Wnt signaling proteins to promote growth of bone tumors
11. A real time look at interactions between RNA and proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Gene bender proteins may sway DNA

(Date:3/23/2015)... SoundView Technology Group issues a new research update ... Wocket smart wallet. SoundView was one of the selected user groups ... the Wocket in multiple scenarios and outlets. ... retailers, making both debit and credit card payments.  ... the company meets their plans in 2015, it would push ...
(Date:3/23/2015)... , Mar. 23, 2015 NXT-ID, Inc. (NASDAQ: ... focused on the growing mobile commerce market, announces its biometric ... advertising campaign on CNBC television starting March 30 th ... commence airing in New York markets. ... said: "We are excited about our new ad campaign following ...
(Date:3/20/2015)... , March 20, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... is one of the products featured in 2015 "I ... at 11:00 PM EST on the DIY Network.   ... Las Vegas , site of the 2015 ...
Breaking Biology News(10 mins):Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 2NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 3
... An experimental drug that blocks two points of ... of ovarian cancer cells and significantly increases survival in ... Jonsson Comprehensive Cancer Center has found. The drug, ... that have become resistant to the conventional treatment with ...
... use of stored blood during transfusions may cause adverse effects ... funded a number of research projects to examine the safety ... blood on respiratory gases. These papers discussing potential adverse effects ... transfusion are now available online in TRANSFUSION , a ...
... a novel approach that works around the gene defect in Wiskott-Aldrich ... signaling pathway to significantly improve immune function in a 13-year-old boy ... provides a proof-of-principle that immunotherapy, which harnesses elements of the body,s ... deadly disorder. ...
Cached Biology News:Experimental drug inhibits cell signaling pathway and slows ovarian cancer growth 2Safety of stored blood among chief concerns for transfusion medicine community 2Novel therapy improves immune function in teen with rare disease 2
(Date:3/26/2015)... (PRWEB) March 26, 2015 PRC Clinical, ... announced today several management and executive appointments effective immediately. ... Financial Officer (CFO) and member of the Board of ... Business Operations, and Sue Dowden will assume the position ... and current Chief Operations Officer (COO), has been appointed ...
(Date:3/26/2015)... Corporation (NASDAQ: NEOG ) announced today that its ... which ended Feb. 28, increased 13% to $7,454,000, when ... in the current quarter were $0.20, compared to $0.18 ... over prior year to $24,142,000, or $0.65 per share, ... same period a year ago. Revenues for ...
(Date:3/26/2015)... NJ (PRWEB) March 26, 2015 WriteResult, ... partnered with researchers from Yale University’s School of Public ... by study participants during a 4 month-long project. The ... of providing low income families with free access to ... habits and diet-related health concerns like high blood pressure, ...
(Date:3/25/2015)... London, UK (PRWEB) March 25, 2015 ... pervasive uptake of smartphones have fuelled social media ... population being currently engaged in social networking. Insurance ... exhaustively to communicate with clients and promote a ... the globe over are increasingly exploiting social networking ...
Breaking Biology Technology:PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8Yale and New Haven Farms Improving Patient Health with WriteResult's myPROpad™ 2Yale and New Haven Farms Improving Patient Health with WriteResult's myPROpad™ 3Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 2Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 3
... Fla., April 14 Phlo Corporation,(Pink Sheets: PHCP) ... stock certificates are negotiable in connection with,market transactions ... current,position in Phlo common stock. This clarification was ... number of brokers and clearing firms,will not negotiate ...
... in the second quarter of 2008 ... ... Ardea,Biosciences, Inc. (Nasdaq: RDEA ) today announced that preclinical data ... compounds demonstrating potent,activity against the human-immunodeficiency virus (HIV), including NNRTI-,resistant strains, with ...
... basis of blood-based ColonSentry(TM) test -, TORONTO, ... company focused on developing blood-based biomarker tests for ... today reported,positive performance results from a large validation ... of a patient,s current risk for,colorectal cancer. This ...
Cached Biology Technology:Phlo Clarifies That Pre-Reverse Split Certificates Are Negotiable in Market Transactions 2Ardea Presents Preclinical Data on Novel Next Generation Non-Nucleoside Reverse Transcriptase Inhibitors Demonstrating Potent Anti-HIV Activity Against Resistant Viruses 2Ardea Presents Preclinical Data on Novel Next Generation Non-Nucleoside Reverse Transcriptase Inhibitors Demonstrating Potent Anti-HIV Activity Against Resistant Viruses 3GeneNews reports positive results from validation study of colorectal cancer biomarkers in late-breaking abstract at AACR 2GeneNews reports positive results from validation study of colorectal cancer biomarkers in late-breaking abstract at AACR 3
Monkey IL-2 ELISPOT Kit with white/opague microtiter plates...
Tachykinin (H-2)...
Human GATA-2 Affinity Purified Polyclonal Ab...
... microPET 120 utilizies CTI Molecular Imaging's proprietary ... same imaging volume as the R4. ... sensitivity of any commerically avaiable PET scanner. ... spatial resolution of any scinitallor based PETsystems. ...
Biology Products: