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Firefly protein lets researchers monitor molecule linked to cancer

e-response curve, which helps establish how to best use a drug," Piwnica-Worms says. "Establishing that normally takes 6 months and 300 mice. With our monitoring technique, Shimon did it in a 5-day period using 30 mice. That's going to lead to tremendous cost savings."

Because the luciferase-based monitoring system allows monitoring in live animals, Gross could perform multiple tests on the same mouse over time. He was also able to monitor the mice for individual variances that could inappropriately bias the results.

Gross S, Piwnica-Worms D. Real-time imaging of ligand-induced IKK activation in intact cells and in living mice. Nature Methods, August 2005. Funding from the National Institutes of Health.


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Source:Washington University School of Medicine


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