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Freeze! Scientists film proteins at work by freezing them at different states

eeze the protein in three different states while the reaction took place. In order to make sure that they “trapped?the right intermediate states, the researchers used the technique of Raman spectroscopy. This technique provided them with strong evidence that the states were the appropriate ones by showing them the chemical bonds in each stage of the reaction. Once they had identified the right states, they studied the sample with synchrotron x-rays. “We expect this new methodology to be of use for many researchers in the field? Bourgeois explains.

Filming certain proteins whilst reactions occur has been possible at the ESRF for some years. However, experiments until today were restricted to proteins that get excited by light and are in very resistant crystals.
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Source:European Synchrotron Radiation Facility


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