Navigation Links
Embryonic stem cells accrue genetic changes

technology development by the National Institutes of Health, support that enabled development of the technological infrastructure necessary for large-scale comparative research, particularly the Human Genome Project, says study co-author Mahendra Rao, M.B.B.S., Ph.D., of the Laboratory of Neurosciences at the National Institute on Aging.

The scientists used so-called GeneChip microarrays, or oligonucleotide arrays, to determine whether there were genetic differences between the early and the late batch of each of the stem cell lines, including whether any genes were present in extra copies. Depending on the gene affected, extra copies could lead to accelerated cell growth, increased cell death, or no measurable effect at all.

In addition to probing changes in the nuclear and mitochondrial DNA sequences and copy numbers, the researchers examined whether the cells' genetic material had shifts in marks that sit on genes and are passed from cell to cell during cell division. These so-called epigenetic marks -- in this case methyl groups on a gene region known as the promoter -- help control whether a gene is used by a cell to make proteins. The researchers determined the methylation status of 14 genes in each of the batches of stem cells; three of the genes did show different methylation patterns in late batches compared to early batches.

The scientists' analysis revealed that five of the nine cell lines had extra or fewer copies of at least one section of their genetic material in the late batch compared to the same cell line's early batch. Two of the nine lines had changes in their mitochondrial DNA over time, and all nine stem cell lines exhibited some shift in methylation of at least one of three genes. One of these genes, called RASSF1A, is also methylated in many cancers, but what effect the methylation has on the stem cells is unknown.

The team is already planning to conduct similar analyses of the remaining NIH-approved c
'"/>

Source:Johns Hopkins Medical Institutions


Page: 1 2 3 4

Related biology news :

1. Transplantation Of Monkey Embryonic Stem Cells Reverses Parkinson Disease In Primates
2. UCSD Discovery Shows How Embryonic Stem Cells Perform Quality Control Inspections
3. Embryonic Stem Cells Treated With Growth Factor Reverse Hemophilia In Mice: UNC Researchers
4. Embryonic selection of sex avoids conceiving blind children
5. Embryonic stem cells do better on bumpy nanoscale mattress
6. Embryonic patterning makes the feathers fly
7. Wisconsin scientists grow critical nerve cells
8. Spleen may be source of versatile stem cells
9. Researchers discover way to make cells in the eye sensitive to light
10. Priming embryonic stem cells to fulfill their promise
11. Lack of enzyme turns fat cells into fat burners
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Embryonic stem cells accrue genetic changes

(Date:9/23/2014)... gotten a lot greener thanks to a novel technique ... uses solar energy to accelerate tailings pond reclamation efforts ... a light source to treat oil sands process affected ... El-Din and James Bolton have found that using the ... just as efficiently but at a much lower cost. ...
(Date:9/23/2014)... will award funds to support the next phase of ... ways of predicting drug safety and effectiveness. Researchers will ... tissue chips and combine them into an integrated system ... body. Led by the National Center for Advancing Translational ... $17 million in 2014 with additional support over the ...
(Date:9/23/2014)... Eureka Therapeutics, Inc. announced today that the Company has ... new funding will be used to expand the Company,s ... clinical development. New investor Yuan Capital led the round, ... including Acorn Campus Ventures, Suma Venture and Harbinger Venture ... and development of fully-human antibodies engineered to mimic the ...
Breaking Biology News(10 mins):Solar energy-driven process could revolutionize oil sands tailings reclamation 2NIH funds next phase of Tissue Chip for Drug Screening program 2NIH funds next phase of Tissue Chip for Drug Screening program 3NIH funds next phase of Tissue Chip for Drug Screening program 4
... data wirelessly for more than a year in early stage ... Institutes of Health. In addition to allowing for more natural ... represents a potential major step toward cord-free control of advanced ... is in the April 2013 issue of the Journal ...
... the University of Massachusetts Amherst led by microbiologist Derek ... sulfurreducens conducts electricity very effectively along metallic-like "microbial ... of such a novel biological electron transfer mechanism, but ... behavior represents a paradigm shift. It goes against all ...
... of adoption has taxed the minds of evolutionary scientists since ... According to Richard Dawkins,s description, adoption is "a double ... to increase, your own reproductive success, but you improve someone ... take care of young that are not related to them? ...
Cached Biology News:Wireless, implanted sensor broadens range of brain research 2Wireless, implanted sensor broadens range of brain research 3UMass Amherst researchers reveal mechanism of novel biological electron transfer 2UMass Amherst researchers reveal mechanism of novel biological electron transfer 3Risk management in fish: How cichlids prevent their young from being eaten 2
(Date:9/23/2014)... 2014 News Facts , ... devoted to genome-based research for human health and disease, ... taking a bite out of oral cancer with its world-class ... India with the help of state-of-the-art solution ... (DDN). , To fuel its cutting-edge cancer research, the ...
(Date:9/23/2014)... September 23, 2014 This is ... Global and China Isopropanol (IPA) industry. The report ... definitions, applications and industry chain structure. Global market ... with a focus on history, developments, trends and ... the international and Chinese situation is also offered. ...
(Date:9/23/2014)... Sept. 23, 2014   GenoSpace , a precision ... of tools to enable the broad use of genomic ... it has been awarded Phase I of a Fast ... National Institutes of Health (NIH) to enable enhanced data ... - http://photos.prnewswire.com/prnh/20140922/147649 During the ...
(Date:9/23/2014)... Texas Fertility Center (TFC) announces the opening of ... Central Texas footprint that includes Round Rock, Central Austin, ... the region’s most established practice will provide full service ... Kyle and San Marcos communities. , “We want ... individuals and couples living in and around South Austin,” ...
Breaking Biology Technology:National Institute of Biomedical Genomics (NIBMG) Deploys DataDirect Networks Storage to Achieve Significant Performance Improvement in World-Class Genomics Research and Collaboration for Oral Cancer 2National Institute of Biomedical Genomics (NIBMG) Deploys DataDirect Networks Storage to Achieve Significant Performance Improvement in World-Class Genomics Research and Collaboration for Oral Cancer 3National Institute of Biomedical Genomics (NIBMG) Deploys DataDirect Networks Storage to Achieve Significant Performance Improvement in World-Class Genomics Research and Collaboration for Oral Cancer 4National Institute of Biomedical Genomics (NIBMG) Deploys DataDirect Networks Storage to Achieve Significant Performance Improvement in World-Class Genomics Research and Collaboration for Oral Cancer 5(IPA) Isopropanol Industry International & China Development Trend Analysis Now Available at DeepResearchReports.com 2(IPA) Isopropanol Industry International & China Development Trend Analysis Now Available at DeepResearchReports.com 3GenoSpace Awarded Fast Track SBIR Grant to Enable Data Access and Cohort Construction for Biorepositories 2Opening Doors to a Family: Texas Fertility Center Announces Newest Fertility Clinic in South Austin 2Opening Doors to a Family: Texas Fertility Center Announces Newest Fertility Clinic in South Austin 3
... 6, 2012 Arteriocyte, a leading biotechnology company ... announced today approval from the Food and Drug ... trial using its Magellan MAR01™ technology in the ... Exemption (IDE 15013) allows Arteriocyte and its clinical ...
... clusters of pancreatic beta-cells that live much longer and secrete ... valuable new tools for studying pancreatic diseases such as diabetes ... in an article in Tissue Engineering, Part C, Methods ... . The article is available free online at the ...
... dose of carbon nanotubes more than doubles the growth ... production of everything from lifesaving medications to sweeteners to ... the first to show that carbon nanotubes boost plant ... ACS Nano . Mariya V. Khodakovskaya and colleagues ...
Cached Biology Technology:Arteriocyte Receives FDA Approval to Initiate Compartment Syndrome Trial for Amputation Prevention Focused on Wounded Warriors 2Arteriocyte Receives FDA Approval to Initiate Compartment Syndrome Trial for Amputation Prevention Focused on Wounded Warriors 3
Small bottle for hybridization 150 x 35mm...
... Forma stacking shakers have the ... speed stacked 3-high, and the ... sensitive "soft shake" applications. ... and systems, our stackers are ...
CQCS kit for first KR 4i , (first unit)....
Multidrop Micro high speed 1 l bulk reagent dispenser rapidly, precisely and accurately dispenses microvolumes of 1-50 l into 384- and 96-well microplates....
Biology Products: