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Fantastic Voyage: A new nanoscale view of the biological world

, RNA, sugar and fatty acids at a subcellular level in tissue sections or cells. "Using MIMS, we can image and quantify the fate of these molecules when they go into cells, where they go, and how quickly they are replaced," says Lechene.

The method does not need staining or use of radioactive labelling. Instead, it is possible to use stable isotopes to track molecules. For example, researchers could track stem cells by labelling DNA with 15N. "These stable isotopes do not alter the DNA and are not toxic to people; with MIMS and stable isotope labelling we could track these cells, where they are and how they have changed several years later," says Lechene.

"The most significant feature of this technique is that it opens up a whole new world of imaging; we haven't yet imagined all that we can do with it," says Peter Gillespie from the Oregon Health and Science University in Portland, USA in an accompanying news article, also published today in Journal of Biology.
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Source:BioMed Central


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