Navigation Links
Embryonic stem cells do better on bumpy nanoscale mattress

Nothing in the cellular world is flat. Even the flattest of basement membranes has topography; bumps, if you like, beneath the cellular mattress.

Unlike the princess kept awake by the pea, human embryonic stem (HES) cells do better when cultured on a substrate deliberately printed with nanoscale grooves and ridges, according to researchers from the University of Wisconsin–Madison.

The researchers used soft lithography to stamp polyurethane substrates with a nano- micron scale topography; a rugged cellular landscape ranging from a few billionths to a few millionths of a meter in altitude.

The HES cells in culture seemed to appreciate the bumps. A line of HES cells grown for five days on the artificial ridges and grooves kept their “stemness,?their self-renewing phenotype, far better than HES cells plated on standard flat culture surfaces, according to Daniel McFarlin, K.J. Finn, and Chris Murphy of the University of Wisconsin’s School of Veterinary Medicine, who teamed with P.F. Nealey of the University’s Department of Chemical Engineering.

Unlike stem cells derived from adult tissues, which have a limited number of cell doublings, embryonic stem cells cultured under the right conditions have the potential to divide indefinitely, without losing their pluripotent properties. But until now, HES cell cultures had a tendency to spontaneously differentiate, that is, to veer off without warning into a developmental pathway. HES cell cultures have to be closely watched to remove any of these spontaneously differentiated colonies.

Researchers have looked at surface chemistry, growth factors, and mechanical forces as factors in runaway stem cell differentiation, but topography is a new dimension for HES, say Murphy and McFarlin, and a highly promising one.

This is the first demonstration that the physical topography, using controlled feature dimensions, of cell culture surfaces influences HES ce ll differentiation and self-renewal, according to the researchers. For HES cells to realize their potential in clinical medicine, they would have to be cultured in great quantities and with great fidelity to their pluripotent phenotype. Fine-tuning their nano-micro topography could boost the efficiency of HES cell propagation.


'"/>

Source:American Society for Cell Biology


Related biology news :

1. Transplantation Of Monkey Embryonic Stem Cells Reverses Parkinson Disease In Primates
2. UCSD Discovery Shows How Embryonic Stem Cells Perform Quality Control Inspections
3. Embryonic Stem Cells Treated With Growth Factor Reverse Hemophilia In Mice: UNC Researchers
4. Embryonic stem cells accrue genetic changes
5. Embryonic selection of sex avoids conceiving blind children
6. Embryonic patterning makes the feathers fly
7. Wisconsin scientists grow critical nerve cells
8. Spleen may be source of versatile stem cells
9. Researchers discover way to make cells in the eye sensitive to light
10. Priming embryonic stem cells to fulfill their promise
11. Lack of enzyme turns fat cells into fat burners
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/12/2016)... DALLAS , May 12, 2016 ... has just published the overview results from the Q1 ... of the recent wave was consumers, receptivity to a ... wearables data with a health insurance company. ... choose to share," says Michael LaColla , CEO ...
(Date:4/28/2016)... India , April 28, 2016 ... Infosys (NYSE: INFY ), and Samsung SDS, a ... that will provide end customers with a more secure, ... services.      (Logo: http://photos.prnewswire.com/prnh/20130122/589162 ) , ... services, but it also plays a fundamental part in enabling ...
(Date:4/26/2016)... DUBLIN , April 27, 2016 ... of the  "Global Multi-modal Biometrics Market 2016-2020"  report ... ) , The analysts forecast ... a CAGR of 15.49% during the period 2016-2020.  ... a number of sectors such as the healthcare, ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... (PRWEB) , ... June 27, 2016 , ... Parallel ... clinical trials, announced today the Clinical Reach Virtual Patient Encounter CONSULT module ... circle with the physician and clinical trial team. , Using the CONSULT module, patients ...
(Date:6/27/2016)... BOSTON , June 27, 2016   Ginkgo ... biology to industrial engineering, was today awarded as ... a selection of the world,s most innovative companies. ... at scale for the real world in the ... organism engineers work directly with customers including Fortune ...
(Date:6/24/2016)... ... ... Researchers at the Universita Politecnica delle Marche in Ancona combed medical journal ... Their findings are the subject of a new article on the Surviving Mesothelioma website. ... the blood, lung fluid or tissue of mesothelioma patients that can help point doctors ...
(Date:6/23/2016)... June 23, 2016 /PRNewswire/ - FACIT has announced ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" ... commercialization of a portfolio of first-in-class WDR5 inhibitors ... such as WDR5 represent an exciting class of ... precision medicine for cancer patients. Substantial advances have ...
Breaking Biology Technology: