Navigation Links
Firefly protein lets researchers monitor molecule linked to cancer

eviously developed techniques that use luciferase to monitor protein-protein interactions. Researchers can employ an instrument known as an in-vivo bioluminescence camera to take real-time measurements of light from luciferase in cell cultures and in cells within live animals.

To use the firefly protein to monitor IKK, Gross altered cell lines to genetically fuse the luciferase protein to IKB (IKappaB), the protein that comes immediately after IKK in the NF-KappaB pathway. When the pathway is enabled, IKK triggers reactions that lead to the degradation of IKB. In cells with genetically altered IKB, the attached luciferase is broken down too, meaning scientists can detect increased IKK activity via decreased light from the cells.

"This is like doing in-vivo pharmacodynamics and pharmacokinetics," says Piwnica-Worms in reference to the sciences that study the effects, distribution and dissipation of drugs. "Traditionally the only ways we could do those kinds of studies were either to test for levels of the drug in the blood or to label the drug with a radioactive tracer.

"In the case of NF-KappaB, there were also methods that monitored IKK activity via changes in the levels of gene activation at the end of the pathway," he notes. "But those took hours to days to deliver results, and our approach works continuously and in real time." In their study, Gross and Piwnica-Worms tested the technique in live mice by transplanting genetically altered tumor cells and by using a technique that inserted the fused IKB/luciferase protein into liver cells only. They are currently working to develop a line of mice with the IKB/luciferase fusion built into its genetic code.

In addition, they showed that the system is not only helpful for learning if a drug is having the desired effect, it can also be used to fine-tune drug dosage for maximum benefit.

"One of the reviewers of our paper suggested that we should use the system to produce a full dos
'"/>

Source:Washington University School of Medicine


Page: 1 2 3

Related biology news :

1. New, automated tool successfully classifies and relates proteins in unprecedented way
2. New binding target for oncogenic viral protein
3. Controversial drug shown to act on brain protein to cut alcohol use
4. Timing is everything: First step in protein building revealed
5. UWs Rosetta software to unlock secrets of many human proteins
6. Researchers find how protein allows insects to detect and respond to pheromones
7. Signaling protein builds bigger, better bones in mice
8. Ancient olfaction protein is shared by many bugs, offering new pest control target
9. Automatic extraction of gene/protein biological functions from biomedical text
10. Discovery of key proteins shape could lead to improved bacterial pneumonia vaccine
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
(Date:4/23/2014)... Huntington,s disease, most in their mid-thirties and forties, ... behavioral decline within two decades. "Mutant" protein clusters, ... disease, have been the primary focus of therapies ... new research from Prof. Gerardo Lederkremer and Dr. ... Cell Research and Immunology, in collaboration with Prof. ...
(Date:4/23/2014)... evidence that the source of a unique rhythmic sound, ... the "bio-duck," is the Antarctic minke whale ( Balaenoptera ... in the 1960s who thought it sounded like a ... locations in the Southern Ocean, but its source has ... an international team of researchers deployed acoustic tags on ...
(Date:4/23/2014)... The shale gas boom has transformed the energy ... locations, it could cause conflict among the energy ... resources, say researchers. They add that degraded water ... adequate safeguards. The feature article appears in the ... , Meagan S. Mauter and colleagues point out ...
Breaking Biology News(10 mins):On the defensive 2On the defensive 3Scientists identify source of mysterious sound in the Southern Ocean 2Scientists identify source of mysterious sound in the Southern Ocean 3How to avoid water wars between 'fracking' industry and residents 2
... University of Pittsburgh,s Department of Biological Sciences to encourage ... been rewarded with two prestigious grants totaling $2 million ... as the renewed appointment of department chair and Eberly ... Professors nationwide and the only one in Pennsylvania. ...
... in plots to blow up the London subway, among other ... planned to pack some of his bombs with toxic gas. ... his accomplices before they could carry out their attacks. ... failed, at some point someone might succeed. The right ...
... from a shrub called the burning bush, known for ... in biofuels and low-calorie food oils, according to Michigan ... applied to seeds of the species Euonymus alatus -- ... the gene responsible for its manufacture of a novel, ...
Cached Biology News:Pitt's education of aspiring undergrad, high school biologists rewarded with $2 million 2'Scrubbing' chemical-contaminated buildings clean with lasers 2'Scrubbing' chemical-contaminated buildings clean with lasers 3Research promises healthier vegetable oil -- and tractor fuel to harvest it 2
(Date:1/15/2014)... More than 5 million Americans are currently ... seniors will die with Alzheimer’s or another dementia, according ... shocked many Americans into looking for ways to improve ... age-related cognitive disorders. Jonathan Weisman, president of Biohack Pure, ...
(Date:1/15/2014)... Freeslate, Inc ., the leading ... Lupin Limited, one of India’s top five pharmaceutical companies, ... System for high throughput solid form screening. ... a wide range of quality, affordable generic and branded ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company ... under simulated embryonic conditions, today announced that they have ... Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent ... is an amendment to the existing license between Histogen ...
(Date:1/14/2014)... SAN DIEGO and BETHESDA, Md. ... they are joining together with two institutes from the ... more reliable tools for bringing safer, more effective treatments ... with the National Center for Advancing Translational Sciences (NCATS) ...
Breaking Biology Technology:Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3
... firm , Brookfield, Wis. Norlight ... the customer base and business assets of the Michigan-based ... to give Norlights product range, including hosted IP telephony ... Rapids and Lansing regions. , Criticare purchasing orders for ...
... CIO of American Family When Byrne Chapman started his ... he imagined. Yet the business needs at American Family Insurance ... has started handling terabytes of storm data from the national ... paths and match the predictions to the locations of their ...
... gadget guys and girls this is your time. The shops ... season. But when it comes to the value of these devices ... them. I guess I fall into the latter category. , ... (although when I read the Unabombers manifesto several years ago a ...
Cached Biology Technology:American Family CIO takes entrepreneurial approach to innovation 2American Family CIO takes entrepreneurial approach to innovation 3American Family CIO takes entrepreneurial approach to innovation 4More electronic gadgets, more miscommunication 2More electronic gadgets, more miscommunication 3
Request Info...
Purified Mouse anti-Hu LAT (pY226) Storage Temperature: Refrigerate(2 to 8C)...
Molecular Biology grade. Ideally suited for DNA and RNA separation and recovery. High gel strength. Gelling temp. 36C. Melting temp. 88C. EEO: 0.05-0.13 Resolution 0.2-40 kb; Rnase/Dnase: none detec...
...
Biology Products: